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Catalog #1: bsm-33197m-bf647-100ul

Applications: WB, FCM, IF(IHC-P), IF(ICC)

Reactivity: Human, Species independent

Conjugate: AbBy Fluor-647

Catalog #1: bsm-33197m-bf680-100ul

Applications: WB, FCM, IF(IHC-P), IF(ICC)

Reactivity: Human, Species independent

Conjugate: AbBy Fluor-680

Catalog #1: bsm-33197m-bf750-100ul

Applications: WB, FCM, IF(IHC-P), IF(ICC)

Reactivity: Human, Species independent

Conjugate: AbBy Fluor-750

Catalog #1: bsm-33197m-apc-100ul

Applications: WB, FCM, IF(IHC-P), IF(ICC)

Reactivity: Human, Species independent

Conjugate: APC

Catalog #1: bsm-33197m-apc-Cy5.5-100ul

Applications: WB, FCM, IF(IHC-P), IF(ICC)

Reactivity: Human, Species independent

Conjugate: APC-Cy5.5

Catalog #1: bsm-33197m-apc-cy7-100ul

Applications: WB, FCM, IF(IHC-P), IF(ICC)

Reactivity: Human, Species independent

Conjugate: APC-Cy7

Catalog #1: bsm-33197m-biotin-100ul

Applications: WB, IHC-P, IHC-F

Reactivity: Human, Species independent

Conjugate: Biotin

Catalog #1: bsm-33197m-cy3-100ul

Applications: WB, FCM, IF(IHC-P), IF(ICC)

Reactivity: Human, Species independent

Conjugate: Cy3

Catalog #1: bsm-33197m-cy5-100ul

Applications: WB, FCM, IF(IHC-P), IF(ICC)

Reactivity: Human, Species independent

Conjugate: Cy5

Catalog #1: bsm-33197m-Cy5.5-100ul

Applications: WB, FCM, IF(IHC-P), IF(ICC)

Reactivity: Human, Species independent

Conjugate: Cy5.5

Catalog #1: bsm-33197m-cy7-100ul

Applications: WB, FCM, IF(IHC-P), IF(ICC)

Reactivity: Human, Species independent

Conjugate: Cy7

Catalog #1: bsm-33197m-fitc-100ul

Applications: WB, FCM, IF(IHC-P), IF(ICC)

Reactivity: Human, Species independent

Conjugate: FITC

Catalog #1: bsm-33197m-hrp-100ul

Applications: WB, IHC-P, IHC-F

Reactivity: Human, Species independent

Conjugate: HRP

Catalog #1: bsm-33197m-pe-100ul

Applications: WB, FCM, IF(IHC-P), IF(ICC)

Reactivity: Human, Species independent

Conjugate: PE

Catalog #1: bsm-33197m-pe-cy5-100ul

Applications: WB, FCM

Reactivity: Human, Species independent

Conjugate: PE-Cy5

Catalog #1: bsm-33197m-pe-Cy5.5-100ul

Applications: WB, FCM

Reactivity: Human, Species independent

Conjugate: PE-Cy5.5

Catalog #1: bsm-33197m-pe-cy7-100ul

Applications: WB, FCM

Reactivity: Human, Species independent

Conjugate: PE-Cy7

Catalog #1: bsm-33197m-percp-100ul

Applications: WB, FCM, IF(IHC-P), IF(ICC)

Reactivity: Human, Species independent

Conjugate: PerCP

Catalog #1: bsm-33197m-percp-Cy5.5-100ul

Applications: WB, FCM, IF(IHC-P), IF(ICC)

Reactivity: Human, Species independent

Conjugate: PerCP-Cy5.5

Catalog #1: bsm-33197m-percp-cy7-100ul

Applications: WB, FCM, IF(IHC-P), IF(ICC)

Reactivity: Human, Species independent

Conjugate: PerCP-Cy7

Catalog #1: bsm-33197m-rbitc-100ul

Applications: WB, FCM, IF(IHC-P), IF(ICC)

Reactivity: Human, Species independent

Conjugate: RBITC

Catalog #1: bsm-34258m-100ul

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P)

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-72357m

Applications: ELISA

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-72360m

Applications: ELISA

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-72358m

Applications: ELISA

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-72362m

Applications: ELISA

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-72363m

Applications: ELISA

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-72364m

Applications: ELISA

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-72365m

Applications: ELISA

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-72366m

Applications: ELISA

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-70215M

Applications: WB, IHC

Reactivity: Human, Mouse, Rat, Bovine

Conjugate: Unconjugated

Catalog #1: bsm-60264m

Applications: WB, IHC-P, IHC-F, IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-72368m

Applications: ELISA

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-51743m

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-70592M

Applications: WB, IP

Reactivity: Human, Mouse, Rat, Chicken

Conjugate: Unconjugated

Catalog #1: bsm-51356M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Blocking buffer: 5% NFDM\/TBST\r\nPrimary Ab dilution: 1:1000\r\nPrimary Ab incubation condition: room temperature 2h\r\nSecondary Ab: Goat Anti-Mouse IgG H&L (HRP)\r\nLysate: 1: Human liver, 2: HepG2, 3: Rat liver, 4: Mouse heart\r\nProtein loading quantity: 20 \u03bcg\r\nExposure time: 30 s\r\nPredicted MW: 58 kDa\r\nObserved MW: 55 kDa\r\n
Tissue: Human heart\r\nSection type: Formalin fixed & Paraffin -embedded section\r\nRetrieval method: High temperature and high pressure\r\nRetrieval buffer: Tris\/EDTA buffer, pH 9.0 Primary Ab dilution: 1:100\r\nPrimary Ab incubation condition: 1 hour at room temperature\r\nSecondary Ab: SP Kit(Mouse)(sp-0024)\r\nCounter stain: Hematoxylin (Blue)\r\nComment: Color brown is the positive signal for bsm-60566M

Catalog #1: bsm-60566m

Applications: WB, IHC-P, IHC-F, IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-60549m

Applications: WB, IHC-P, IHC-F, IF

Reactivity: Human, Rat

Conjugate: Unconjugated

Catalog #1: bsm-60496m

Applications: WB, ICC/IF

Reactivity: Human

Conjugate: Unconjugated

Jurkat cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20\u2103, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with PCNA (3G1) Monoclonal Antibody(bs-R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Paraformaldehyde-fixed, paraffin embedded Rat Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PCNA(Nuclear Loading Control) Monoclonal Antibody, Unconjugated(bsm-33035M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the SP Kit (Mouse, sp-0024) and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PCNA(Nuclear Loading Control) Monoclonal Antibody, Unconjugated(bsm-33035M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the SP Kit (Mouse, sp-0024) and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PCNA(Nuclear Loading Control) Monoclonal Antibody, Unconjugated(bsm-33035M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the SP Kit (Mouse, sp-0024) and DAB (C-0010) staining.

Catalog #1: bsm-33035m

Applications: WB, IHC-P, IHC-F, IF, Flow-Cyt

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

25 ug total protein per lane of various lysates (see on figure) probed with PCNA monoclonal antibody, unconjugated (bsm-10889M) at 1:2000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Rat Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PCNA Monoclonal Antibody, Unconjugated (bsm-10889M) at 1:200 overnight at 4\u00b0C. Followed by conjugated Goat Anti-Mouse IgG antibody (green, bs-0296G-BF488), DAPI (blue, C02-04002) was used to stain the cell nuclei.
Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PCNA Monoclonal Antibody, Unconjugated (bsm-10889M) at 1:200 overnight at 4\u00b0C. Followed by conjugated Goat Anti-Mouse IgG antibody (green, bs-0296G-BF488), DAPI (blue, C02-04002) was used to stain the cell nuclei.

Catalog #1: bsm-10889m-100ul

Applications: WB, IHC-P, IHC-F, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-33035m-bf350-100ul

Applications: WB, IF, Flow-Cyt

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-350

Catalog #1: bsm-10889m-bf350-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-350

Catalog #1: bsm-33035m-bf405-100ul

Applications: WB, IF, Flow-Cyt

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-405

Catalog #1: bsm-10889m-bf405-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-405

Catalog #1: bsm-33035m-bf488-100ul

Applications: WB, IF, Flow-Cyt

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-488

Catalog #1: bsm-10889m-bf488-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-488

Catalog #1: bsm-33035m-bf555-100ul

Applications: WB, IF, Flow-Cyt

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-555