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Catalog #1: bsm-0855m-percp-cy7-100ul

Applications: IF(IHC-P), IF(ICC)

Reactivity: Human

Conjugate: PerCP-Cy7

Catalog #1: bsm-0855m-rbitc-100ul

Applications: IF(IHC-P), IF(ICC)

Reactivity: Human

Conjugate: RBITC

Blocking buffer: 5% NFDM\/TBST\r\nPrimary ab dilution: 1:1000\r\nPrimary ab incubation condition: 4\u00b0C overnight\r\nLysate: HeLa, C2C12\r\nProtein loading quantity: 20 \u03bcg\r\nExposure time: 30 s\r\nPredicted MW: 120 kDa\r\nObserved MW: 130 kDa
Blocking buffer: 5% NFDM\/TBST\r\nPrimary ab dilution: 1:5000\r\nPrimary ab incubation condition: 4\u00b0C overnight\r\nLysate: A549\r\nProtein loading quantity: 20 \u03bcg\r\nExposure time: 30 s\r\nPredicted MW: 120 kDa\r\nObserved MW: 130 kDa
Tissue: Human lung adenocarcinoma\r\nSection type: Formalin fixed & Paraffin -embedded section\r\nRetrieval method: High temperature and high pressure\r\nRetrieval buffer: Tris\/EDTA buffer, pH 9.0 Primary ab dilution: 1:100\r\nPrimary ab incubation condition: 1 hour at room temperature\r\nCounter stain: Hematoxylin\r\nComment: Color brown is the positive signal for bsm-60363M

Catalog #1: bsm-60363m

Applications: WB, IHC-P, IHC-F, IF

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bsm-70525M

Applications: WB, IP

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-70526M

Applications: WB, IP

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-70527M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-70528M

Applications: WB, IP

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-70524M

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-43005M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-43005M-BF350

Applications: WB

Reactivity: Human

Conjugate: AbBy Fluor-350

Catalog #1: bsm-43005M-BF405

Applications: WB

Reactivity: Human

Conjugate: AbBy Fluor-405

Catalog #1: bsm-43005M-BF488

Applications: WB

Reactivity: Human

Conjugate: AbBy Fluor-488

Catalog #1: bsm-43005M-BF555

Applications: WB

Reactivity: Human

Conjugate: AbBy Fluor-555

Catalog #1: bsm-43005M-BF594

Applications: WB

Reactivity: Human

Conjugate: AbBy Fluor-594

Catalog #1: bsm-43005M-BF647

Applications: WB

Reactivity: Human

Conjugate: AbBy Fluor-647

Catalog #1: bsm-43005M-BF680

Applications: WB

Reactivity: Human

Conjugate: AbBy Fluor-680

Catalog #1: bsm-43005M-BF750

Applications: WB

Reactivity: Human

Conjugate: AbBy Fluor-750

Catalog #1: bsm-43005M-APC

Applications: WB

Reactivity: Human

Conjugate: APC

Catalog #1: bsm-43005M-APC-Cy5.5

Applications: WB

Reactivity: Human

Conjugate: APC-Cy5.5

Catalog #1: bsm-43005M-APC-Cy7

Applications: WB

Reactivity: Human

Conjugate: APC-Cy7

Catalog #1: bsm-43005M-Biotin

Applications: WB

Reactivity: Human

Conjugate: Biotin

Catalog #1: bsm-43005M-Cy3

Applications: WB

Reactivity: Human

Conjugate: Cy3

Catalog #1: bsm-43005M-Cy5

Applications: WB

Reactivity: Human

Conjugate: Cy5

Catalog #1: bsm-43005M-Cy5.5

Applications: WB

Reactivity: Human

Conjugate: Cy5.5

Catalog #1: bsm-43005M-Cy7

Applications: WB

Reactivity: Human

Conjugate: Cy7

Catalog #1: bsm-43005M-FITC

Applications: WB

Reactivity: Human

Conjugate: FITC

Catalog #1: bsm-43005M-HRP

Applications: WB

Reactivity: Human

Conjugate: HRP

Catalog #1: bsm-43005M-PE

Applications: WB

Reactivity: Human

Conjugate: PE

Catalog #1: bsm-43005M-PE-Cy5

Applications: WB

Reactivity: Human

Conjugate: PE-Cy5

Catalog #1: bsm-43005M-PE-Cy5.5

Applications: WB

Reactivity: Human

Conjugate: PE-Cy5.5

Catalog #1: bsm-43005M-PE-Cy7

Applications: WB

Reactivity: Human

Conjugate: PE-Cy7

Catalog #1: bsm-43005M-PerCP

Applications: WB

Reactivity: Human

Conjugate: PerCP

Catalog #1: bsm-43005M-PerCP-Cy5.5

Applications: WB

Reactivity: Human

Conjugate: PerCP-Cy5.5

Catalog #1: bsm-43005M-RBITC

Applications: WB

Reactivity: Human

Conjugate: RBITC

Blocking buffer: 5% NFDM\/TBST\r\nPrimary Ab dilution: 1:1000\r\nPrimary Ab incubation condition: 4\u00b0C overnight\r\nSecondary Ab: Goat Anti-Mouse IgG H&L (HRP)\r\nLysate: 1: HeLa, 2: HEK-293, 3: MCF7, 4: RAW 264.7\r\nProtein loading quantity: 20 \u03bcg\r\nExposure time: 60 s\r\nPredicted MW: 118 kDa\r\nObserved MW: 118 kDa
Blocking buffer: 5% NFDM\/TBST\r\nPrimary ab dilution: 1:1000\r\nPrimary ab incubation condition: lane 1: PTM-5729+competitive peptide, lane 2: PTM-5729, 2 hours at room temperature\r\nSecondary ab: Goat Anti-Rabbit IgG H&L (HRP)\r\nLysate: C6\r\nProtein loading quantity: 20 \u03bcg\r\nExposure time: 60 s\r\nPredicted MW: 19 kDa\r\nObserved MW: 19 kDa

Catalog #1: bsm-60436M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa cells labeled with IRF3 (2C4) Monoclonal Antibody (bsm-51312M) at 1\/25 dilution, followed by secondary antibody (green), cytoplasmic actin staining (red), and nuclear counter stain with DAPI (blue).
Jurkat cells probed with using IRF3 (2C4) Monoclonal Antibody bsm-51312M at 1:25 for 60 minutes at 37\u00baC followed by secondary antibody at 1:400 (green) compared to an isotype control of mouse IgG (blue).
Lane 1: COS-7; Lane 2: Daudi; Lane 3: HepG2; Lane 4: HT-29; Lane 5: Jurkat; Lane 6: MCF-7; probed with IRF3 (2C4) Polyclonal Antibody, unconjugated (bsm-51312M) at 1:2000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Paraformaldehyde-fixed, paraffin embedded human kidney tissue; Blocked with 3% BSA for 30min at RT; Antibody incubation with IRF3 (2C4) Monoclonal Antibody, Unconjugated (bsm-51312M) at 1:25 for 1hr at 37\u00b0C, followed by a conjugated secondary antibody and DAB staining.

Catalog #1: bsm-51312M

Applications: WB, FCM, IHC-P, IF(ICC), IF

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-56211m

Applications: IHC

Reactivity: Human

Conjugate: Unconjugated

Lane 1: Mouse brain tissue lysates probed with Irgm1 Monoclonal Antibody, Unconjugated (bsm-51508M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Mouse brain tissue lysates; Lane 2: Mouse Heart tissue lysates probed with Irgm1 Monoclonal Antibody, Unconjugated (bsm-51508M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bsm-51508m

Applications: WB

Reactivity: Mouse

Conjugate: Unconjugated

Catalog #1: bsm-51373m-100ul

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51027M

Applications: WB, FCM, IHC-P, IF

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51661m

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51250M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Lane 1: HL-60; Lane 2: K562 cell lysate at 20 \u00b5g per lane, probed with bsm-51424M ITGB2 (534CT6.2.1) Monoclonal Antibody at 1:1000 dilution and 4\u2103 overnight incubation, followed by secondary antibody incubation for 60min at room temperature.
Paraformaldehyde-fixed, paraffin embedded human tonsil tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 15 minutes; Blocking buffer (3% BSA) at room temperature for 30min; Antibody incubation with ITGB2 (534CT6.2.1) Monoclonal Antibody (bsm-51424M) at 1:25 for 1 hour at 37\u00b0C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.

Catalog #1: bsm-51424M

Applications: WB, IHC-P

Reactivity: Human

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Human liver cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with \r\nJAK1 Monoclonal Antibody, Unconjugated (bsm-33268M) at 1:400 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human brain glioma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with \r\nJAK1 Monoclonal Antibody, Unconjugated (bsm-33268M) at 1:400 overnight at 4\u00b0C, DAB staining.
MCF-7 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20\u2103, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with JAK1 Monoclonal Antibody(bsm-33267M)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

Catalog #1: bsm-33268M

Applications: IHC-P, IHC-F, IF(ICC), IF

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-33268M-BF350

Applications: IF(ICC), IF

Reactivity: Human

Conjugate: AbBy Fluor-350

Catalog #1: bsm-33268M-BF405

Applications: IF(ICC), IF

Reactivity: Human

Conjugate: AbBy Fluor-405

Catalog #1: bsm-33268M-BF488

Applications: IF(ICC), IF

Reactivity: Human

Conjugate: AbBy Fluor-488

Catalog #1: bsm-33268M-BF555

Applications: IF(ICC), IF

Reactivity: Human

Conjugate: AbBy Fluor-555