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Catalog #1: bsm-36102m-Cy5.5-100ul

Applications: WB

Reactivity: Human

Conjugate: Cy5.5

Catalog #1: bsm-10697m-cy7-100ul

Applications: WB, IF

Reactivity: Human

Conjugate: Cy7

Catalog #1: bsm-36102m-cy7-100ul

Applications: WB

Reactivity: Human

Conjugate: Cy7

Catalog #1: bsm-10697m-fitc-100ul

Applications: WB, IF

Reactivity: Human

Conjugate: FITC

Catalog #1: bsm-36102m-fitc-100ul

Applications: WB

Reactivity: Human

Conjugate: FITC

Catalog #1: bsm-10697m-hrp-100ul

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human

Conjugate: HRP

Catalog #1: bsm-36102m-hrp-100ul

Applications: WB, ELISA

Reactivity: Human

Conjugate: HRP

Catalog #1: bsm-10697m-pe-100ul

Applications: WB, IF

Reactivity: Human

Conjugate: PE

Catalog #1: bsm-36102m-pe-100ul

Applications: WB

Reactivity: Human

Conjugate: PE

Catalog #1: bsm-10697m-pe-cy5-100ul

Applications: WB

Reactivity: Human

Conjugate: PE-Cy5

Catalog #1: bsm-36102m-pe-cy5-100ul

Applications: WB

Reactivity: Human

Conjugate: PE-Cy5

Catalog #1: bsm-10697m-pe-Cy5.5-100ul

Applications: WB

Reactivity: Human

Conjugate: PE-Cy5.5

Catalog #1: bsm-36102m-pe-Cy5.5-100ul

Applications: WB

Reactivity: Human

Conjugate: PE-Cy5.5

Catalog #1: bsm-10697m-pe-cy7-100ul

Applications: WB

Reactivity: Human

Conjugate: PE-Cy7

Catalog #1: bsm-36102m-pe-cy7-100ul

Applications: WB

Reactivity: Human

Conjugate: PE-Cy7

Catalog #1: bsm-10697m-percp-100ul

Applications: WB, IF

Reactivity: Human

Conjugate: PerCP

Catalog #1: bsm-36102m-percp-100ul

Applications: WB

Reactivity: Human

Conjugate: PerCP

Catalog #1: bsm-10697m-percp-Cy5.5-100ul

Applications: WB, IF

Reactivity: Human

Conjugate: PerCP-Cy5.5

Catalog #1: bsm-36102m-percp-Cy5.5-100ul

Applications: WB

Reactivity: Human

Conjugate: PerCP-Cy5.5

Catalog #1: bsm-36102m-percp-cy7-100ul

Applications: WB

Reactivity: Human

Conjugate: PerCP-Cy7

Catalog #1: bsm-10697m-percp-cy7-100ul

Applications: WB

Reactivity: Human

Conjugate: PerCP-Cy7

Catalog #1: bsm-10697m-rbitc-100ul

Applications: WB, IF

Reactivity: Human

Conjugate: RBITC

Catalog #1: bsm-36102m-rbitc-100ul

Applications: WB

Reactivity: Human

Conjugate: RBITC

HepG2 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (IDE) polyclonal Antibody, Unconjugated (bs-0018M) 1:25, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Mouse IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
HepG2 cells were\u00a0fixed with 4% PFA for 10min at room temperature,permeabilized with\u00a090% ice-cold methanol for 20 min at -20\u2103,\u00a0and incubated in 5% BSA blocking buffer\u00a0for 30 min at room temperature. Cells were then stained with IDE Antibody(bs-0018M)at 1:50 dilution\u00a0in blocking buffer and\u00a0incubated for 30 min at\u00a0room temperature,\u00a0washed twice with 2%BSA in PBS,\u00a0followed by\u00a0secondary antibody incubation\u00a0for 40 min\u00a0at\u00a0room temperature. Acquisitions of 20,000 events were performed.\u00a0Cells stained with primary antibody\u00a0(green), and isotype control (orange).

Catalog #1: bs-0018m

Applications: IHC-P, IHC-F, IF, Flow-Cyt, ICC/IF

Reactivity: Mouse

Conjugate: Unconjugated

Catalog #1: bsm-60556m

Applications: WB, IHC-P, IHC-F, IF, ICC/IF

Reactivity: Human, Rat

Conjugate: Unconjugated

Lane 1: HeLa Cell lysates; Lane 2: MCF-7 Cell lysates; Lane 3: K562 Cell lysates; Lane 4: NIH\/3T3 Cell lysates; Lane 5: PC-12 Cell lysates; probed with IF4E (4C5) Monoclonal Antibody, unconjugated (bsm-51305M) at 1:4000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized MCF-7 cells labeled with IF4E (4C5) Monoclonal Antibody (bsm-51305M) at 1\/25 dilution, followed by secondary antibody (green), cytoplasmic actin staining (red), and nuclear counter stain with DAPI (blue).

Catalog #1: bsm-51305M

Applications: WB, IF(ICC), IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded (human spleen); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (IFI16) Monoclonal Antibody, Unconjugated (bsm-60844M) at 1:300 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human tonsil); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (IFI16) Monoclonal Antibody, Unconjugated (bsm-60844M) at 1:300 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (IFI16) Monoclonal Antibody, Unconjugated (bsm-60844M) at 1:300 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human tonsils; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with IFI16 Monoclonal Antibody, Unconjugated (bsm-60844M) at 1:200 overnight at 4\u00b0C. Followed by conjugated Goat Anti-Mouse IgG antibody (red, bs-0296G-BF594), DAPI (blue, C02-04002) was used to stain the cell nuclei.

Catalog #1: bsm-60844m-100ul

Applications: IHC-P, IHC-F, IF(IHC-P)

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-0388M-BF647

Applications: WB, IF(IHC-P)

Reactivity: Human

Conjugate: AbBy Fluor® 647

Catalog #1: bsm-0388M-BF680

Applications: WB, IF(IHC-P)

Reactivity: Human

Conjugate: AbBy Fluor® 680

Catalog #1: bsm-0388M-Biotin

Applications: WB, IHC-P

Reactivity: Human

Conjugate: Biotin

Catalog #1: bsm-0388M-HRP

Applications: WB, IHC-P

Reactivity: Human

Conjugate: HRP

The Jurkat\uff08Treated with PMA (25 ng\/mL, 6 h) and ionomycin (1 \u00b5g\/mL, 6 h), BFA (5 \u00b5g\/ml, last 5 h)\uff09 (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.).Primary Antibody (green):Mouse Anti-IFN gamma antibody (bsm-0388M): 1 \u03bcg\/10^6 cells; Secondary Antibody (white blue): Goat anti-Mouse IgG-BF488 (bs-60296G-BF488): 1 \u03bcg\/test.Blank control (black): PBS. Acquisition of 20,000 events was performed.\r\n
4% Paraformaldehyde-fixed Jurkat\uff08Treated with PMA (25 ng\/mL, 6 h) and ionomycin (1 \u00b5g\/mL, 6 h), BFA (5 \u00b5g\/ml, last 5 h)\uff09(H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (IFN gamma) monoclonal Antibody, unconjugated (bsm-0388M) 1:100, 90 min at 37\u00b0C; followed by conjugated Goat Anti-Mouse IgG antibody (green, bs-60296G-BF488) at 37\u00b0C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.

Catalog #1: bsm-0388m

Applications: Flow-Cyt, ICC/IF

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-0388m-bf350-100ul

Applications: Flow-Cyt

Reactivity: Human

Conjugate: AbBy Fluor-350

Catalog #1: bsm-0388m-bf405-100ul

Applications: Flow-Cyt

Reactivity: Human

Conjugate: AbBy Fluor-405

Catalog #1: bsm-0388m-bf488-100ul

Applications: Flow-Cyt

Reactivity: Human

Conjugate: AbBy Fluor-488

Catalog #1: bsm-0388m-bf555-100ul

Applications: Flow-Cyt

Reactivity: Human

Conjugate: AbBy Fluor-555

Catalog #1: bsm-0388m-bf594-100ul

Applications: Flow-Cyt

Reactivity: Human

Conjugate: AbBy Fluor-594

Catalog #1: bsm-0388m-bf750-100ul

Applications: Flow-Cyt

Reactivity: Human

Conjugate: AbBy Fluor-750

Catalog #1: bsm-0388m-apc-100ul

Applications: Flow-Cyt

Reactivity: Human

Conjugate: APC

Catalog #1: bsm-0388m-apc-Cy5.5-100ul

Applications: Flow-Cyt

Reactivity: Human

Conjugate: APC-Cy5.5

Catalog #1: bsm-0388m-apc-cy7-100ul

Applications: Flow-Cyt

Reactivity: Human

Conjugate: APC-Cy7

Catalog #1: bsm-0388m-cy3-100ul

Applications: Flow-Cyt

Reactivity: Human

Conjugate: Cy3

Catalog #1: bsm-0388m-cy5-100ul

Applications: Flow-Cyt

Reactivity: Human

Conjugate: Cy5

Catalog #1: bsm-0388m-Cy5.5-100ul

Applications: Flow-Cyt

Reactivity: Human

Conjugate: Cy5.5

Catalog #1: bsm-0388m-cy7-100ul

Applications: Flow-Cyt

Reactivity: Human

Conjugate: Cy7

Catalog #1: bsm-0388m-fitc-100ul

Applications: Flow-Cyt

Reactivity: Human

Conjugate: FITC

Catalog #1: bsm-0388m-pe-100ul

Applications: Flow-Cyt

Reactivity: Human

Conjugate: PE

Catalog #1: bsm-0388m-pe-cy5-100ul

Applications: Flow-Cyt

Reactivity: Human

Conjugate: PE-Cy5