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Paraformaldehyde-fixed, paraffin embedded (mouse lymphoid); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (CD2) Monoclonal Antibody, Unconjugated (bsm-33075M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse spleen); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (CD2) Monoclonal Antibody, Unconjugated (bsm-33075M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.

Catalog #1: bsm-33075m

Applications: FCM

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-33075M-BF350

Applications: FCM

Reactivity:

Conjugate: AbBy Fluor-350

Catalog #1: bsm-33075M-BF405

Applications: FCM

Reactivity:

Conjugate: AbBy Fluor-405

Catalog #1: bsm-33075M-BF488

Applications: FCM

Reactivity:

Conjugate: AbBy Fluor-488

Catalog #1: bsm-33075M-BF555

Applications: FCM

Reactivity:

Conjugate: AbBy Fluor-555

Catalog #1: bsm-33075M-BF594

Applications: FCM

Reactivity:

Conjugate: AbBy Fluor-594

Catalog #1: bsm-33075M-BF647

Applications: FCM

Reactivity:

Conjugate: AbBy Fluor-647

Catalog #1: bsm-33075M-BF680

Applications: FCM

Reactivity:

Conjugate: AbBy Fluor-680

Catalog #1: bsm-33075M-BF750

Applications: FCM

Reactivity:

Conjugate: AbBy Fluor-750

Catalog #1: bsm-33075M-APC

Applications: FCM

Reactivity:

Conjugate: APC

Catalog #1: bsm-33075M-APC-Cy5.5

Applications: FCM

Reactivity:

Conjugate: APC-Cy5.5

Catalog #1: bsm-33075M-APC-Cy7

Applications: FCM

Reactivity:

Conjugate: APC-Cy7

Catalog #1: bsm-33075M-Cy3

Applications: FCM

Reactivity:

Conjugate: Cy3

Catalog #1: bsm-33075M-Cy5

Applications: FCM

Reactivity:

Conjugate: Cy5

Catalog #1: bsm-33075M-Cy5.5

Applications: FCM

Reactivity:

Conjugate: Cy5.5

Catalog #1: bsm-33075M-Cy7

Applications: FCM

Reactivity:

Conjugate: Cy7

Catalog #1: bsm-33075M-FITC

Applications: FCM

Reactivity:

Conjugate: FITC

Catalog #1: bsm-33075M-PE

Applications: FCM

Reactivity:

Conjugate: PE

Catalog #1: bsm-33075M-PE-Cy5

Applications: FCM

Reactivity:

Conjugate: PE-Cy5

Catalog #1: bsm-33075M-PE-Cy5.5

Applications: FCM

Reactivity:

Conjugate: PE-Cy5.5

Catalog #1: bsm-33075M-PE-Cy7

Applications: FCM

Reactivity:

Conjugate: PE-Cy7

Catalog #1: bsm-33075M-PerCP

Applications: FCM

Reactivity:

Conjugate: PerCP

Catalog #1: bsm-33075M-PerCP-Cy5.5

Applications: FCM

Reactivity:

Conjugate: PerCP-Cy5.5

Catalog #1: bsm-33075m-percp-cy7-100ul

Applications: FCM

Reactivity:

Conjugate: PerCP-Cy7

Catalog #1: bsm-33075M-RBITC

Applications: FCM

Reactivity:

Conjugate: RBITC

Catalog #1: bsm-51506m

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

25 ug total protein per lane of various lysates (see on figure) probed with CD20 monoclonal antibody, unconjugated (bsm-33054M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Human Liver Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CD20 Monoclonal Antibody, Unconjugated (bsm-33054M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Diffuse large B-cell lymphoma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CD20 Monoclonal Antibody, Unconjugated (bsm-33054M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CD20 Monoclonal Antibody, Unconjugated (bsm-33054M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.

Catalog #1: bsm-33054m-100ul

Applications: WB, IHC-P, IHC-F, IF(IHC-P)

Reactivity: Human

Conjugate: Unconjugated

The Daudi(H) cells were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.). Primary Antibody (green): Mouse Anti-CD23 antibody (bsm-33088M): 1 \u03bcg\/10^6 cells; Secondary Antibody (white blue): Goat anti-Mouse IgG-FITC (bs-60296G-FITC): 1 \u03bcg\/test.Blank control (black): PBS. Acquisition of 20,000 events was performed.
Paraformaldehyde-fixed, paraffin embedded Human Tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD23 Monoclonal Antibody, Unconjugated(bsm-33088M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Lymph; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD23 Monoclonal Antibody, Unconjugated(bsm-33088M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD23 Monoclonal Antibody, Unconjugated(bsm-33088M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.

Catalog #1: bsm-33088m-100ul

Applications: FCM, IHC-P, IHC-F, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-33088m-bf350-100ul

Applications: FCM, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-350

Catalog #1: bsm-33088m-bf405-100ul

Applications: FCM, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-405

Catalog #1: bsm-33088m-bf488-100ul

Applications: FCM, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-488

Catalog #1: bsm-33088m-bf555-100ul

Applications: FCM, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-555

Catalog #1: bsm-33088m-bf594-100ul

Applications: FCM, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-594

Catalog #1: bsm-33088m-bf647-100ul

Applications: FCM, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-647

Catalog #1: bsm-33088m-bf680-100ul

Applications: FCM, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-680

Catalog #1: bsm-33088m-bf750-100ul

Applications: FCM, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-750

Catalog #1: bsm-33088m-apc-100ul

Applications: FCM, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: APC

Catalog #1: bsm-33088m-apc-Cy5.5-100ul

Applications: FCM, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: APC-Cy5.5

Catalog #1: bsm-33088m-apc-cy7-100ul

Applications: FCM, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: APC-Cy7

Catalog #1: bsm-33088m-biotin-100ul

Applications: IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: Biotin

Catalog #1: bsm-33088m-cy3-100ul

Applications: FCM, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: Cy3

Catalog #1: bsm-33088m-cy5-100ul

Applications: FCM, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: Cy5

Catalog #1: bsm-33088m-Cy5.5-100ul

Applications: FCM, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: Cy5.5

Catalog #1: bsm-33088m-cy7-100ul

Applications: FCM, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: Cy7

Catalog #1: bsm-33088m-fitc-100ul

Applications: FCM, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: FITC

Catalog #1: bsm-33088m-hrp-100ul

Applications: IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: HRP

Catalog #1: bsm-33088m-pe-100ul

Applications: FCM, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: PE

Catalog #1: bsm-33088m-pe-cy5-100ul

Applications: FCM

Reactivity: Human, Mouse, Rat

Conjugate: PE-Cy5