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Catalog #1: bsm-0387m-percp-cy7-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human

Conjugate: PerCP-Cy7

Catalog #1: bsm-33207m-percp-cy7-100ul

Applications: WB

Reactivity: Rat

Conjugate: PerCP-Cy7

Catalog #1: bsm-0387m-rbitc-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human

Conjugate: RBITC

Catalog #1: bsm-33207M-RBITC

Applications: WB

Reactivity: Rat

Conjugate: RBITC

Catalog #1: bsm-51175M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51113M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-60273M

Applications: WB

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-51042M

Applications: WB, IHC-P

Reactivity: Human, Mouse

Conjugate: Unconjugated

Blocking buffer: 5% NFDM\/TBST\r\nPrimary Ab dilution: 1:1000\r\nPrimary Ab incubation condition: room temperature 2h\r\nSecondary Ab: Goat Anti-Mouse IgG H&L (HRP)\r\nLysate: 1: HeLa, 2: 293, 3: A20, 4: Rat brain\r\nProtein loading quantity: 20 \u03bcg\r\nExposure time: 10 s\r\nPredicted MW: 38 kDa\r\nObserved MW: 38 kDa
Tissue: Human liver cancer\r\nSection type: Formalin fixed & Paraffin -embedded section\r\nRetrieval method: High temperature and high pressure\r\nRetrieval buffer: Tris\/EDTA buffer, pH 9.0 Primary Ab dilution: 1:100\r\nPrimary Ab incubation condition: 1 hour at room temperature\r\nSecondary Ab: SP Kit(Mouse)(sp-0024)\r\nCounter stain: Hematoxylin (Blue)\r\nComment: Color brown is the positive signal for bsm-60463M
Tissue: Rat kidney\r\nSection type: Formalin fixed & Paraffin -embedded section\r\nRetrieval method: High temperature and high pressure\r\nRetrieval buffer: Tris\/EDTA buffer, pH 9.0 Primary Ab dilution: 1:100\r\nPrimary Ab incubation condition: 1 hour at room temperature\r\nSecondary Ab: SP Kit(Mouse)(sp-0024)\r\nCounter stain: Hematoxylin (Blue)\r\nComment: Color brown is the positive signal for bsm-60463M

Catalog #1: bsm-60463m

Applications: WB, IHC-P, IHC-F, IF, ICC/IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1: MCF-7; Lane 2: Jurkat; Lane 3: PC-12 probed with TOP1 (1C6) Monoclonal Antibody, unconjugated (bsm-51396M) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Hela cells stained with TOP1 (1C6) Monoclonal Antibody, bsm-51396M at 1:25 dilution, followed by Alexa Fluor\u00ae 488 goat anti-mouse lgG (green), compared to mouse IgG1 isotype control (blue).
Lane 1: Molt-4 cell lysates; Lane 2: MCF-7 cell lysates; Lane 3: Siha cell lysates; Lane 4: K562 cell lysates probed with TOP1 (1C6) Monoclonal Antibody, Unconjugated (bsm-51396M) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bsm-51396M

Applications: WB, FCM

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-72436m

Applications: WB, ELISA

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-72437m

Applications: ELISA

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-72435m

Applications: WB, ELISA

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-72440m

Applications: WB, ELISA

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-72439m

Applications: WB, ELISA

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-51191M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-60532m-100ul

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-51240M

Applications: WB

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bsm-51362M

Applications: WB

Reactivity: Human, Mouse

Conjugate: Unconjugated

Blocking buffer: 5% NFDM\/TBST\r\nPrimary ab dilution: 1:1000\r\nPrimary ab incubation condition: 4 \u00b0C overnight\r\nLysate: HeLa, MCF-7, 293, 3T3\r\nProtein loading quantity: 20 \u03bcg\r\nExposure time: 60 s\r\nPredicted MW: 22 kDa\r\nObserved MW: 22 kDa
Tissue: Human colon cancer\r\nSection type: Formalin fixed & Paraffin -embedded section\r\nRetrieval method: High temperature and high pressure\r\nRetrieval buffer: Tris\/EDTA buffer, pH 9.0 Primary ab dilution: 1:100\r\nPrimary ab incubation condition: 1 hour at room temperature\r\nCounter stain: Hematoxylin\r\nComment: Color brown is the positive signal for bsm-60355M

Catalog #1: bsm-60355m

Applications: WB, IHC-P, IHC-F, IF

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bsm-51631m

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51060M

Applications: WB, IF

Reactivity: Human

Conjugate: Unconjugated

HepG2 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20\u2103, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Transferrin(Serum Loading Control) Monoclonal Antibody(bsm-33243M)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
25 ug total protein per lane of various lysates (see on figure) probed with Transferrin monoclonal antibody, unconjugated (bsm-33243M) at 1:5000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Catalog #1: bsm-33243m-100ul

Applications: WB, FCM, IHC-P, IHC-F, IF(IHC-P)

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-33245M

Applications: WB, IHC-P, IHC-F, IF

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-33260M

Applications: WB, ELISA

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-33260M-BF350

Applications: WB

Reactivity: Human

Conjugate: AbBy Fluor-350

Catalog #1: bsm-33243m-bf350-100ul

Applications: WB, FCM, IF(IHC-P)

Reactivity: Human

Conjugate: AbBy Fluor-350

Catalog #1: bsm-33245M-BF350

Applications: WB, IF

Reactivity: Human

Conjugate: AbBy Fluor-350

Catalog #1: bsm-33260M-BF405

Applications: WB

Reactivity: Human

Conjugate: AbBy Fluor-405

Catalog #1: bsm-33243m-bf405-100ul

Applications: WB, FCM, IF(IHC-P)

Reactivity: Human

Conjugate: AbBy Fluor-405

Catalog #1: bsm-33245M-BF405

Applications: WB, IF

Reactivity: Human

Conjugate: AbBy Fluor-405

Catalog #1: bsm-33260M-BF488

Applications: WB

Reactivity: Human

Conjugate: AbBy Fluor-488

Catalog #1: bsm-33243m-bf488-100ul

Applications: WB, FCM, IF(IHC-P)

Reactivity: Human

Conjugate: AbBy Fluor-488

Catalog #1: bsm-33245M-BF488

Applications: WB, IF

Reactivity: Human

Conjugate: AbBy Fluor-488

Catalog #1: bsm-33260M-BF555

Applications: WB

Reactivity: Human

Conjugate: AbBy Fluor-555

Catalog #1: bsm-33243m-bf555-100ul

Applications: WB, FCM, IF(IHC-P)

Reactivity: Human

Conjugate: AbBy Fluor-555

Catalog #1: bsm-33245M-BF555

Applications: WB, IF

Reactivity: Human

Conjugate: AbBy Fluor-555

Catalog #1: bsm-33260M-BF594

Applications: WB

Reactivity: Human

Conjugate: AbBy Fluor-594

Catalog #1: bsm-33243m-bf594-100ul

Applications: WB, FCM, IF(IHC-P)

Reactivity: Human

Conjugate: AbBy Fluor-594

Catalog #1: bsm-33245M-BF594

Applications: WB, IF

Reactivity: Human

Conjugate: AbBy Fluor-594

Catalog #1: bsm-33260M-BF647

Applications: WB

Reactivity: Human

Conjugate: AbBy Fluor-647

Catalog #1: bsm-33243m-bf647-100ul

Applications: WB, FCM, IF(IHC-P)

Reactivity: Human

Conjugate: AbBy Fluor-647

Catalog #1: bsm-33245M-BF647

Applications: WB, IF

Reactivity: Human

Conjugate: AbBy Fluor-647

Catalog #1: bsm-33260M-BF680

Applications: WB

Reactivity: Human

Conjugate: AbBy Fluor-680

Catalog #1: bsm-33243m-bf680-100ul

Applications: WB, FCM, IF(IHC-P)

Reactivity: Human

Conjugate: AbBy Fluor-680

Catalog #1: bsm-33245M-BF680

Applications: WB, IF

Reactivity: Human

Conjugate: AbBy Fluor-680

Catalog #1: bsm-33260M-BF750

Applications: WB

Reactivity: Human

Conjugate: AbBy Fluor-750

Catalog #1: bsm-33243m-bf750-100ul

Applications: WB, FCM, IF(IHC-P)

Reactivity: Human

Conjugate: AbBy Fluor-750