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Catalog #1: bsm-72202m

Applications: WB, ELISA

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-72205m

Applications: WB, ELISA

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-72206m

Applications: WB, ELISA

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-72207m

Applications: WB, ELISA

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-72208m

Applications: WB, ELISA

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-72210m

Applications: WB, ELISA

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-72211m

Applications: WB, ELISA

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-72216m

Applications: WB, ELISA

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-72217m

Applications: WB, ELISA

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-72215m

Applications: WB, ELISA

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-72218m

Applications: WB, ELISA

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-72219m

Applications: WB, ELISA

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-72220m

Applications: WB, ELISA

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-72221m

Applications: WB, ELISA

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-72222m

Applications: WB, ELISA, IHC

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-72223m

Applications: ELISA

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-72225m

Applications: ELISA

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-72229m

Applications: ELISA

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-72231m

Applications: ELISA, IHC

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-72230m

Applications: ELISA, IHC

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-72235m

Applications: ELISA

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-72237m

Applications: ELISA

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-72238m

Applications: ELISA

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-72239m

Applications: ELISA

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-51153M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Hep-G2 (H) cells were treated with or without CoCl2 (500uM) for 6 h, 25 \u03bcg total protein per lane of cell lysates (see on figure) probed with HIF-1 Alpha monoclonal antibody, unconjugated (bsm-33428M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
4% Paraformaldehyde-fixed HepG2\uff08treated with 1mM CoCl2 for 24 hours\uff09 (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (HIF-1a) monoclonal Antibody, unconjugated (bsm-33428M) 1:100, 90 min at 37\u00b0C; followed by conjugated Goat Anti-Mouse IgG antibody (green, bs-60296G-BF488) at 37\u00b0C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.
4% Paraformaldehyde-fixed Hela\uff08treated with 1mM CoCl2 for 24 hours\uff09 (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (HIF-1a) monoclonal Antibody, unconjugated (bsm-33428M) 1:100, 90 min at 37\u00b0C; followed by conjugated Goat Anti-Mouse IgG antibody (green, bs-60296G-BF488) at 37\u00b0C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.
The Hela (treated with 500uM CoCl2 for 6 hours) (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.).Primary Antibody (green):Mouse Anti-HIF-1 Alpha antibody (bsm-33428M): 1 \u03bcg\/10^6 cells; Secondary Antibody (white blue): Goat anti-Mouse IgG-BF488 (bs-60296G-BF488): 1 \u03bcg\/test. Blank control (black): PBS. Acquisition of 20,000 events was performed.

Catalog #1: bsm-33428m

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-33428m-bf350-100ul

Applications: WB

Reactivity: Human

Conjugate: AbBy Fluor-350

Catalog #1: bsm-33428m-bf405-100ul

Applications: WB

Reactivity: Human

Conjugate: AbBy Fluor-405

Catalog #1: bsm-33428m-bf488-100ul

Applications: WB

Reactivity: Human

Conjugate: AbBy Fluor-488

Catalog #1: bsm-33428m-bf555-100ul

Applications: WB

Reactivity: Human

Conjugate: AbBy Fluor-555

Catalog #1: bsm-33428m-bf594-100ul

Applications: WB

Reactivity: Human

Conjugate: AbBy Fluor-594

Catalog #1: bsm-33428m-bf647-100ul

Applications: WB

Reactivity: Human

Conjugate: AbBy Fluor-647

Catalog #1: bsm-33428m-bf680-100ul

Applications: WB

Reactivity: Human

Conjugate: AbBy Fluor-680

Catalog #1: bsm-33428m-bf750-100ul

Applications: WB

Reactivity: Human

Conjugate: AbBy Fluor-750

Catalog #1: bsm-33428m-apc-100ul

Applications: WB

Reactivity: Human

Conjugate: APC

Catalog #1: bsm-33428m-apc-Cy5.5-100ul

Applications: WB

Reactivity: Human

Conjugate: APC-Cy5.5

Catalog #1: bsm-33428m-apc-cy7-100ul

Applications: WB

Reactivity: Human

Conjugate: APC-Cy7

Catalog #1: bsm-33428m-biotin-100ul

Applications: WB

Reactivity: Human

Conjugate: Biotin

Catalog #1: bsm-33428m-cy3-100ul

Applications: WB

Reactivity: Human

Conjugate: Cy3

Catalog #1: bsm-33428m-cy5-100ul

Applications: WB

Reactivity: Human

Conjugate: Cy5

Catalog #1: bsm-33428m-Cy5.5-100ul

Applications: WB

Reactivity: Human

Conjugate: Cy5.5

Catalog #1: bsm-33428m-cy7-100ul

Applications: WB

Reactivity: Human

Conjugate: Cy7

Catalog #1: bsm-33428m-fitc-100ul

Applications: WB

Reactivity: Human

Conjugate: FITC

Catalog #1: bsm-33428m-hrp-100ul

Applications: WB

Reactivity: Human

Conjugate: HRP

Catalog #1: bsm-33428m-pe-100ul

Applications: WB

Reactivity: Human

Conjugate: PE

Catalog #1: bsm-33428m-pe-cy5-100ul

Applications: WB

Reactivity: Human

Conjugate: PE-Cy5

Catalog #1: bsm-33428m-pe-Cy5.5-100ul

Applications: WB

Reactivity: Human

Conjugate: PE-Cy5.5

Catalog #1: bsm-33428m-pe-cy7-100ul

Applications: WB

Reactivity: Human

Conjugate: PE-Cy7