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Catalog #1: bsm-41703m-percp-cy7-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human

Conjugate: PerCP-Cy7

Catalog #1: bsm-41704m-percp-cy7-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human

Conjugate: PerCP-Cy7

Catalog #1: bsm-41706m-percp-cy7-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human

Conjugate: PerCP-Cy7

Catalog #1: bsm-41703m-rbitc-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human

Conjugate: RBITC

Catalog #1: bsm-41704m-rbitc-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human

Conjugate: RBITC

Catalog #1: bsm-41706m-rbitc-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human

Conjugate: RBITC

Catalog #1: bsm-70356M

Applications: WB, IP

Reactivity: Human

Conjugate: Unconjugated

Immunohistochemical analysis of CD276 Antibody in MCF-7 cell sections. Cell was fixed with formaldehyde and blocked with super block for 10 minutes at room temperature; antigen retrieval was by heat mediation with a EDTA buffer (pH9. 0). Samples were incubated with primary antibody (1:25) for 1 hours at room temperature. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
Immunohistochemical analysis of CD276 Antibody in 293T(untransfected) cell sections. Cell was fixed with formaldehyde and blocked with super block for 10 minutes at room temperature; antigen retrieval was by heat mediation with a EDTA buffer (pH9. 0). Samples were incubated with primary antibody (1:500) for 1 hours at room temperature. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
Paraformaldehyde-fixed, paraffin embedded Human tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with CD276 Monoclonal Antibody, Unconjugated (bsm-51711M) at 1:400 overnight at 4\u00b0C, DAB staining.

Catalog #1: bsm-51711m

Applications: WB, IHC-P, IHC-F, IF

Reactivity:

Conjugate: Unconjugated

Blocking buffer: 5% NFDM\/TBST\r\nPrimary ab dilution: 1:1000\r\nPrimary ab incubation condition: 2 hours at room temperature\r\nSecondary ab: Goat Anti-Rabbit IgG H&L (HRP)\r\nLysate: 1: HeLa, 2: SH-SY5Y\r\nProtein loading quantity: 20 \u03bcg\r\nExposure time: 3 s\r\nPredicted MW: 150 kDa\r\nObserved MW: 150 kDa
Blocking buffer: 5% NFDM\/TBST\r\nPrimary ab dilution: 1:1000\r\nPrimary ab incubation condition: 2 hours at room temperature\r\nSecondary ab: Goat Anti-Rabbit IgG H&L (HRP)\r\nLysate: 1: RAW 267.7, 2: A20\r\nProtein loading quantity: 20 \u03bcg\r\nExposure time: 30 s\r\nPredicted MW: 150 kDa\r\nObserved MW: 150 kDa
Tissue: Human breast cancer\r\nSection type: Formalin fixed & Paraffin -embedded section\r\nRetrieval method: High temperature and high pressure\r\nRetrieval buffer: Tris\/EDTA buffer, pH 9.0 Primary ab dilution: 1:100\r\nPrimary ab incubation condition: 1 hour at room temperature\r\nSecondary ab: SP Kit(Mouse)(sp-0024)\r\nCounter stain: Hematoxylin (Blue)\r\nComment: Color brown is the positive signal for bsm-60485M
Tissue: Mouse testis\r\nSection type: Formalin fixed & Paraffin -embedded section\r\nRetrieval method: High temperature and high pressure\r\nRetrieval buffer: Tris\/EDTA buffer, pH 9.0 Primary ab dilution: 1:100\r\nPrimary ab incubation condition: 1 hour at room temperature\r\nSecondary ab: SP Kit(Mouse)(sp-0024)\r\nCounter stain: Hematoxylin (Blue)\r\nComment: Color brown is the positive signal for bsm-60485M

Catalog #1: bsm-60485m

Applications: WB, IHC-P, IHC-F, IF, ICC/IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1: K562 Cell lysates; Lane 2: mouse brain lysates; Lane 3: U-251 MG Cell lysates; probed with BAIAP2 (1037CT15.1.3) Monoclonal Antibody, unconjugated (bsm-51410M) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Lane 1: Cerebrum lysates probed with BAIAP2 (4G7) Monoclonal Antibody, Unconjugated (bsm-51410M) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bsm-51410M

Applications: WB

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bsm-51349M

Applications: WB, IHC-P

Reactivity: Human

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded human brain tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 15 minutes; Blocking buffer (3% BSA) at room temperature for 30min; Antibody incubation with BAP31 (2C6) Monoclonal Antibody (bsm-51442M) at 1:25 for 1 hour at 37\u00b0C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.
Lane 1: A431 Cell lysates; Lane 2: NCCIT Cell lysates; probed with BAP31 (2C6) Monoclonal Antibody, unconjugated (bsm-51442M) at 1:2000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

Catalog #1: bsm-51442M

Applications: WB, IHC-P

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-0910M

Applications: ELISA, IHC-P, IHC-F, IF

Reactivity: Other Species

Conjugate: Unconjugated

Catalog #1: bsm-0910M-BF350

Applications: IF

Reactivity: Other Species

Conjugate: AbBy Fluor-350

Catalog #1: bsm-0910M-BF405

Applications: IF

Reactivity: Other Species

Conjugate: AbBy Fluor-405

Catalog #1: bsm-0910M-BF488

Applications: IF

Reactivity: Other Species

Conjugate: AbBy Fluor-488

Catalog #1: bsm-0910M-BF555

Applications: IF

Reactivity: Other Species

Conjugate: AbBy Fluor-555

Catalog #1: bsm-0910M-BF594

Applications: IF

Reactivity: Other Species

Conjugate: AbBy Fluor-594

Catalog #1: bsm-0910M-BF647

Applications: IF

Reactivity: Other Species

Conjugate: AbBy Fluor-647

Catalog #1: bsm-0910M-BF680

Applications: IF

Reactivity: Other Species

Conjugate: AbBy Fluor-680

Catalog #1: bsm-0910M-BF750

Applications: IF

Reactivity: Other Species

Conjugate: AbBy Fluor-750

Catalog #1: bsm-0910M-APC

Applications: IF

Reactivity: Other Species

Conjugate: APC

Catalog #1: bsm-0910M-APC-Cy5.5

Applications: IF

Reactivity: Other Species

Conjugate: APC-Cy5.5

Catalog #1: bsm-0910M-APC-Cy7

Applications: WB, IF(IHC-P)

Reactivity: Other Species

Conjugate: APC-Cy7

Catalog #1: bsm-0910M-Biotin

Applications: ELISA, IHC-P, IHC-F

Reactivity: Other Species

Conjugate: Biotin

Catalog #1: bsm-0910M-Cy3

Applications: IF

Reactivity: Other Species

Conjugate: Cy3

Catalog #1: bsm-0910M-Cy5

Applications: IF

Reactivity: Other Species

Conjugate: Cy5

Catalog #1: bsm-0910M-Cy5.5

Applications: IF

Reactivity: Other Species

Conjugate: Cy5.5

Catalog #1: bsm-0910M-Cy7

Applications: IF

Reactivity: Other Species

Conjugate: Cy7

Catalog #1: bsm-0910M-FITC

Applications: IF

Reactivity: Other Species

Conjugate: FITC

Catalog #1: bsm-0910M-HRP

Applications: ELISA, IHC-P, IHC-F

Reactivity: Other Species

Conjugate: HRP

Catalog #1: bsm-0910M-PE

Applications: IF

Reactivity: Other Species

Conjugate: PE

Catalog #1: bsm-0910M-PE-Cy5

Applications: WB

Reactivity: Other Species

Conjugate: PE-Cy5

Catalog #1: bsm-0910M-PE-Cy5.5

Applications: WB

Reactivity: Other Species

Conjugate: PE-Cy5.5

Catalog #1: bsm-0910M-PE-Cy7

Applications: WB

Reactivity: Other Species

Conjugate: PE-Cy7

Catalog #1: bsm-0910M-PerCP

Applications: IF

Reactivity: Other Species

Conjugate: PerCP

Catalog #1: bsm-0910M-PerCP-Cy5.5

Applications: IF

Reactivity: Other Species

Conjugate: PerCP-Cy5.5

Catalog #1: bsm-0910M-RBITC

Applications: IF

Reactivity: Other Species

Conjugate: RBITC

Paraformaldehyde-fixed, paraffin embedded Human brain glioma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Bax Monoclonal Antibody, Unconjugated (bsm-33279M) at 1:300 overnight at 4\u00b0C, followed by Goat Anti-Mouse IgG (H+L) Cy3 conjugated secondary antibody. DAPI staining of the nucleus was done and then detected.
Lane 1: Cerebrum lysates probed with Bax Monoclonal Antibody, Unconjugated (bsm-33279M) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Tissue\/cell: Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (Bax) monoclonal Antibody, Unconjugated (bs-33279M) 1:100, 90 minutes at 37\u00b0C; followed by a CY3 conjugated Goat Anti-Mouse IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
Paraformaldehyde-fixed, paraffin embedded (human gastric carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (Bax ) Monoclonal Antibody, Unconjugated (bsm-33279M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.

Catalog #1: bsm-33279M

Applications: WB, IHC-P, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Unconjugated

Tissue\/cell: Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (Bax) monoclonal Antibody, Unconjugated (bsm-33283M) 1:100, 90 minutes at 37\u00b0C; followed by a CY3 conjugated Goat Anti-Mouse IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
Lane 1: Human 293T cell lysates; Lane 2: Human HT-1080 cell lysates; Lane 3: Human Raji cell lysates; Lane 4: Human SH-SY5Y cell lysates probed with Bax Monoclonal Antibody, Unconjugated (bsm-33283M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bsm-33283m-TR

Applications: WB, ICC/IF

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-33279M-BF350

Applications: WB, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse

Conjugate: AbBy Fluor-350

Catalog #1: bsm-33279M-BF405

Applications: WB, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse

Conjugate: AbBy Fluor-405

Catalog #1: bsm-33279M-BF488

Applications: WB, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse

Conjugate: AbBy Fluor-488

Catalog #1: bsm-33279M-BF555

Applications: WB, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse

Conjugate: AbBy Fluor-555

Catalog #1: bsm-33279M-BF594

Applications: WB, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse

Conjugate: AbBy Fluor-594

Catalog #1: bsm-33279M-BF647

Applications: WB, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse

Conjugate: AbBy Fluor-647

Catalog #1: bsm-33279M-BF680

Applications: WB, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse

Conjugate: AbBy Fluor-680

Catalog #1: bsm-33279M-BF750

Applications: WB, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse

Conjugate: AbBy Fluor-750