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Paraformaldehyde-fixed, paraffin embedded human hepatocellular carcinoma; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with Serum albumin (3F4) Monoclonal Antibody, Unconjugated (bsm-0945M) at 1:500 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.
Lane 1:Human Serum probed with Serum albumin Monoclonal Antibody, Unconjugated (bsm-0945M) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded (human liver carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (Human Serum albumin (Loading Control)) Monoclonal Antibody, Unconjugated (bsm-0945M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.

Catalog #1: bsm-0945m

Applications: WB, IHC-P, IF(IHC-P)

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bsm-0945m-bf350-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse

Conjugate: AbBy Fluor-350

Catalog #1: bsm-0945m-bf405-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse

Conjugate: AbBy Fluor-405

Catalog #1: bsm-0945m-bf488-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse

Conjugate: AbBy Fluor-488

Catalog #1: bsm-0945m-bf555-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse

Conjugate: AbBy Fluor-555

Catalog #1: bsm-0945m-bf594-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse

Conjugate: AbBy Fluor-594

Catalog #1: bsm-0945m-bf647-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse

Conjugate: AbBy Fluor-647

Catalog #1: bsm-0945m-bf680-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse

Conjugate: AbBy Fluor-680

Catalog #1: bsm-0945m-bf750-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse

Conjugate: AbBy Fluor-750

Catalog #1: bsm-0945m-apc-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse

Conjugate: APC

Catalog #1: bsm-0945m-apc-Cy5.5-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse

Conjugate: APC-Cy5.5

Catalog #1: bsm-0945m-apc-cy7-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse

Conjugate: APC-Cy7

Catalog #1: bsm-0945m-biotin-100ul

Applications: WB, IHC-P

Reactivity: Human, Mouse

Conjugate: Biotin

Catalog #1: bsm-0945m-cy3-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse

Conjugate: Cy3

Catalog #1: bsm-0945m-cy5-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse

Conjugate: Cy5

Catalog #1: bsm-0945m-Cy5.5-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse

Conjugate: Cy5.5

Catalog #1: bsm-0945m-cy7-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse

Conjugate: Cy7

Catalog #1: bsm-0945m-fitc-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse

Conjugate: FITC

Catalog #1: bsm-0945m-hrp-100ul

Applications: WB, IHC-P

Reactivity: Human, Mouse

Conjugate: HRP

Catalog #1: bsm-0945m-pe-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse

Conjugate: PE

Catalog #1: bsm-0945m-pe-cy5-100ul

Applications: WB

Reactivity: Human, Mouse

Conjugate: PE-Cy5

Catalog #1: bsm-0945m-pe-Cy5.5-100ul

Applications: WB

Reactivity: Human, Mouse

Conjugate: PE-Cy5.5

Catalog #1: bsm-0945m-pe-cy7-100ul

Applications: WB

Reactivity: Human, Mouse

Conjugate: PE-Cy7

Catalog #1: bsm-0945m-percp-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse

Conjugate: PerCP

Catalog #1: bsm-0945m-percp-Cy5.5-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse

Conjugate: PerCP-Cy5.5

Catalog #1: bsm-0945m-percp-cy7-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse

Conjugate: PerCP-Cy7

Catalog #1: bsm-0945m-rbitc-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse

Conjugate: RBITC

Catalog #1: bsm-30227m-fitc-100t

Applications: FCM

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-30227m-pe-100t

Applications: FCM

Reactivity: Human

Conjugate: Unconjugated

Lane 1: HeLa Cell lysates; Lane 2: MCF-7 Cell lysates; Lane 3: K562 Cell lysates; Lane 4: NIH\/3T3 Cell lysates; Lane 5: PC-12 Cell lysates; probed with IF4E (4C5) Monoclonal Antibody, unconjugated (bsm-51305M) at 1:4000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized MCF-7 cells labeled with IF4E (4C5) Monoclonal Antibody (bsm-51305M) at 1\/25 dilution, followed by secondary antibody (green), cytoplasmic actin staining (red), and nuclear counter stain with DAPI (blue).

Catalog #1: bsm-51305M

Applications: WB, IF(ICC), IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded (human spleen); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (IFI16) Monoclonal Antibody, Unconjugated (bsm-60844M) at 1:300 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human tonsil); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (IFI16) Monoclonal Antibody, Unconjugated (bsm-60844M) at 1:300 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (IFI16) Monoclonal Antibody, Unconjugated (bsm-60844M) at 1:300 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human tonsils; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with IFI16 Monoclonal Antibody, Unconjugated (bsm-60844M) at 1:200 overnight at 4\u00b0C. Followed by conjugated Goat Anti-Mouse IgG antibody (red, bs-0296G-BF594), DAPI (blue, C02-04002) was used to stain the cell nuclei.

Catalog #1: bsm-60844m-100ul

Applications: IHC-P, IHC-F, IF(IHC-P)

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51383M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51550m

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51186M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51184M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-60284M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

SH-SY5Y lysates (20ug) probed with INA (257CT7.1.2) Monoclonal Antibody, unconjugated (bsm-51074M) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Mouse brain cell lysates; probed with INA (257CT7.1.2) Monoclonal Antibody, unconjugated (bsm-51074M) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

Catalog #1: bsm-51074M

Applications: WB

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bsm-72267m

Applications: ELISA

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-72266m

Applications: ELISA

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-72268m

Applications: ELISA

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-70525M

Applications: WB, IP

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-70527M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-70524M

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa cells labeled with IRF3 (2C4) Monoclonal Antibody (bsm-51312M) at 1\/25 dilution, followed by secondary antibody (green), cytoplasmic actin staining (red), and nuclear counter stain with DAPI (blue).
Jurkat cells probed with using IRF3 (2C4) Monoclonal Antibody bsm-51312M at 1:25 for 60 minutes at 37\u00baC followed by secondary antibody at 1:400 (green) compared to an isotype control of mouse IgG (blue).
Lane 1: COS-7; Lane 2: Daudi; Lane 3: HepG2; Lane 4: HT-29; Lane 5: Jurkat; Lane 6: MCF-7; probed with IRF3 (2C4) Polyclonal Antibody, unconjugated (bsm-51312M) at 1:2000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Paraformaldehyde-fixed, paraffin embedded human kidney tissue; Blocked with 3% BSA for 30min at RT; Antibody incubation with IRF3 (2C4) Monoclonal Antibody, Unconjugated (bsm-51312M) at 1:25 for 1hr at 37\u00b0C, followed by a conjugated secondary antibody and DAB staining.

Catalog #1: bsm-51312M

Applications: WB, FCM, IHC-P, IF(ICC), IF

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-51373m-100ul

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51027M

Applications: WB, FCM, IHC-P, IF

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51250M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Lane 1: HL-60; Lane 2: K562 cell lysate at 20 \u00b5g per lane, probed with bsm-51424M ITGB2 (534CT6.2.1) Monoclonal Antibody at 1:1000 dilution and 4\u2103 overnight incubation, followed by secondary antibody incubation for 60min at room temperature.
Paraformaldehyde-fixed, paraffin embedded human tonsil tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 15 minutes; Blocking buffer (3% BSA) at room temperature for 30min; Antibody incubation with ITGB2 (534CT6.2.1) Monoclonal Antibody (bsm-51424M) at 1:25 for 1 hour at 37\u00b0C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.

Catalog #1: bsm-51424M

Applications: WB, IHC-P

Reactivity: Human

Conjugate: Unconjugated