RECEIVE 25% OFF PUBLISHED ANTIBODIES WITH CODE "PUB25"! Offer valid for US customers and available internationally through distributors.

Contact us at 1.800.501.7654 or info@biossusa.com

MenuSearch

Products



×

Collection Menu

Catalog #1: bsm-2271M-PE

Applications: IF

Reactivity: Virus, Others

Conjugate: PE

Catalog #1: bsm-2271M-PerCP

Applications: IF

Reactivity: Virus, Others

Conjugate: PerCP

Catalog #1: bsm-2271M-PerCP-Cy5.5

Applications: IF

Reactivity: Virus, Others

Conjugate: PerCP-Cy5.5

Catalog #1: bsm-2271M-RBITC

Applications: IF

Reactivity: Virus, Others

Conjugate: RBITC

Catalog #1: bsm-51227M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Whole cell extracts (40 \u03bcg) from HeLa cells transfected with HDAC2 siRNA (lane 2) and from an untransfected control (lane 1) were analysed by Western blot using HDAC2 (7G5) Monoclonal Antibody (bsm-53026M) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right (expected size: 55 kDa); the marker (in kDa) is shown on the left.
HeLa cells were stained with HDAC2 (7G5) Monoclonal Antibody (bsm-53026M) and with DAPI. Cells were fixed with 4% formaldehyde for 10\u2019 and blocked with PBS\/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labelled with the HDAC2 antibody (left) diluted 1:500 in blocking solution followed by an anti-mouse antibody conjugated to Alexa594. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.
ChIP assays were performed using HeLa cells, HDAC2 (7G5) Monoclonal Antibody (bsm-53026M) and optimized PCR primer sets for qPCR. ChIP was performed using sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2 and 5 \u03bcg per ChIP experiment was analysed. IgG (2 \u03bcg\/IP) was used as negative IP control. QPCR was performed with primers for the ACTB, EIF4A2 and LMO4 genes, used as positive controls, and for the Sat2 satellite repeat, used as negative control. Figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).
ChIP was performed on sheared chromatin from 4 million HeLa cells using 1 \u03bcg of HDAC2 (7G5) Monoclonal Antibody (Cat. No. C15200201) as described above. The IP\u2019d DNA was subsequently analyzed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer\u2019s instructions. The 51 bp tags were aligned to the mouse genome using the BWA algorithm. Figure shows the enrichment along the complete sequence and a 2 Mb region of human chromosome 3 (fig 2A and B), and in a two genomic regions surrounding the LMO4 positive control gene and the MAGEC1 gene (figure 2C and D).

Catalog #1: bsm-53026M

Applications: WB, ELISA, IF(ICC), dot-blot, ChIP-seq

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-51026R

Applications: WB, IF

Reactivity: Human

Conjugate: Unconjugated

The Hela (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.).Primary Antibody (green):Mouse Anti-HDAC3 antibody (bsm-60265M,1:100); Secondary Antibody (white blue): Goat anti-Mouse IgG-FITC (bs-60296G-FITC): 1 \u03bcg\/test. Blank control (black): PBS. Acquisition of 20,000 events was performed.
4% Paraformaldehyde-fixed Hela (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (HDAC3) monoclonal Antibody, unconjugated (bsm-60625M) 1:100, 90 min at 37\u00b0C; followed by conjugated Goat Anti-Mouse IgG antibody (green, bs-60296G-FITC) at 37\u00b0C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.

Catalog #1: bsm-60265m-100ul

Applications: FCM, IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-60742m-100ul

Applications: WB, FCM

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-60261M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded (human uterus); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (HE4 ) Monoclonal Antibody, Unconjugated (bsm-43151M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human breast); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (HE4 ) Monoclonal Antibody, Unconjugated (bsm-43151M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human Ovarian Tumor); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (HE4 ) Monoclonal Antibody, Unconjugated (bsm-43151M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (HE4 ) Monoclonal Antibody, Unconjugated (bsm-43151M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.

Catalog #1: bsm-43151m-100ul

Applications: IHC-P, IHC-F, IF(IHC-P)

Reactivity: Human, Rat

Conjugate: Unconjugated

Catalog #1: bsm-43151m-bf350-100ul

Applications: IF(IHC-P)

Reactivity: Human, Rat

Conjugate: AbBy Fluor-350

Catalog #1: bsm-43151m-bf405-100ul

Applications: IF(IHC-P)

Reactivity: Human, Rat

Conjugate: AbBy Fluor-405

Catalog #1: bsm-43151m-bf488-100ul

Applications: IF(IHC-P)

Reactivity: Human, Rat

Conjugate: AbBy Fluor-488

Catalog #1: bsm-43151m-bf555-100ul

Applications: IF(IHC-P)

Reactivity: Human, Rat

Conjugate: AbBy Fluor-555

Catalog #1: bsm-43151m-bf594-100ul

Applications: IF(IHC-P)

Reactivity: Human, Rat

Conjugate: AbBy Fluor-594

Catalog #1: bsm-43151m-bf647-100ul

Applications: IF(IHC-P)

Reactivity: Human, Rat

Conjugate: AbBy Fluor-647

Catalog #1: bsm-43151m-bf680-100ul

Applications: IF(IHC-P)

Reactivity: Human, Rat

Conjugate: AbBy Fluor-680

Catalog #1: bsm-43151m-bf750-100ul

Applications: IF(IHC-P)

Reactivity: Human, Rat

Conjugate: AbBy Fluor-750

Catalog #1: bsm-43151m-apc-100ul

Applications: IF(IHC-P)

Reactivity: Human, Rat

Conjugate: APC

Catalog #1: bsm-43151m-apc-Cy5.5-100ul

Applications: IF(IHC-P)

Reactivity: Human, Rat

Conjugate: APC-Cy5.5

Catalog #1: bsm-43151m-apc-cy7-100ul

Applications: IF(IHC-P)

Reactivity: Human, Rat

Conjugate: APC-Cy7

Catalog #1: bsm-43151m-biotin-100ul

Applications: IHC-P, IHC-F

Reactivity: Human, Rat

Conjugate: Biotin

Catalog #1: bsm-43151m-cy3-100ul

Applications: IF(IHC-P)

Reactivity: Human, Rat

Conjugate: Cy3

Catalog #1: bsm-43151m-cy5-100ul

Applications: IF(IHC-P)

Reactivity: Human, Rat

Conjugate: Cy5

Catalog #1: bsm-43151m-Cy5.5-100ul

Applications: IF(IHC-P)

Reactivity: Human, Rat

Conjugate: Cy5.5

Catalog #1: bsm-43151m-cy7-100ul

Applications: IF(IHC-P)

Reactivity: Human, Rat

Conjugate: Cy7

Catalog #1: bsm-43151m-fitc-100ul

Applications: IF(IHC-P)

Reactivity: Human, Rat

Conjugate: FITC

Catalog #1: bsm-43151m-hrp-100ul

Applications: IHC-P, IHC-F

Reactivity: Human, Rat

Conjugate: HRP

Catalog #1: bsm-43151m-pe-100ul

Applications: IF(IHC-P)

Reactivity: Human, Rat

Conjugate: PE

Catalog #1: bsm-43151m-percp-100ul

Applications: IF(IHC-P)

Reactivity: Human, Rat

Conjugate: PerCP

Catalog #1: bsm-43151m-percp-Cy5.5-100ul

Applications: IF(IHC-P)

Reactivity: Human, Rat

Conjugate: PerCP-Cy5.5

Catalog #1: bsm-43151m-percp-cy7-100ul

Applications: IF(IHC-P)

Reactivity: Human, Rat

Conjugate: PerCP-Cy7

Catalog #1: bsm-43151m-rbitc-100ul

Applications: IF(IHC-P)

Reactivity: Human, Rat

Conjugate: RBITC

Catalog #1: bsm-72171m

Applications: WB, ELISA

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-72174m

Applications: ELISA

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-72172m

Applications: WB, ELISA

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-72175m

Applications: ELISA

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-72173m

Applications: WB, ELISA

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-72176m

Applications: ELISA

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-72179m

Applications: WB, ELISA

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-72180m

Applications: WB, ELISA

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-72177m

Applications: ELISA

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-72178m

Applications: WB, ELISA

Reactivity:

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CD68 Monoclonal Antibody, Unconjugated (bsm-60634R) at 1:500 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded (human lung carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (Heme Oxygenase 1) Monoclonal Antibody, Unconjugated (bsm-60751R) at 1:1000 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
25 ug total protein per lane of various lysates (see on figure) probed with Heme Oxygenase 1\/HMOX1 monoclonal antibody, unconjugated (bsm-60751R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Mouse Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Heme Oxygenase 1 Monoclonal Antibody, Unconjugated (bsm-60751R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

Catalog #1: bsm-60751r-100ul

Applications: WB, FCM, IHC-P, IHC-F, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-72181m

Applications: ELISA

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-72183m

Applications: ELISA, IHC

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-72182m

Applications: ELISA

Reactivity:

Conjugate: Unconjugated