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Catalog #1: bsm-33442M-PE

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: PE

Catalog #1: bsm-33040m-pe-cy5-100ul

Applications: WB

Reactivity: Human

Conjugate: PE-Cy5

Catalog #1: bsm-33442M-PE-Cy5

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: PE-Cy5

Catalog #1: bsm-33040m-pe-Cy5.5-100ul

Applications: WB

Reactivity: Human

Conjugate: PE-Cy5.5

Catalog #1: bsm-33442M-PE-Cy5.5

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: PE-Cy5.5

Catalog #1: bsm-33040m-pe-cy7-100ul

Applications: WB

Reactivity: Human

Conjugate: PE-Cy7

Catalog #1: bsm-33442M-PE-Cy7

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: PE-Cy7

Catalog #1: bsm-33040m-percp-100ul

Applications: WB, IF

Reactivity: Human

Conjugate: PerCP

Catalog #1: bsm-33442M-PerCP

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: PerCP

Catalog #1: bsm-33040m-percp-Cy5.5-100ul

Applications: WB, IF

Reactivity: Human

Conjugate: PerCP-Cy5.5

Catalog #1: bsm-33442M-PerCP-Cy5.5

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: PerCP-Cy5.5

Catalog #1: bsm-33040m-percp-cy7-100ul

Applications: WB

Reactivity: Human

Conjugate: PerCP-Cy7

Catalog #1: bsm-33040m-rbitc-100ul

Applications: WB, IF

Reactivity: Human

Conjugate: RBITC

Catalog #1: bsm-33442M-RBITC

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: RBITC

Catalog #1: bsm-70537M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-70139M

Applications: WB, IHC

Reactivity: Human

Conjugate: Unconjugated

4% Paraformaldehyde-fixed Hela (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (LAMP1) monoclonal Antibody, unconjugated (bsm-51301M) 1:100, 90 min at 37\u00b0C; followed by conjugated Goat Anti-Mouse IgG antibody (green, bs-60296G-FITC) at 37\u00b0C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.
The Hela (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.).Primary Antibody (green):Mouse Anti-LAMP1 antibody (bsm-51301M): 1 \u03bcg\/10^6 cells; Secondary Antibody (white blue): Goat anti-Mouse IgG-BF488 (bs-60296G-BF488): 1 \u03bcg\/test. Blank control (black): PBS. Acquisition of 20,000 events was performed.
The HT-1080 (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.).Primary Antibody (green):Mouse Anti-LAMP1 antibody (bsm-51030M): 1 \u03bcg\/10^6 cells; Secondary Antibody (white blue): Goat anti-Mouse IgG-BF488 (bs-60296G-BF488): 1 \u03bcg\/test. Blank control (black): PBS. Acquisition of 20,000 events was performed.
25 ug total protein per lane of various lysates (see on figure) probed with LAMP1 monoclonal antibody, unconjugated (bsm-51301M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Catalog #1: bsm-51301m

Applications: WB, IHC-P, IF

Reactivity: Human

Conjugate: Unconjugated

Blocking buffer: 5% NFDM\/TBST\r\nPrimary ab dilution: 1:1000\r\nPrimary ab incubation condition: 2 hours at room temperature\r\nSecondary ab: Goat Anti-Rabbit IgG H&L (HRP)\r\nLysate: HeLa, JAR\r\nProtein loading quantity: 20 \u03bcg\r\nExposure time: 3 s\r\nPredicted MW: 45 kDa\r\nObserved MW: 110 kDa\r\n
Lane 1: Human HT-1080 cell Lysates; Lane 2: Human JAR cell Lysates; Lane 3: Human A549 cell Lysates; Lane 4: Human U-87 MG cell Lysates. Probed with LAMP1 monoclonal Antibody, unconjugated (bsm-60009M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Cell line: HeLa\r\nFixative: 100% Ice-cold methanol\r\nPermeabilization: 0.1% TritonX-100\r\nPrimary ab dilution: 1:50\r\nPrimary incubation condition: 4\u00b0C overnight\r\nSecondary ab: Goat Anti-Rabbit IgG\r\nNuclear counter stain: DAPI (Blue)\r\nComment: Color green is the positive signal for bsm-60009M

Catalog #1: bsm-60009M

Applications: WB, ELISA

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51301m-bf350-100ul

Applications: WB, IF

Reactivity: Human

Conjugate: AbBy Fluor-350

Catalog #1: bsm-51301m-bf405-100ul

Applications: WB, IF

Reactivity: Human

Conjugate: AbBy Fluor-405

Catalog #1: bsm-51301m-bf488-100ul

Applications: WB, IF

Reactivity: Human

Conjugate: AbBy Fluor-488

Catalog #1: bsm-51301m-bf555-100ul

Applications: WB, IF

Reactivity: Human

Conjugate: AbBy Fluor-555

Catalog #1: bsm-51301m-bf594-100ul

Applications: WB, IF

Reactivity: Human

Conjugate: AbBy Fluor-594

Catalog #1: bsm-51301m-bf647-100ul

Applications: WB, IF

Reactivity: Human

Conjugate: AbBy Fluor-647

Catalog #1: bsm-51301m-bf680-100ul

Applications: WB, IF

Reactivity: Human

Conjugate: AbBy Fluor-680

Catalog #1: bsm-51301m-bf750-100ul

Applications: WB, IF

Reactivity: Human

Conjugate: AbBy Fluor-750

Catalog #1: bsm-51301m-apc-100ul

Applications: WB, IF

Reactivity: Human

Conjugate: APC

Catalog #1: bsm-51301m-apc-Cy5.5-100ul

Applications: WB, IF

Reactivity: Human

Conjugate: APC-Cy5.5

Catalog #1: bsm-51301m-apc-cy7-100ul

Applications: WB

Reactivity: Human

Conjugate: APC-Cy7

Catalog #1: bsm-51301m-biotin-100ul

Applications: WB, IHC-P

Reactivity: Human

Conjugate: Biotin

Catalog #1: bsm-51301m-cy3-100ul

Applications: WB, IF

Reactivity: Human

Conjugate: Cy3

Catalog #1: bsm-51301m-cy5-100ul

Applications: WB, IF

Reactivity: Human

Conjugate: Cy5

Catalog #1: bsm-51301m-Cy5.5-100ul

Applications: WB, IF

Reactivity: Human

Conjugate: Cy5.5

Catalog #1: bsm-51301m-cy7-100ul

Applications: WB, IF

Reactivity: Human

Conjugate: Cy7

Catalog #1: bsm-51301m-fitc-100ul

Applications: WB, IF

Reactivity: Human

Conjugate: FITC

Catalog #1: bsm-51301m-hrp-100ul

Applications: WB, IHC-P

Reactivity: Human

Conjugate: HRP

Catalog #1: bsm-51301m-pe-100ul

Applications: WB, IF

Reactivity: Human

Conjugate: PE

Catalog #1: bsm-51301m-pe-cy5-100ul

Applications: WB

Reactivity: Human

Conjugate: PE-Cy5

Catalog #1: bsm-51301m-pe-Cy5.5-100ul

Applications: WB

Reactivity: Human

Conjugate: PE-Cy5.5

Catalog #1: bsm-51301m-pe-cy7-100ul

Applications: WB

Reactivity: Human

Conjugate: PE-Cy7

Catalog #1: bsm-51301m-percp-100ul

Applications: WB, IF

Reactivity: Human

Conjugate: PerCP

Catalog #1: bsm-51301m-percp-Cy5.5-100ul

Applications: WB, IF

Reactivity: Human

Conjugate: PerCP-Cy5.5

Catalog #1: bsm-51301m-percp-cy7-100ul

Applications: WB

Reactivity: Human

Conjugate: PerCP-Cy7

Catalog #1: bsm-51301m-rbitc-100ul

Applications: WB, IF

Reactivity: Human

Conjugate: RBITC

Catalog #1: bsm-51034M

Applications: WB, IHC-P

Reactivity: Human, Mouse

Conjugate: Unconjugated

25 ug total protein per lane of various lysates (see on figure) probed with LAMP2 monoclonal antibody, unconjugated (bsm-51474M) at 1:500 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Human Liver Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with LAMP2 Monoclonal Antibody, Unconjugated (bsm-51474M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with LAMP2 Monoclonal Antibody, Unconjugated (bsm-51474M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Thyroid Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with LAMP2 Monoclonal Antibody, Unconjugated (bsm-51474M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.

Catalog #1: bsm-51474m-100ul

Applications: WB, IHC-P, IHC-F, IF(IHC-P)

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-0940M

Applications: WB, ELISA, IHC-P, IHC-F, IF

Reactivity: Human, Rat

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with LC3A Monoclonal Antibody, Unconjugated (bsm-33309M) at 1:400 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with LC3A Monoclonal Antibody, Unconjugated (bsm-33309M) at 1:400 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (LC3A ) Monoclonal Antibody, Unconjugated (bsm-33309M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (LC3A ) Monoclonal Antibody, Unconjugated (bsm-33309M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.

Catalog #1: bsm-33309m

Applications: WB, IHC-P, IHC-F, IF(ICC), IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated