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Catalog #1: bsm-52219m-hrp-100ul

Applications: WB, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: HRP

Catalog #1: bsm-52219m-pe-100ul

Applications: WB, IF

Reactivity: Human, Mouse, Rat

Conjugate: PE

Catalog #1: bsm-52219m-pe-cy5-100ul

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: PE-Cy5

Catalog #1: bsm-52219m-pe-Cy5.5-100ul

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: PE-Cy5.5

Catalog #1: bsm-52219m-pe-cy7-100ul

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: PE-Cy7

Catalog #1: bsm-52219m-percp-100ul

Applications: WB, IF

Reactivity: Human, Mouse, Rat

Conjugate: PerCP

Catalog #1: bsm-52219m-percp-Cy5.5-100ul

Applications: WB, IF

Reactivity: Human, Mouse, Rat

Conjugate: PerCP-Cy5.5

Catalog #1: bsm-52219m-percp-cy7-100ul

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: PerCP-Cy7

Catalog #1: bsm-52219m-rbitc-100ul

Applications: WB, IF

Reactivity: Human, Mouse, Rat

Conjugate: RBITC

BRL-3A cell lysates probed with PI3K p85 (5C11) Monoclonal Antibody, unconjugated (bsm-33219M) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Lane 1: NIH\/3T3 Cell lysates; Lane 2: RAW246.7 Cell lysates; probed with PI3K p85 (5C11) Monoclonal Antibody, unconjugated (bsm-33219M) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Lane 1: Human Jurkatcell lysates; Lane 2: Mouse NIH\/3T3 cell lysates; Lane 3: Mouse Cerebrum lysates; Lane 4: Rat Cerebrum lysates; Lane 5: Mouse Heart lysates; Lane 6: Rat Heart lysates; Lane 7: Mouse Skeletal muscle lysates; Lane 8: Rat Skeletal muscle lysates; Lane 9: Mouse Spleen lysates; Lane 10: Human MOLT-4 cell lysates probed with PI3K p85 Monoclonal Antibody, Unconjugated (bsm-33219M ) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bsm-33219M

Applications: WB, IHC-P, IHC-F, IF, ICC/IF

Reactivity: Human, Mouse, Rat, Chicken

Conjugate: Unconjugated

Catalog #1: bsm-33219M-BF350

Applications: WB, IF

Reactivity: Human, Mouse, Rat, Chicken

Conjugate: AbBy Fluor-350

Catalog #1: bsm-33219M-BF405

Applications: WB, IF

Reactivity: Human, Mouse, Rat, Chicken

Conjugate: AbBy Fluor-405

Catalog #1: bsm-33219M-BF488

Applications: WB, IF

Reactivity: Human, Mouse, Rat, Chicken

Conjugate: AbBy Fluor-488

Catalog #1: bsm-33219M-BF555

Applications: WB, IF

Reactivity: Human, Mouse, Rat, Chicken

Conjugate: AbBy Fluor-555

Catalog #1: bsm-33219M-BF594

Applications: WB, IF

Reactivity: Human, Mouse, Rat, Chicken

Conjugate: AbBy Fluor-594

Catalog #1: bsm-33219M-BF647

Applications: WB, IF

Reactivity: Human, Mouse, Rat, Chicken

Conjugate: AbBy Fluor-647

Catalog #1: bsm-33219M-BF680

Applications: WB, IF

Reactivity: Human, Mouse, Rat, Chicken

Conjugate: AbBy Fluor-680

Catalog #1: bsm-33219M-BF750

Applications: WB, IF

Reactivity: Human, Mouse, Rat, Chicken

Conjugate: AbBy Fluor-750

Catalog #1: bsm-33219M-APC

Applications: WB, IF

Reactivity: Human, Mouse, Rat, Chicken

Conjugate: APC

Catalog #1: bsm-33219M-APC-Cy5.5

Applications: WB, IF

Reactivity: Human, Mouse, Rat, Chicken

Conjugate: APC-Cy5.5

Catalog #1: bsm-33219M-APC-Cy7

Applications: WB

Reactivity: Human, Mouse, Rat, Chicken

Conjugate: APC-Cy7

Catalog #1: bsm-33219M-Biotin

Applications: WB, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Chicken

Conjugate: Biotin

Catalog #1: bsm-33219M-Cy3

Applications: WB, IF

Reactivity: Human, Mouse, Rat, Chicken

Conjugate: Cy3

Catalog #1: bsm-33219M-Cy5

Applications: WB, IF

Reactivity: Human, Mouse, Rat, Chicken

Conjugate: Cy5

Catalog #1: bsm-33219M-Cy5.5

Applications: WB, IF

Reactivity: Human, Mouse, Rat, Chicken

Conjugate: Cy5.5

Catalog #1: bsm-33219M-Cy7

Applications: WB, IF

Reactivity: Human, Mouse, Rat, Chicken

Conjugate: Cy7

Catalog #1: bsm-33219M-FITC

Applications: WB, IF

Reactivity: Human, Mouse, Rat, Chicken

Conjugate: FITC

Catalog #1: bsm-33219M-HRP

Applications: WB, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Chicken

Conjugate: HRP

Catalog #1: bsm-33219M-PE

Applications: WB, IF

Reactivity: Human, Mouse, Rat, Chicken

Conjugate: PE

Catalog #1: bsm-33219M-PE-Cy5

Applications: WB

Reactivity: Human, Mouse, Rat, Chicken

Conjugate: PE-Cy5

Catalog #1: bsm-33219M-PE-Cy5.5

Applications: WB

Reactivity: Human, Mouse, Rat, Chicken

Conjugate: PE-Cy5.5

Catalog #1: bsm-33219M-PE-Cy7

Applications: WB

Reactivity: Human, Mouse, Rat, Chicken

Conjugate: PE-Cy7

Catalog #1: bsm-33219M-PerCP

Applications: WB, IF

Reactivity: Human, Mouse, Rat, Chicken

Conjugate: PerCP

Catalog #1: bsm-33219M-PerCP-Cy5.5

Applications: WB, IF

Reactivity: Human, Mouse, Rat, Chicken

Conjugate: PerCP-Cy5.5

Catalog #1: bsm-33219M-RBITC

Applications: WB, IF

Reactivity: Human, Mouse, Rat, Chicken

Conjugate: RBITC

Blocking buffer: 5% NFDM\/TBST\r\nPrimary Ab dilution: 1:1000\r\nPrimary Ab incubation condition:4\u00b0C overnight\r\nLysate: 1: LnCap, 2: K562, 3: NIH\/3T3, 4: BRL\r\nProtein loading quantity: 20 \u03bcg\r\nExposure time: 60 s\r\nPredicted MW: 85 kDa\r\nObserved MW: 85 kDa
Tissue: Mouse colon\r\nSection type: Formalin fixed & Paraffin -embedded section\r\nRetrieval method: High temperature and high pressure\r\nRetrieval buffer: Tris\/EDTA buffer, pH 9.0 Primary Ab dilution: 1:100\r\nPrimary Ab incubation condition: 1 hour at room temperature\r\nCounter stain: Hematoxylin\r\nComment: Color brown is the positive signal for bsm-60360M
Tissue: human colon\r\nSection type: Formalin fixed & Paraffin -embedded section\r\nRetrieval method: High temperature and high pressure\r\nRetrieval buffer: Tris\/EDTA buffer, pH 9.0 Primary Ab dilution: 1:100\r\nPrimary Ab incubation condition: 1 hour at room temperature\r\nCounter stain: Hematoxylin\r\nComment: Color brown is the positive signal for bsm-60360M

Catalog #1: bsm-60360m

Applications: WB, IHC-P, IHC-F, IF

Reactivity: Human, Mouse

Conjugate: Unconjugated

25 ug total protein per lane of various lysates (see on figure) probed with PIN1 monoclonal antibody, unconjugated (bsm-51230M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PIN1 Monoclonal Antibody, Unconjugated (bsm-51230M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PIN1 Monoclonal Antibody, Unconjugated (bsm-51230M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PIN1 Monoclonal Antibody, Unconjugated (bsm-51230M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.

Catalog #1: bsm-51230m-100ul

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-51514m

Applications: WB

Reactivity: Mouse

Conjugate: Unconjugated

Lane 1: 293T; Lane 2: A431 probed with bsm-51265M PINK1 (38CT20.8.5) Monoclonal Antibody at 1:2000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Paraformaldehyde-fixed, paraffin embedded Human stomach tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 15 minutes; Blocking buffer (3% BSA) at room temperature for 30min; Antibody incubation with PINK1 (38CT20.8.5) Monoclonal Antibody (bsm-51265M) at 1:25 for 1 hour at 37\u00b0C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human heart tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 15 minutes; Blocking buffer (3% BSA) at room temperature for 30min; Antibody incubation with PINK1 (38CT20.8.5) Monoclonal Antibody (bsm-51265M) at 1:25 for 1 hour at 37\u00b0C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.

Catalog #1: bsm-51265m

Applications: WB, IHC-P, IHC-F, IF, ICC/IF

Reactivity: Human

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat colitis tissue abeled with Anti-M2-PK Polyclonal Antibody, Unconjugated(bs-0102M) 1:200 followed by conjugation to the secondary antibody.
Molt-4 lysates probed with PKM2 Polyclonal Antibody, Unconjugated (bs-0102M) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
Lane 1: Human A549 cell lysates; Lane 2: Human U-87MG cell lysates; Lane 3: Human U251 cell lysates probed with M2-PK Polyclonal Antibody, Unconjugated (bs-0102M) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (PKM2) polyclonal Antibody, Unconjugated (bs-0102M) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Mouse IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

Catalog #1: bs-0102M

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-41330m-100ul

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-41330m-bf350-100ul

Applications: WB

Reactivity: Human

Conjugate: AbBy Fluor-350

Catalog #1: bsm-41330m-bf405-100ul

Applications: WB

Reactivity: Human

Conjugate: AbBy Fluor-405

Catalog #1: bsm-41330m-bf488-100ul

Applications: WB

Reactivity: Human

Conjugate: AbBy Fluor-488

Catalog #1: bsm-41330m-bf555-100ul

Applications: WB

Reactivity: Human

Conjugate: AbBy Fluor-555

Catalog #1: bsm-41330m-bf594-100ul

Applications: WB

Reactivity: Human

Conjugate: AbBy Fluor-594

Catalog #1: bsm-41330m-bf647-100ul

Applications: WB

Reactivity: Human

Conjugate: AbBy Fluor-647

Catalog #1: bsm-41330m-bf680-100ul

Applications: WB

Reactivity: Human

Conjugate: AbBy Fluor-680