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Catalog #1: bsm-60164M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Tissue\/cell: HeLa cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal mouse serum, C-0082) at 37\u00b0C for 20 min; Incubation: Anti-Cyclin B1 Monoclonal Antibody, conjugated (bsm-33042M-AF350) 1:100, 2 hours at 37\u00b0C; PI (red, S0009) was used to stain the cell nuclei.
Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (Histone H3\/HIST3H3 (3G1)) Monoclonal Antibody, Unconjugated (bsm-33042M ) at 1:500 overnight at 4\u00b0C, followed by a conjugated Goat Anti- Mouse IgG antibody (YF488) for 90 minutes, and DAPI for nuclei staining.
Paraformaldehyde-fixed, paraffin embedded (rat testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (Histone H3\/HIST3H3 (3G1)) Monoclonal Antibody, Unconjugated (bsm-33042M ) at 1:500 overnight at 4\u00b0C, followed by a conjugated Goat Anti- Mouse IgG antibody (YF488) for 90 minutes, and DAPI for nuclei staining.
Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (Histone H3\/HIST3H3 (3G1)) Monoclonal Antibody, Unconjugated (bsm-33042M ) at 1:500 overnight at 4\u00b0C, followed by a conjugated Goat Anti- Mouse IgG antibody (YF488) for 90 minutes, and DAPI for nuclei staining.

Catalog #1: bsm-33042m

Applications: WB, IHC-P, IHC-F, IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-33042m-bf350-100ul

Applications: WB, IF

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-350

Catalog #1: bsm-33042m-bf405-100ul

Applications: WB, IF

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-405

Catalog #1: bsm-33042m-bf488-100ul

Applications: WB, IF

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-488

Catalog #1: bsm-33042m-bf555-100ul

Applications: WB, IF

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-555

Catalog #1: bsm-33042m-bf594-100ul

Applications: WB, IF

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-594

Paraformaldehyde-fixed, paraffin embedded Rat Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Incubation: Histone H3\/HIST3H3\/BF647 Antibody, conjugated (bsm-33042M-BF647) at 1:100 overnight at 4\u00b0C; DAPI (blue, C02-04002) was used to stain the cell nuclei.
Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Incubation: Histone H3\/HIST3H3\/BF647 Antibody, conjugated (bsm-33042M-BF647) at 1:100 overnight at 4\u00b0C; DAPI (blue, C02-04002) was used to stain the cell nuclei.
Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Incubation: Histone H3\/HIST3H3\/BF647 Antibody, conjugated (bsm-33042M-BF647) at 1:100 overnight at 4\u00b0C; DAPI (blue, C02-04002) was used to stain the cell nuclei.
Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Incubation: Histone H3\/HIST3H3\/BF647 Antibody, conjugated (bsm-33042M-BF647) at 1:100 overnight at 4\u00b0C; DAPI (blue, C02-04002) was used to stain the cell nuclei.

Catalog #1: bsm-33042m-bf647-100ul

Applications: WB, IF

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-647

Catalog #1: bsm-33042m-bf680-100ul

Applications: WB, IF

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-680

Catalog #1: bsm-33042m-bf750-100ul

Applications: WB, IF

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-750

Catalog #1: bsm-33042m-apc-100ul

Applications: WB, IF

Reactivity: Human, Mouse, Rat

Conjugate: APC

Catalog #1: bsm-33042m-apc-Cy5.5-100ul

Applications: WB, IF

Reactivity: Human, Mouse, Rat

Conjugate: APC-Cy5.5

Catalog #1: bsm-33042m-apc-cy7-100ul

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: APC-Cy7

Catalog #1: bsm-33042m-biotin-100ul

Applications: WB, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: Biotin

Catalog #1: bsm-33042m-cy3-100ul

Applications: WB, IF

Reactivity: Human, Mouse, Rat

Conjugate: Cy3

Catalog #1: bsm-33042m-cy5-100ul

Applications: WB, IF

Reactivity: Human, Mouse, Rat

Conjugate: Cy5

Catalog #1: bsm-33042m-Cy5.5-100ul

Applications: WB, IF

Reactivity: Human, Mouse, Rat

Conjugate: Cy5.5

Catalog #1: bsm-33042m-cy7-100ul

Applications: WB, IF

Reactivity: Human, Mouse, Rat

Conjugate: Cy7

Catalog #1: bsm-33042m-fitc-100ul

Applications: WB, IF

Reactivity: Human, Mouse, Rat

Conjugate: FITC

25 ug total protein per lane of various lysates (see on figure) probed with Histone H3\/HIST3H3\/HRP monoclonal antibody, conjugated (bsm-33042M-HRP) at 1:50000 dilution and 4\u00b0C overnight incubation.
Paraformaldehyde-fixed, paraffin embedded Rat Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Histone H3 Monoclonal Antibody (bsm-33042M-HRP) at 1:200 overnight at 4\u00b0C, and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Histone H3 Monoclonal Antibody (bsm-33042M-HRP) at 1:200 overnight at 4\u00b0C, and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Histone H3 Monoclonal Antibody (bsm-33042M-HRP) at 1:200 overnight at 4\u00b0C, and DAB (C-0010) staining.

Catalog #1: bsm-33042m-hrp-100ul

Applications: WB, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: HRP

Catalog #1: bsm-33042m-pe-100ul

Applications: WB, IF

Reactivity: Human, Mouse, Rat

Conjugate: PE

Catalog #1: bsm-33042m-pe-cy5-100ul

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: PE-Cy5

Catalog #1: bsm-33042m-pe-Cy5.5-100ul

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: PE-Cy5.5

Catalog #1: bsm-33042m-pe-cy7-100ul

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: PE-Cy7

Catalog #1: bsm-33042m-percp-100ul

Applications: WB, IF

Reactivity: Human, Mouse, Rat

Conjugate: PerCP

Catalog #1: bsm-33042m-percp-Cy5.5-100ul

Applications: WB, IF

Reactivity: Human, Mouse, Rat

Conjugate: PerCP-Cy5.5

Catalog #1: bsm-33042m-percp-cy7-100ul

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: PerCP-Cy7

Catalog #1: bsm-33042m-rbitc-100ul

Applications: WB, IF

Reactivity: Human, Mouse, Rat

Conjugate: RBITC

Paraformaldehyde-fixed, paraffin embedded Rat pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Histone H3(di methyl K27) Monoclonal Antibody, Unconjugated (bsm-33107M) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human breast; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Histone H3(di methyl K27) Monoclonal Antibody, Unconjugated (bsm-33107M) at 1:200 overnight at 4\u00b0C, DAB staining.
25 ug total protein per lane of various lysates (see on figure) probed with Histone H3(di methyl K27) monoclonal antibody, unconjugated (bsm-33107M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Rat Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Histone H3(di methyl K27) Monoclonal Antibody, Unconjugated (bsm-33107M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.

Catalog #1: bsm-33107m

Applications: WB, IHC-P, IHC-F, IF(ICC), IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-33107m-bf350-100ul

Applications: WB, IF(ICC), IF

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-350

Catalog #1: bsm-33107m-bf405-100ul

Applications: WB, IF(ICC), IF

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-405

Catalog #1: bsm-33107m-bf488-100ul

Applications: WB, IF(ICC), IF

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-488

Catalog #1: bsm-33107m-bf555-100ul

Applications: WB, IF(ICC), IF

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-555

Catalog #1: bsm-33107m-bf594-100ul

Applications: WB, IF(ICC), IF

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-594

Catalog #1: bsm-33107m-bf647-100ul

Applications: WB, IF(ICC), IF

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-647

Catalog #1: bsm-33107m-bf680-100ul

Applications: WB, IF(ICC), IF

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-680

Catalog #1: bsm-33107m-bf750-100ul

Applications: WB, IF(ICC), IF

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-750

Catalog #1: bsm-33107m-apc-100ul

Applications: WB, IF(ICC), IF

Reactivity: Human, Mouse, Rat

Conjugate: APC

Catalog #1: bsm-33107m-apc-Cy5.5-100ul

Applications: WB, IF(ICC), IF

Reactivity: Human, Mouse, Rat

Conjugate: APC-Cy5.5

Catalog #1: bsm-33107m-apc-cy7-100ul

Applications: WB, IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: APC-Cy7

Catalog #1: bsm-33107m-biotin-100ul

Applications: WB, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: Biotin

Catalog #1: bsm-33107m-cy3-100ul

Applications: WB, IF(ICC), IF

Reactivity: Human, Mouse, Rat

Conjugate: Cy3

Catalog #1: bsm-33107m-cy5-100ul

Applications: WB, IF(ICC), IF

Reactivity: Human, Mouse, Rat

Conjugate: Cy5

Catalog #1: bsm-33107m-Cy5.5-100ul

Applications: WB, IF(ICC), IF

Reactivity: Human, Mouse, Rat

Conjugate: Cy5.5

Catalog #1: bsm-33107m-cy7-100ul

Applications: WB, IF(ICC), IF

Reactivity: Human, Mouse, Rat

Conjugate: Cy7

Catalog #1: bsm-33107m-fitc-100ul

Applications: WB, IF(ICC), IF

Reactivity: Human, Mouse, Rat

Conjugate: FITC

Catalog #1: bsm-33107m-hrp-100ul

Applications: WB, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: HRP

Catalog #1: bsm-33107m-pe-100ul

Applications: WB, IF(ICC), IF

Reactivity: Human, Mouse, Rat

Conjugate: PE