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Catalog #1: bsm-33417m-pe-Cy5.5-100ul

Applications: WB, Flow-Cyt

Reactivity: Human

Conjugate: PE-Cy5.5

Catalog #1: bsm-33417m-pe-cy7-100ul

Applications: WB, Flow-Cyt

Reactivity: Human

Conjugate: PE-Cy7

Catalog #1: bsm-33417m-percp-100ul

Applications: WB, Flow-Cyt

Reactivity: Human

Conjugate: PerCP

Catalog #1: bsm-33417m-percp-Cy5.5-100ul

Applications: WB, Flow-Cyt

Reactivity: Human

Conjugate: PerCP-Cy5.5

Catalog #1: bsm-33417m-percp-cy7-100ul

Applications: WB

Reactivity: Human

Conjugate: PerCP-Cy7

Catalog #1: bsm-33417m-rbitc-100ul

Applications: WB, Flow-Cyt

Reactivity: Human

Conjugate: RBITC

4% Paraformaldehyde-fixed Hela (treated with Sodium Butyrate (5mM, 24h)) (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (Methylated Lysine) monoclonal Antibody, unconjugated (bsm-33197M) 1:100, 90 min at 37\u00b0C; followed by conjugated Goat Anti-Mouse IgG antibody (green, bs-60296G-FITC) at 37\u00b0C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.
The Hela (treated with Sodium Butyrate (5mM, 24h)) (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.).Primary Antibody (green):Mouse Anti-Methylated Lysine antibody (bsm-33187M): 1 \u03bcg\/10^6 cells; Secondary Antibody (white blue): Goat anti-Mouse IgG-FITC (bs-60296G-FITC): 1 \u03bcg\/test. Blank control (black): PBS. Acquisition of 20,000 events was performed.

Catalog #1: bsm-33197m

Applications: WB, IHC-P, IHC-F, IF, Flow-Cyt, ICC/IF

Reactivity: Human, Species independent

Conjugate: Unconjugated

Catalog #1: bsm-33197m-bf350-100ul

Applications: WB, IF, Flow-Cyt

Reactivity: Human, Species independent

Conjugate: AbBy Fluor-350

Catalog #1: bsm-33197m-bf405-100ul

Applications: WB, IF, Flow-Cyt

Reactivity: Human, Species independent

Conjugate: AbBy Fluor-405

Catalog #1: bsm-33197m-bf488-100ul

Applications: WB, IF, Flow-Cyt

Reactivity: Human, Species independent

Conjugate: AbBy Fluor-488

Catalog #1: bsm-33197m-bf555-100ul

Applications: WB, IF, Flow-Cyt

Reactivity: Human, Species independent

Conjugate: AbBy Fluor-555

Catalog #1: bsm-33197m-bf594-100ul

Applications: WB, IF, Flow-Cyt

Reactivity: Human, Species independent

Conjugate: AbBy Fluor-594

Catalog #1: bsm-33197m-bf647-100ul

Applications: WB, IF

Reactivity: Human, Species independent

Conjugate: AbBy Fluor-647

Catalog #1: bsm-33197m-bf680-100ul

Applications: WB, IF

Reactivity: Human, Species independent

Conjugate: AbBy Fluor-680

Catalog #1: bsm-33197m-bf750-100ul

Applications: WB, IF, Flow-Cyt

Reactivity: Human, Species independent

Conjugate: AbBy Fluor-750

Catalog #1: bsm-33197m-apc-100ul

Applications: WB, IF, Flow-Cyt

Reactivity: Human, Species independent

Conjugate: APC

Catalog #1: bsm-33197m-apc-Cy5.5-100ul

Applications: WB, IF, Flow-Cyt

Reactivity: Human, Species independent

Conjugate: APC-Cy5.5

Catalog #1: bsm-33197m-apc-cy7-100ul

Applications: WB, Flow-Cyt

Reactivity: Human, Species independent

Conjugate: APC-Cy7

Catalog #1: bsm-33197m-biotin-100ul

Applications: WB, IHC-P, IHC-F

Reactivity: Human, Species independent

Conjugate: Biotin

Catalog #1: bsm-33197m-cy3-100ul

Applications: WB, IF, Flow-Cyt

Reactivity: Human, Species independent

Conjugate: Cy3

Catalog #1: bsm-33197m-cy5-100ul

Applications: WB, IF, Flow-Cyt

Reactivity: Human, Species independent

Conjugate: Cy5

Catalog #1: bsm-33197m-Cy5.5-100ul

Applications: WB, IF, Flow-Cyt

Reactivity: Human, Species independent

Conjugate: Cy5.5

Catalog #1: bsm-33197m-cy7-100ul

Applications: WB, IF, Flow-Cyt

Reactivity: Human, Species independent

Conjugate: Cy7

Catalog #1: bsm-33197m-fitc-100ul

Applications: WB, IF, Flow-Cyt

Reactivity: Human, Species independent

Conjugate: FITC

Catalog #1: bsm-33197m-hrp-100ul

Applications: WB, IHC-P, IHC-F

Reactivity: Human, Species independent

Conjugate: HRP

Catalog #1: bsm-33197m-pe-100ul

Applications: WB, IF, Flow-Cyt

Reactivity: Human, Species independent

Conjugate: PE

Catalog #1: bsm-33197m-pe-cy5-100ul

Applications: WB, Flow-Cyt

Reactivity: Human, Species independent

Conjugate: PE-Cy5

Catalog #1: bsm-33197m-pe-Cy5.5-100ul

Applications: WB, Flow-Cyt

Reactivity: Human, Species independent

Conjugate: PE-Cy5.5

Catalog #1: bsm-33197m-pe-cy7-100ul

Applications: WB, Flow-Cyt

Reactivity: Human, Species independent

Conjugate: PE-Cy7

Catalog #1: bsm-33197m-percp-100ul

Applications: WB, IF, Flow-Cyt

Reactivity: Human, Species independent

Conjugate: PerCP

Catalog #1: bsm-33197m-percp-Cy5.5-100ul

Applications: WB, IF, Flow-Cyt

Reactivity: Human, Species independent

Conjugate: PerCP-Cy5.5

Catalog #1: bsm-33197m-percp-cy7-100ul

Applications: WB

Reactivity: Human, Species independent

Conjugate: PerCP-Cy7

Catalog #1: bsm-33197m-rbitc-100ul

Applications: WB, IF, Flow-Cyt

Reactivity: Human, Species independent

Conjugate: RBITC

Catalog #1: bsm-34258m

Applications: ELISA, IHC-P, IHC-F, IF

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-72364m

Applications: ELISA

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-70215M

Applications: WB, IHC

Reactivity: Human, Mouse, Rat, Bovine

Conjugate: Unconjugated

Catalog #1: bsm-72368m

Applications: ELISA

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-70592M

Applications: WB, IP

Reactivity: Human, Mouse, Rat, Chicken

Conjugate: Unconjugated

Catalog #1: bsm-51356M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Human Tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PD-1 Monoclonal Antibody, Unconjugated(bsm-41117M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the SP Kit (Mouse, sp-0024) and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Spleen\r\n; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PD-1 Monoclonal Antibody, Unconjugated(bsm-41117M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the SP Kit (Mouse, sp-0024) and DAB (C-0010) staining.

Catalog #1: bsm-41117m

Applications: IHC-P, IHC-F, IF

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-70603M

Applications: WB, IP

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-70604M

Applications: WB, IP

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51098M

Applications: WB

Reactivity: Mouse

Conjugate: Unconjugated

Catalog #1: bsm-51338M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-70600M

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Human Lung Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PDK2 Monoclonal Antibody, Unconjugated (bsm-51043M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PDK2 Monoclonal Antibody, Unconjugated (bsm-51043M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PDK2 Monoclonal Antibody, Unconjugated (bsm-51043M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
25 ug total protein per lane of various lysates (see on figure) probed with PDK2 monoclonal antibody, unconjugated (bsm-51043M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Catalog #1: bsm-51043m

Applications: WB, IHC-P, IHC-F, IF

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bsm-51108M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded (human lung carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (CD31) Monoclonal Antibody, Unconjugated (bsm-56214M) at 1:150 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (CD31) Monoclonal Antibody, Unconjugated (bsm-56214M) at 1:150 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human liver carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (CD31) Monoclonal Antibody, Unconjugated (bsm-56214M) at 1:150 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human liver ); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (CD31) Monoclonal Antibody, Unconjugated (bsm-56214M) at 1:150 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.

Catalog #1: bsm-56214m

Applications: IHC-P, IHC-F, IF

Reactivity: Rat

Conjugate: Unconjugated