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Catalog #1: bsm-33080m-apc-cy7-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: APC-Cy7

Catalog #1: bsm-33080m-biotin-100ul

Applications: WB, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: Biotin

Catalog #1: bsm-33080m-cy3-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: Cy3

Catalog #1: bsm-33080m-cy5-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: Cy5

Catalog #1: bsm-33080m-Cy5.5-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: Cy5.5

Catalog #1: bsm-33080m-cy7-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: Cy7

Catalog #1: bsm-33080m-fitc-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: FITC

Catalog #1: bsm-33080m-hrp-100ul

Applications: WB, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: HRP

Catalog #1: bsm-33080m-pe-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: PE

Catalog #1: bsm-33080m-pe-cy5-100ul

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: PE-Cy5

Catalog #1: bsm-33080m-pe-Cy5.5-100ul

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: PE-Cy5.5

Catalog #1: bsm-33080m-pe-cy7-100ul

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: PE-Cy7

Catalog #1: bsm-33080m-percp-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: PerCP

Catalog #1: bsm-33080m-percp-Cy5.5-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: PerCP-Cy5.5

Catalog #1: bsm-33080m-percp-cy7-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: PerCP-Cy7

Catalog #1: bsm-33080m-rbitc-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: RBITC

Catalog #1: bsm-51296M

Applications: WB, IHC-P

Reactivity: Human, Mouse

Conjugate: Unconjugated

Lane 1: 293 Cell lysates; Lane 2: K562 Cell lysates; Lane 3: MCF-7 Cell lysates; Lane 4: SK-BR-3 Cell lysates; Lane 5: T47D Cell lysates; Lane 6: MDA-MB-453 Cell lysates; Lane 7: mouse testis lysates; probed with ACE2 (881CT16.4.4) Monoclonal Antibody, unconjugated (bsm-51221M) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Paraformaldehyde-fixed, paraffin embedded (Human kidney ); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (ACE2(1C4) ) Monoclonal Antibody, Unconjugated (bsm-51221M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Human kidney cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (ACE2(1C4) ) Monoclonal Antibody, Unconjugated (bsm-51221M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Human esophageal cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (ACE2(1C4) ) Monoclonal Antibody, Unconjugated (bsm-51221M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.

Catalog #1: bsm-51221M

Applications: WB, ELISA, IHC-P, IHC-F, IF, ICC/IF

Reactivity: Human

Conjugate: Unconjugated

The Hela (Treated Sodium Butyrate (5mM, 24h))(H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.).Primary Antibody (green):Mouse Anti-Acetyl Lysine antibody (bsm-33202M): 1 \u03bcg\/10^6 cells; Secondary Antibody (white blue): Goat anti-Mouse IgG-BF488 (bs-60296G-BF488): 1 \u03bcg\/test. Blank control (black): PBS. Acquisition of 20,000 events was performed.
4% Paraformaldehyde-fixed Hela\uff08Treated Sodium Butyrate (5mM, 24h)\uff09 (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (Acetyl Lysine) monoclonal Antibody, unconjugated (bsm-33202M) 1:100, 90 min at 37\u00b0C; followed by conjugated Goat Anti-Mouse IgG antibody (green, bs-60296G-BF488) at 37\u00b0C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.

Catalog #1: bsm-33202m-100ul

Applications: FCM, IHC-P, IHC-F, IF(IHC-P), IF(ICC)

Reactivity: Species independent

Conjugate: Unconjugated

Catalog #1: bsm-33202m-bf350-100ul

Applications: FCM, IF(IHC-P), IF(ICC)

Reactivity: Species independent

Conjugate: AbBy Fluor-350

Catalog #1: bsm-33202m-bf405-100ul

Applications: FCM, IF(IHC-P), IF(ICC)

Reactivity: Species independent

Conjugate: AbBy Fluor-405

Catalog #1: bsm-33202m-bf488-100ul

Applications: FCM, IF(IHC-P), IF(ICC)

Reactivity: Species independent

Conjugate: AbBy Fluor-488

Catalog #1: bsm-33202m-bf555-100ul

Applications: FCM, IF(IHC-P), IF(ICC)

Reactivity: Species independent

Conjugate: AbBy Fluor-555

Catalog #1: bsm-33202m-bf594-100ul

Applications: FCM, IF(IHC-P), IF(ICC)

Reactivity: Species independent

Conjugate: AbBy Fluor-594

Catalog #1: bsm-33202m-bf647-100ul

Applications: FCM, IF(IHC-P), IF(ICC)

Reactivity: Species independent

Conjugate: AbBy Fluor-647

Catalog #1: bsm-33202m-bf680-100ul

Applications: FCM, IF(IHC-P), IF(ICC)

Reactivity: Species independent

Conjugate: AbBy Fluor-680

Catalog #1: bsm-33202m-bf750-100ul

Applications: FCM, IF(IHC-P), IF(ICC)

Reactivity: Species independent

Conjugate: AbBy Fluor-750

Catalog #1: bsm-33202m-apc-100ul

Applications: FCM, IF(IHC-P), IF(ICC)

Reactivity: Species independent

Conjugate: APC

Catalog #1: bsm-33202m-apc-Cy5.5-100ul

Applications: FCM, IF(IHC-P), IF(ICC)

Reactivity: Species independent

Conjugate: APC-Cy5.5

Catalog #1: bsm-33202m-apc-cy7-100ul

Applications: FCM, IF(IHC-P), IF(ICC)

Reactivity: Species independent

Conjugate: APC-Cy7

Catalog #1: bsm-33202m-biotin-100ul

Applications: IHC-P, IHC-F

Reactivity: Species independent

Conjugate: Biotin

Catalog #1: bsm-33202m-cy3-100ul

Applications: FCM, IF(IHC-P), IF(ICC)

Reactivity: Species independent

Conjugate: Cy3

Catalog #1: bsm-33202m-cy5-100ul

Applications: FCM, IF(IHC-P), IF(ICC)

Reactivity: Species independent

Conjugate: Cy5

Catalog #1: bsm-33202m-Cy5.5-100ul

Applications: FCM, IF(IHC-P), IF(ICC)

Reactivity: Species independent

Conjugate: Cy5.5

Catalog #1: bsm-33202m-cy7-100ul

Applications: FCM, IF(IHC-P), IF(ICC)

Reactivity: Species independent

Conjugate: Cy7

Catalog #1: bsm-33202m-fitc-100ul

Applications: FCM, IF(IHC-P), IF(ICC)

Reactivity: Species independent

Conjugate: FITC

Catalog #1: bsm-33202m-hrp-100ul

Applications: IHC-P, IHC-F

Reactivity: Species independent

Conjugate: HRP

Catalog #1: bsm-33202m-pe-100ul

Applications: FCM, IF(IHC-P), IF(ICC)

Reactivity: Species independent

Conjugate: PE

Catalog #1: bsm-33202m-pe-cy5-100ul

Applications: FCM

Reactivity: Species independent

Conjugate: PE-Cy5

Catalog #1: bsm-33202m-pe-Cy5.5-100ul

Applications: FCM

Reactivity: Species independent

Conjugate: PE-Cy5.5

Catalog #1: bsm-33202m-pe-cy7-100ul

Applications: FCM

Reactivity: Species independent

Conjugate: PE-Cy7

Catalog #1: bsm-33202m-percp-100ul

Applications: FCM, IF(IHC-P), IF(ICC)

Reactivity: Species independent

Conjugate: PerCP

Catalog #1: bsm-33202m-percp-Cy5.5-100ul

Applications: FCM, IF(IHC-P), IF(ICC)

Reactivity: Species independent

Conjugate: PerCP-Cy5.5

Catalog #1: bsm-33202m-percp-cy7-100ul

Applications: FCM, IF(IHC-P), IF(ICC)

Reactivity: Species independent

Conjugate: PerCP-Cy7

Catalog #1: bsm-33202m-rbitc-100ul

Applications: FCM, IF(IHC-P), IF(ICC)

Reactivity: Species independent

Conjugate: RBITC

Blocking buffer: 5% NFDM\/TBST\r\nPrimary ab dilution: 1:2000\r\nPrimary ab incubation condition: 2 hours at room temperature\r\nSecondary ab: Goat Anti-Mouse IgG H&L (HRP)\r\nLysate: (-) HeLa, (+) HeLa+Sodium butyrate (30mM, 4hr)\r\nProtein loading quantity: 20 \u03bcg\r\nExposure time: 60 s\r\nPredicted MW: 17 kDa\r\nObserved MW: 17 kDa
Tissue: Human neuroblastoma\r\nSection type: Formalin fixed & Paraffin -embedded section\r\nRetrieval method: High temperature and high pressure\r\nRetrieval buffer: Tris\/EDTA buffer, pH 9.0 Primary ab dilution: 1:200\r\nPrimary ab incubation condition: 1 hour at room temperature\r\nSecondary ab: SP Kit(Mouse)(sp-0024)\r\nCounter stain: Hematoxylin (Blue)\r\nComment: Color brown is the positive signal for bsm-60049M\r\n\r\n\r\n

Catalog #1: bsm-60049m

Applications: WB, IHC-P, IHC-F, IF, ICC/IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Blocking buffer: 5% NFDM\/TBST\r\nPrimary ab dilution: 1:2000\r\nPrimary ab incubation condition: 2 hours at room temperature\r\nSecondary ab: Goat Anti-Mouse IgG H&L (HRP)\r\nLysate: (-) HeLa, (+) HeLa+Sodium butyrate (30mM, 4hr)\r\nProtein loading quantity: 20 \u03bcg\r\nExposure time: 60 s\r\nPredicted MW: 17 kDa\r\nObserved MW: 17 kDa
Tissue: Human neuroblastoma\r\nSection type: Formalin fixed & Paraffin -embedded section\r\nRetrieval method: High temperature and high pressure\r\nRetrieval buffer: Tris\/EDTA buffer, pH 9.0 Primary ab dilution: 1:200\r\nPrimary ab incubation condition: 1 hour at room temperature\r\nSecondary ab: SP Kit(Mouse)(sp-0024)\r\nCounter stain: Hematoxylin (Blue)\r\nComment: Color brown is the positive signal for bsm-60050M\r\n\r\n

Catalog #1: bsm-60050m

Applications: WB, IHC-P, IHC-F, IF, ICC/IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Blocking buffer: 5% NFDM\/TBST\r\nPrimary ab dilution: 1:2000\r\nPrimary ab incubation condition: 2 hours at room temperature\r\nSecondary ab: Goat Anti-Mouse IgG H&L (HRP)\r\nLysate: (-) HeLa, (+) HeLa+Sodium butyrate (30mM, 4hr)\r\nProtein loading quantity: 20 \u03bcg\r\nExposure time: 60 s\r\nPredicted MW: 17 kDa\r\nObserved MW: 17 kDa
Tissue: Human neuroblastoma\r\nSection type: Formalin fixed & Paraffin -embedded section\r\nRetrieval method: High temperature and high pressure\r\nRetrieval buffer: Tris\/EDTA buffer, pH 9.0 Primary ab dilution: 1:200\r\nPrimary ab incubation condition: 1 hour at room temperature\r\nSecondary ab: SP Kit(Mouse)(sp-0024)\r\nCounter stain: Hematoxylin (Blue)\r\nComment: Color brown is the positive signal for bsm-60051M

Catalog #1: bsm-60051m

Applications: WB, IHC-P, IHC-F, IF, ICC/IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated