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Lane 1 : Bosc23 cell lysate transfected with HA\/HSP60; Lane 2 : Bosc23 cell lysate transfected with Myc\/His\/HePTP; Lane 3 : Bosc23 cell lysate transfected with GFP\/Ub+1 lysates, probed with HA tag (7H5) Monoclonal Antibody, unconjugated (bsm-50131M) at 1:10000 overnight at 4\u00b0C followed by a conjugated secondary antibody at 1:5000 for 60 minutes at Room Temperature.
Immunoprecipitation of Bosc23 cell lysates using HA tag (7H5) \r\nMonoclonal Antibody (bsm-50131M): Lane 1 : Bosc23 cell lysate transfected with HA\/HSP60; Lane 2: Precipitated from 200ug Bosc23 transfected with HA\/HSP60 using 5ug of antibody; Lane 3 : Precipitated from PBS using 5ug of antibody; WB analysis was performed using bsm-50131M at 0.1ug\/mL (1:10000) and incubated at 4\u2103 overnight, followed secondary antibody incubation for 60min at Room Temperature.

Catalog #1: bsm-50131M

Applications: WB, IF(ICC), IP

Reactivity: Human, All

Conjugate: Unconjugated

Catalog #1: bs-51024R

Applications: WB, IF

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51205M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-72168m

Applications: ELISA

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-72169m

Applications: ELISA

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-72170m

Applications: ELISA

Reactivity:

Conjugate: Unconjugated

Lane 1: THP-1 Cell lysates; Lane 2: HL-60 Cell lysates; probed with HCK (8C1) Monoclonal Antibody, unconjugated (bsm-51307M) at 1:2000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HepG2 cells labeled with HCK (8C1) Monoclonal Antibody (bsm-51307M) at 1\/25 dilution, followed by secondary antibody (green), cytoplasmic actin staining (red), and nuclear counter stain with DAPI (blue).

Catalog #1: bsm-51307M

Applications: WB, IF(ICC), IF

Reactivity:

Conjugate: Unconjugated

Whole cell extracts (40 \u03bcg) from HeLa cells transfected with HDAC2 siRNA (lane 2) and from an untransfected control (lane 1) were analysed by Western blot using HDAC2 (7G5) Monoclonal Antibody (bsm-53026M) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right (expected size: 55 kDa); the marker (in kDa) is shown on the left.
HeLa cells were stained with HDAC2 (7G5) Monoclonal Antibody (bsm-53026M) and with DAPI. Cells were fixed with 4% formaldehyde for 10\u2019 and blocked with PBS\/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labelled with the HDAC2 antibody (left) diluted 1:500 in blocking solution followed by an anti-mouse antibody conjugated to Alexa594. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.
ChIP assays were performed using HeLa cells, HDAC2 (7G5) Monoclonal Antibody (bsm-53026M) and optimized PCR primer sets for qPCR. ChIP was performed using sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2 and 5 \u03bcg per ChIP experiment was analysed. IgG (2 \u03bcg\/IP) was used as negative IP control. QPCR was performed with primers for the ACTB, EIF4A2 and LMO4 genes, used as positive controls, and for the Sat2 satellite repeat, used as negative control. Figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).
ChIP was performed on sheared chromatin from 4 million HeLa cells using 1 \u03bcg of HDAC2 (7G5) Monoclonal Antibody (Cat. No. C15200201) as described above. The IP\u2019d DNA was subsequently analyzed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer\u2019s instructions. The 51 bp tags were aligned to the mouse genome using the BWA algorithm. Figure shows the enrichment along the complete sequence and a 2 Mb region of human chromosome 3 (fig 2A and B), and in a two genomic regions surrounding the LMO4 positive control gene and the MAGEC1 gene (figure 2C and D).

Catalog #1: bsm-53026M

Applications: WB, ELISA, IF(ICC), dot-blot, ChIP-seq

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-51026R

Applications: WB, IF

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-72171m

Applications: WB, ELISA

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-72172m

Applications: WB, ELISA

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-72173m

Applications: WB, ELISA

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-72179m

Applications: WB, ELISA

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-72180m

Applications: WB, ELISA

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-72177m

Applications: ELISA

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-72178m

Applications: WB, ELISA

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-72185m

Applications: ELISA

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-72194m

Applications: ELISA

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-72198m

Applications: ELISA, IHC

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-72200m

Applications: WB, ELISA

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-72197m

Applications: ELISA

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-72204m

Applications: ELISA

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-72202m

Applications: WB, ELISA

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-72205m

Applications: WB, ELISA

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-72206m

Applications: WB, ELISA

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-72207m

Applications: WB, ELISA

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-72208m

Applications: WB, ELISA

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-72210m

Applications: WB, ELISA

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-72211m

Applications: WB, ELISA

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-72216m

Applications: WB, ELISA

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-72217m

Applications: WB, ELISA

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-72215m

Applications: WB, ELISA

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-72218m

Applications: WB, ELISA

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-72219m

Applications: WB, ELISA

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-72220m

Applications: WB, ELISA

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-72221m

Applications: WB, ELISA

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-72222m

Applications: WB, ELISA, IHC

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-72223m

Applications: ELISA

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-72225m

Applications: ELISA

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-72229m

Applications: ELISA

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-72231m

Applications: ELISA, IHC

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-72230m

Applications: ELISA, IHC

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-72235m

Applications: ELISA

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-72237m

Applications: ELISA

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-72238m

Applications: ELISA

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-72239m

Applications: ELISA

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-51153M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Hep-G2 (H) cells were treated with or without CoCl2 (500uM) for 6 h, 25 \u03bcg total protein per lane of cell lysates (see on figure) probed with HIF-1 Alpha monoclonal antibody, unconjugated (bsm-33428M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
4% Paraformaldehyde-fixed HepG2\uff08treated with 1mM CoCl2 for 24 hours\uff09 (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (HIF-1a) monoclonal Antibody, unconjugated (bsm-33428M) 1:100, 90 min at 37\u00b0C; followed by conjugated Goat Anti-Mouse IgG antibody (green, bs-60296G-BF488) at 37\u00b0C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.
4% Paraformaldehyde-fixed Hela\uff08treated with 1mM CoCl2 for 24 hours\uff09 (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (HIF-1a) monoclonal Antibody, unconjugated (bsm-33428M) 1:100, 90 min at 37\u00b0C; followed by conjugated Goat Anti-Mouse IgG antibody (green, bs-60296G-BF488) at 37\u00b0C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.
The Hela (treated with 500uM CoCl2 for 6 hours) (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.).Primary Antibody (green):Mouse Anti-HIF-1 Alpha antibody (bsm-33428M): 1 \u03bcg\/10^6 cells; Secondary Antibody (white blue): Goat anti-Mouse IgG-BF488 (bs-60296G-BF488): 1 \u03bcg\/test. Blank control (black): PBS. Acquisition of 20,000 events was performed.

Catalog #1: bsm-33428m-100ul

Applications: WB, FCM, IF(ICC)

Reactivity: Human

Conjugate: Unconjugated