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Catalog #1: bsm-33232m-cy3-100ul

Applications: WB, FCM, IF(IHC-P)

Reactivity: Mouse, Rat

Conjugate: Cy3

Catalog #1: bsm-33234m-cy3-100ul

Applications: FCM, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Cy3

Catalog #1: bsm-33337m-cy3-100ul

Applications: WB, IF

Reactivity: Human, Mouse, Rat

Conjugate: Cy3

Catalog #1: bsm-33232m-cy5-100ul

Applications: WB, FCM, IF(IHC-P)

Reactivity: Mouse, Rat

Conjugate: Cy5

Catalog #1: bsm-33234m-cy5-100ul

Applications: FCM, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Cy5

Catalog #1: bsm-33337m-cy5-100ul

Applications: WB, IF

Reactivity: Human, Mouse, Rat

Conjugate: Cy5

Catalog #1: bsm-33232m-Cy5.5-100ul

Applications: WB, FCM, IF(IHC-P)

Reactivity: Mouse, Rat

Conjugate: Cy5.5

Catalog #1: bsm-33234m-Cy5.5-100ul

Applications: FCM, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Cy5.5

Catalog #1: bsm-33337m-Cy5.5-100ul

Applications: WB, IF

Reactivity: Human, Mouse, Rat

Conjugate: Cy5.5

Catalog #1: bsm-33232m-cy7-100ul

Applications: WB, FCM, IF(IHC-P)

Reactivity: Mouse, Rat

Conjugate: Cy7

Catalog #1: bsm-33234m-cy7-100ul

Applications: FCM, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Cy7

Catalog #1: bsm-33337m-cy7-100ul

Applications: WB, IF

Reactivity: Human, Mouse, Rat

Conjugate: Cy7

Catalog #1: bsm-33232m-fitc-100ul

Applications: WB, FCM, IF(IHC-P)

Reactivity: Mouse, Rat

Conjugate: FITC

Catalog #1: bsm-33234m-fitc-100ul

Applications: FCM, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: FITC

Catalog #1: bsm-33337m-fitc-100ul

Applications: WB, IF

Reactivity: Human, Mouse, Rat

Conjugate: FITC

Catalog #1: bsm-33232m-hrp-100ul

Applications: WB, IHC-P, IHC-F

Reactivity: Mouse, Rat

Conjugate: HRP

Catalog #1: bsm-33234m-hrp-100ul

Applications: IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: HRP

Catalog #1: bsm-33337m-hrp-100ul

Applications: WB, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: HRP

Catalog #1: bsm-33337m-pe-100ul

Applications: WB, IF

Reactivity: Human, Mouse, Rat

Conjugate: PE

Catalog #1: bsm-33232m-pe-100ul

Applications: WB, FCM, IF(IHC-P)

Reactivity: Mouse, Rat

Conjugate: PE

Catalog #1: bsm-33234m-pe-100ul

Applications: FCM, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: PE

Catalog #1: bsm-33337m-pe-cy5-100ul

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: PE-Cy5

Catalog #1: bsm-33232m-pe-cy5-100ul

Applications: WB, FCM

Reactivity: Mouse, Rat

Conjugate: PE-Cy5

Catalog #1: bsm-33234m-pe-cy5-100ul

Applications: FCM

Reactivity: Human, Mouse, Rat

Conjugate: PE-Cy5

Catalog #1: bsm-33337m-pe-Cy5.5-100ul

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: PE-Cy5.5

Catalog #1: bsm-33232m-pe-cy55-100ul

Applications: WB, FCM

Reactivity: Mouse, Rat

Conjugate: PE-Cy5.5

Catalog #1: bsm-33234m-pe-cy55-100ul

Applications: FCM

Reactivity: Human, Mouse, Rat

Conjugate: PE-Cy5.5

Catalog #1: bsm-33337m-pe-cy7-100ul

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: PE-Cy7

Catalog #1: bsm-33232m-pe-cy7-100ul

Applications: WB, FCM

Reactivity: Mouse, Rat

Conjugate: PE-Cy7

Catalog #1: bsm-33234m-pe-cy7-100ul

Applications: FCM

Reactivity: Human, Mouse, Rat

Conjugate: PE-Cy7

Catalog #1: bsm-33232m-percp-100ul

Applications: WB, FCM, IF(IHC-P)

Reactivity: Mouse, Rat

Conjugate: PerCP

Catalog #1: bsm-33234m-percp-100ul

Applications: FCM, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: PerCP

Catalog #1: bsm-33337m-percp-100ul

Applications: WB, IF

Reactivity: Human, Mouse, Rat

Conjugate: PerCP

Catalog #1: bsm-33232m-percp-Cy5.5-100ul

Applications: WB, FCM, IF(IHC-P)

Reactivity: Mouse, Rat

Conjugate: PerCP-Cy5.5

Catalog #1: bsm-33234m-percp-Cy5.5-100ul

Applications: FCM, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: PerCP-Cy5.5

Catalog #1: bsm-33337m-percp-Cy5.5-100ul

Applications: WB, IF

Reactivity: Human, Mouse, Rat

Conjugate: PerCP-Cy5.5

Catalog #1: bsm-33232m-percp-cy7-100ul

Applications: WB, FCM, IF(IHC-P)

Reactivity: Mouse, Rat

Conjugate: PerCP-Cy7

Catalog #1: bsm-33234m-percp-cy7-100ul

Applications: FCM, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: PerCP-Cy7

Catalog #1: bsm-33337m-percp-cy7-100ul

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: PerCP-Cy7

Catalog #1: bsm-33232m-rbitc-100ul

Applications: WB, FCM, IF(IHC-P)

Reactivity: Mouse, Rat

Conjugate: RBITC

Catalog #1: bsm-33234m-rbitc-100ul

Applications: FCM, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: RBITC

Catalog #1: bsm-33337m-rbitc-100ul

Applications: WB, IF

Reactivity: Human, Mouse, Rat

Conjugate: RBITC

25 ug total protein per lane of various lysates (see on figure) probed with ERK2 monoclonal antibody, unconjugated (bsm-51555M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
The Hela (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.).Primary Antibody (green):Mouse Anti-ERK2 antibody (bsm-51555M): 1 \u03bcg\/10^6 cells; Secondary Antibody (white blue): Goat anti-Mouse IgG-BF488 (bs-60296G-BF488): 1 \u03bcg\/test. Blank control (black): PBS. Acquisition of 20,000 events was performed.

Catalog #1: bsm-51555m-100ul

Applications: WB, FCM

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-60388m

Applications: WB, ICC/IF

Reactivity: Human

Conjugate: Unconjugated

Blocking buffer: 5% NFDM\/TBST\r\nPrimary ab dilution: 1\uff1a1000\r\nPrimary ab incubation condition: overnight, at 4 degree\r\nSecondary ab: Goat Anti-Mouse IgG H&L (HRP)\r\nLysate: HeLa, 293, 4T1, Raw264.7\r\nProtein loading quantity: 20 \u03bcg\r\nExposure time: 30 s\r\nPredicted MW: 29 kDa\r\nObserved MW: 29 kDa
Tissue: Human liver\r\nSection type: Formalin fixed & Paraffin -embedded section\r\nRetrieval method: High temperature and high pressure\r\nRetrieval buffer: Tris\/EDTA buffer, pH 9.0 Primary ab dilution: 1:100\r\nPrimary ab incubation condition: 1 hour at room temperature\r\nSecondary ab: SP Kit(Mouse)(sp-0024)\r\nCounter stain: Hematoxylin (Blue)\r\nComment: Color brown is the positive signal for bsm-60396M

Catalog #1: bsm-60396m

Applications: WB, IHC-P, IHC-F, IF, ICC/IF

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bsm-42106m-100ul

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-42106m-bf350-100ul

Applications: WB

Reactivity: Human

Conjugate: AbBy Fluor-350

Catalog #1: bsm-42106m-bf405-100ul

Applications: WB

Reactivity: Human

Conjugate: AbBy Fluor-405