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Catalog #1: bsm-72390m

Applications: ELISA

Reactivity:

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded (human lung carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (RPS6 (9F2)) Monoclonal Antibody, Unconjugated (bsm-51406M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.\r\n\r\n
Paraformaldehyde-fixed, paraffin embedded (human cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (RPS6 (9F2)) Monoclonal Antibody, Unconjugated (bsm-51406M) at 1:100 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.\r\n\r\n
25 ug total protein per lane of various lysates (see on figure) probed with RPS6 (N-term) monoclonal antibody, unconjugated (bsm-51406M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with RPS6 (N-term) Monoclonal Antibody, Unconjugated (bsm-51406M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.

Catalog #1: bsm-51406m

Applications: WB, IHC-P, IHC-F, IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-51591m-100ul

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51052M

Applications: WB

Reactivity: Human, Mouse

Conjugate: Unconjugated

Lane 1 : HeLa Cell Lysate; Lane 2 : A431 Cell Lysate; Lane 3 : C6 Cell Lysate; probed with bsm-50240M p90RSK1 (8C12) Monoclonal Antibody at 2.0ug\/mL (1:500) and incubated at 4\u2103 overnight, followed by secondary antibody incubation for 60min at room temperature.
Immunoprecipitation of p90RSK1 from A431 cell line using p90RSK1 (8C12) Monoclonal Antibody (bsm-50240M). Lane 1 : A431 Cell Lysate; Lane 2 : Precipitated from 200ug of A431 Cell Lysate using 2ug of antibody; Lane 3 : Precipitated from 200ug of A431 Cell Lysate using 5ug of antibody; Lane 4 : Precipitated from PBS using 5ug of antibody. WB analysis was performed using bsm-50240M at 1.0ug\/mL (1:1000) and incubated at 4\u2103 overnight, followed by secondary antibody incubation for 60min at room temperature.

Catalog #1: bsm-50240M

Applications: WB, ELISA

Reactivity:

Conjugate: Unconjugated

Lane 1: K562 cell lysates probed with RPS6KA3 Monoclonal Antibody, Unconjugated (bsm-51509M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Mouse Heart tissue lysates probed with RPS6KA3 Monoclonal Antibody, Unconjugated (bsm-51509M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bsm-51509m

Applications: WB

Reactivity: Human, Mouse

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with RPS6KB1 Monoclonal Antibody, Unconjugated (bsm-33264M) at 1:400 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with RPS6KB1 Monoclonal Antibody, Unconjugated (bsm-33264M) at 1:400 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human brain glioma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with RPS6KB1 Monoclonal Antibody, Unconjugated (bsm-33264M) at 1:400 overnight at 4\u00b0C, DAB staining.

Catalog #1: bsm-33264M

Applications: IHC-P, IHC-F, IF(ICC), IF

Reactivity: Human, Rat

Conjugate: Unconjugated

Catalog #1: bsm-33264M-BF350

Applications: IF(ICC), IF

Reactivity: Human, Rat

Conjugate: AbBy Fluor-350

Catalog #1: bsm-33264M-BF405

Applications: IF(ICC), IF

Reactivity: Human, Rat

Conjugate: AbBy Fluor-405

Catalog #1: bsm-33264M-BF488

Applications: IF(ICC), IF

Reactivity: Human, Rat

Conjugate: AbBy Fluor-488

Catalog #1: bsm-33264M-BF555

Applications: IF(ICC), IF

Reactivity: Human, Rat

Conjugate: AbBy Fluor-555

Catalog #1: bsm-33264M-BF594

Applications: IF(ICC), IF

Reactivity: Human, Rat

Conjugate: AbBy Fluor-594

Catalog #1: bsm-33264M-BF647

Applications: IF(ICC), IF

Reactivity: Human, Rat

Conjugate: AbBy Fluor-647

Catalog #1: bsm-33264M-BF680

Applications: IF(ICC), IF

Reactivity: Human, Rat

Conjugate: AbBy Fluor-680

Catalog #1: bsm-33264M-BF750

Applications: IF(ICC), IF

Reactivity: Human, Rat

Conjugate: AbBy Fluor-750

Catalog #1: bsm-33264M-APC

Applications: IF(ICC), IF

Reactivity: Human, Rat

Conjugate: APC

Catalog #1: bsm-33264M-APC-Cy5.5

Applications: IF(ICC), IF

Reactivity: Human, Rat

Conjugate: APC-Cy5.5

Catalog #1: bsm-33264M-APC-Cy7

Applications: IF(ICC)

Reactivity: Human, Rat

Conjugate: APC-Cy7

Catalog #1: bsm-33264M-Biotin

Applications: IHC-P, IHC-F

Reactivity: Human, Rat

Conjugate: Biotin

Catalog #1: bsm-33264M-Cy3

Applications: IF(ICC), IF

Reactivity: Human, Rat

Conjugate: Cy3

Catalog #1: bsm-33264M-Cy5

Applications: IF(ICC), IF

Reactivity: Human, Rat

Conjugate: Cy5

Catalog #1: bsm-33264M-Cy5.5

Applications: IF(ICC), IF

Reactivity: Human, Rat

Conjugate: Cy5.5

Catalog #1: bsm-33264M-Cy7

Applications: IF(ICC), IF

Reactivity: Human, Rat

Conjugate: Cy7

Catalog #1: bsm-33264M-FITC

Applications: IF(ICC), IF

Reactivity: Human, Rat

Conjugate: FITC

Catalog #1: bsm-33264M-HRP

Applications: IHC-P, IHC-F

Reactivity: Human, Rat

Conjugate: HRP

Catalog #1: bsm-33264M-PerCP

Applications: IF(ICC), IF

Reactivity: Human, Rat

Conjugate: PerCP

Catalog #1: bsm-33264M-PerCP-Cy5.5

Applications: IF(ICC), IF

Reactivity: Human, Rat

Conjugate: PerCP-Cy5.5

Catalog #1: bsm-33264M-RBITC

Applications: IF(ICC), IF

Reactivity: Human, Rat

Conjugate: RBITC

Lane 1: Sample Tissue\/Cell lysates; Lane 2: Sample Tissue\/Cell lysates; Lane 3: Sample Tissue\/Cell lysates; probed with antibodyname Monoclonal Antibody, unconjugated (bsm-51388M) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Lane 1: Jurkat Cell lysates; Lane 2: MCF-7 Cell lysates; Lane 3: NIH\/3T3 Cell lysates; probed with RPS6KB2 (164CT21.2.2) Monoclonal Antibody, unconjugated (bsm-51388M) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

Catalog #1: bsm-51388M

Applications: WB

Reactivity: Human, Mouse

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Human liver cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with RPS6KB2 Monoclonal Antibody, Unconjugated (bsm-51475M) at 1:200 overnight at 4\u00b0C, DAB staining.
Lane 1: Jurkat cell lysates probed with RPS6KB2 Monoclonal Antibody, Unconjugated (bsm-51475M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Mouse NIH\/3T3 cell lysates probed with RPS6KB2 Monoclonal Antibody, Unconjugated (bsm-51475M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bsm-51475m

Applications: WB

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-51335M

Applications: WB

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-60351m

Applications: WB, ICC/IF

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-72392m

Applications: WB, ELISA

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-72393m

Applications: WB, ELISA

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-72394m

Applications: WB, ELISA

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-72395m

Applications: WB, ELISA

Reactivity:

Conjugate: Unconjugated

Blocking buffer: 5% NFDM\/TBST\r\nPrimary ab dilution: 1:2000\r\nPrimary ab incubation condition: room temperature 2h\r\nSecondary ab: Goat Anti-Mouse IgG H&L (HRP)\r\nLysate: Arabidopsis thaliana\r\nProtein loading quantity: 5 \u03bcg\r\nExposure time: 10 s\r\nPredicted MW: 55 kDa\r\nObserved MW: 55 kDa
Blocking buffer: 5% NFDM\/TBST\r\nPrimary ab dilution: 1:2000\r\nPrimary ab incubation condition: room temperature 2h\r\nSecondary ab: Goat Anti-Mouse IgG H&L (HRP)\r\nLysate: Rice\r\nProtein loading quantity: 0.008 \u03bcg\r\nExposure time: 10 s\r\nPredicted MW: 55 kDa\r\nObserved MW: 55 kDa

Catalog #1: bsm-60435m

Applications: WB

Reactivity: Arab

Conjugate: Unconjugated

Catalog #1: bsm-33226m-100ul

Applications: WB

Reactivity: Arabidopsis Thaliana

Conjugate: Unconjugated

Catalog #1: bsm-33226m-bf350-100ul

Applications: WB

Reactivity: Arabidopsis Thaliana

Conjugate: AbBy Fluor-350

Catalog #1: bsm-33226m-bf405-100ul

Applications: WB

Reactivity: Arabidopsis Thaliana

Conjugate: AbBy Fluor-405

Catalog #1: bsm-33226m-bf488-100ul

Applications: WB

Reactivity: Arabidopsis Thaliana

Conjugate: AbBy Fluor-488

Catalog #1: bsm-33226m-bf555-100ul

Applications: WB

Reactivity: Arabidopsis Thaliana

Conjugate: AbBy Fluor-555

Catalog #1: bsm-33226m-bf594-100ul

Applications: WB

Reactivity: Arabidopsis Thaliana

Conjugate: AbBy Fluor-594

Catalog #1: bsm-33226m-bf647-100ul

Applications: WB

Reactivity: Arabidopsis Thaliana

Conjugate: AbBy Fluor-647

Catalog #1: bsm-33226m-bf680-100ul

Applications: WB

Reactivity: Arabidopsis Thaliana

Conjugate: AbBy Fluor-680

Catalog #1: bsm-33226m-bf750-100ul

Applications: WB

Reactivity: Arabidopsis Thaliana

Conjugate: AbBy Fluor-750

Catalog #1: bsm-33226m-apc-100ul

Applications: WB

Reactivity: Arabidopsis Thaliana

Conjugate: APC

Catalog #1: bsm-33226m-apc-Cy5.5-100ul

Applications: WB

Reactivity: Arabidopsis Thaliana

Conjugate: APC-Cy5.5