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Catalog #1: bsm-43072M-PE-Cy7

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: PE-Cy7

Catalog #1: bsm-43072m-percp-100ul

Applications: IF(IHC-P)

Reactivity: Human

Conjugate: PerCP

Catalog #1: bsm-43072m-percp-Cy5.5-100ul

Applications: IF(IHC-P)

Reactivity: Human

Conjugate: PerCP-Cy5.5

Catalog #1: bsm-43072m-percp-cy7-100ul

Applications: IF(IHC-P)

Reactivity: Human

Conjugate: PerCP-Cy7

Catalog #1: bsm-43072m-rbitc-100ul

Applications: IF(IHC-P)

Reactivity: Human

Conjugate: RBITC

Catalog #1: bsm-43073M

Applications: WB, FCM

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-43073M-BF350

Applications: WB, FCM

Reactivity: Human

Conjugate: AbBy Fluor-350

Catalog #1: bsm-43073M-BF405

Applications: WB, FCM

Reactivity: Human

Conjugate: AbBy Fluor-405

Catalog #1: bsm-43073M-BF488

Applications: WB, FCM

Reactivity: Human

Conjugate: AbBy Fluor-488

Catalog #1: bsm-43073M-BF555

Applications: WB, FCM

Reactivity: Human

Conjugate: AbBy Fluor-555

Catalog #1: bsm-43073M-BF594

Applications: WB, FCM

Reactivity: Human

Conjugate: AbBy Fluor-594

Catalog #1: bsm-43073M-BF647

Applications: WB, FCM

Reactivity: Human

Conjugate: AbBy Fluor-647

Catalog #1: bsm-43073M-BF680

Applications: WB, FCM

Reactivity: Human

Conjugate: AbBy Fluor-680

Catalog #1: bsm-43073M-BF750

Applications: WB, FCM

Reactivity: Human

Conjugate: AbBy Fluor-750

Catalog #1: bsm-43073M-APC

Applications: WB, FCM

Reactivity: Human

Conjugate: APC

Catalog #1: bsm-43073M-APC-Cy5.5

Applications: WB, FCM

Reactivity: Human

Conjugate: APC-Cy5.5

Catalog #1: bsm-43073M-APC-Cy7

Applications: WB, FCM

Reactivity: Human

Conjugate: APC-Cy7

Catalog #1: bsm-43073M-Biotin

Applications: WB

Reactivity: Human

Conjugate: Biotin

Catalog #1: bsm-43073M-Cy3

Applications: WB, FCM

Reactivity: Human

Conjugate: Cy3

Catalog #1: bsm-43073M-Cy5

Applications: WB, FCM

Reactivity: Human

Conjugate: Cy5

Catalog #1: bsm-43073M-Cy5.5

Applications: WB, FCM

Reactivity: Human

Conjugate: Cy5.5

Catalog #1: bsm-43073M-Cy7

Applications: WB, FCM

Reactivity: Human

Conjugate: Cy7

Catalog #1: bsm-43073M-FITC

Applications: WB, FCM

Reactivity: Human

Conjugate: FITC

Catalog #1: bsm-43073M-HRP

Applications: WB

Reactivity: Human

Conjugate: HRP

Catalog #1: bsm-43073M-PE

Applications: WB, FCM

Reactivity: Human

Conjugate: PE

Catalog #1: bsm-43073M-PE-Cy5

Applications: WB, FCM

Reactivity: Human

Conjugate: PE-Cy5

Catalog #1: bsm-43073M-PE-Cy5.5

Applications: WB, FCM

Reactivity: Human

Conjugate: PE-Cy5.5

Catalog #1: bsm-43073M-PE-Cy7

Applications: WB, FCM

Reactivity: Human

Conjugate: PE-Cy7

Catalog #1: bsm-43073M-PerCP

Applications: WB, FCM

Reactivity: Human

Conjugate: PerCP

Catalog #1: bsm-43073M-PerCP-Cy5.5

Applications: WB, FCM

Reactivity: Human

Conjugate: PerCP-Cy5.5

Catalog #1: bsm-43073M-RBITC

Applications: WB, FCM

Reactivity: Human

Conjugate: RBITC

Catalog #1: bsm-70603M

Applications: WB, IP

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-70604M

Applications: WB, IP

Reactivity: Human

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded (human placenta); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (PD-L1) Monoclonal Antibody, Unconjugated (bsm-54472R) at 1200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human lung carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (PD-L1) Monoclonal Antibody, Unconjugated (bsm-54472R) at 1200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human cervical carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (PD-L1) Monoclonal Antibody, Unconjugated (bsm-54472R) at 1200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
25 ug total protein per lane of various lysates (see on figure) probed with PD-L1 monoclonal antibody, unconjugated (bsm-54472R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Catalog #1: bsm-54472r

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51708m

Applications: WB, IHC-P, IHC-F, IF

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-51098M

Applications: WB

Reactivity: Mouse

Conjugate: Unconjugated

Catalog #1: bsm-60337m

Applications: WB, ICC/IF

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51338M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-60401m

Applications: WB, ICC/IF

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-70600M

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Human Lung Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PDK2 Monoclonal Antibody, Unconjugated (bsm-51043M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PDK2 Monoclonal Antibody, Unconjugated (bsm-51043M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PDK2 Monoclonal Antibody, Unconjugated (bsm-51043M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
25 ug total protein per lane of various lysates (see on figure) probed with PDK2 monoclonal antibody, unconjugated (bsm-51043M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Catalog #1: bsm-51043m

Applications: WB, IHC-P, IHC-F, IF

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bsm-51108M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51665m

Applications: WB, IHC-P, IHC-F, IF

Reactivity:

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded (human lymphoid); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (CD31) Monoclonal Antibody, Unconjugated (bsm-56229M) at 1:100 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human Placenta); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (CD31) Monoclonal Antibody, Unconjugated (bsm-56229M) at 1:100 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.

Catalog #1: bsm-56229m

Applications: IHC

Reactivity: Human

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded (human lung carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (CD31) Monoclonal Antibody, Unconjugated (bsm-56214M) at 1:150 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (CD31) Monoclonal Antibody, Unconjugated (bsm-56214M) at 1:150 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human liver carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (CD31) Monoclonal Antibody, Unconjugated (bsm-56214M) at 1:150 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human liver ); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (CD31) Monoclonal Antibody, Unconjugated (bsm-56214M) at 1:150 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.

Catalog #1: bsm-56214m

Applications: IHC-P, IHC-F, IF

Reactivity: Rat

Conjugate: Unconjugated

Catalog #1: bsm-70221M

Applications: WB, IHC

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Lane 1: Mouse Spleen tissue lysates; Lane 2: Mouse Liver tissue lysates; Lane 3: Mouse Cerebrum tissue lysates; Lane 4: Rat Spleen tissue lysates ; Lane 5: Rat Cerebrum tissue lysates; Lane 6: Human Jurkat cell lysates; Lane 7: Human Raji cell lysates; Lane 8: Human MCF-7 cell lysates; Lane 9: Human A431 cell lysates; Lane 10: Human Hela cell lysates probed with Peroxiredoxin 1 Monoclonal Antibody, Unconjugated (bsm-33118M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
MCF7 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (Peroxiredoxin 1) polyclonal Antibody, Unconjugated (bsm-33118M) 1:25, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Mouse IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
Hela cells were\u00a0fixed with 4% PFA for 10min at room temperature,permeabilized with\u00a090% ice-cold methanol for 20 min at -20\u2103,\u00a0and incubated in 5% BSA blocking buffer\u00a0for 30 min at room temperature. Cells were then stained with Peroxiredoxin 1 Antibody(bsm-33118M)at 1:200 dilution\u00a0in blocking buffer and\u00a0incubated for 30 min at\u00a0room temperature,\u00a0washed twice with 2%BSA in PBS,\u00a0followed by\u00a0secondary antibody incubation\u00a0for 40 min\u00a0at\u00a0room temperature. Acquisitions of 20,000 events were performed.\u00a0Cells stained with primary antibody\u00a0(green), and isotype control (orange).

Catalog #1: bsm-33118m-100ul

Applications: WB, FCM, IHC-P, IHC-F, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-33118m-bf350-100ul

Applications: WB, FCM, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-350