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Catalog #1: bsm-70691M

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-70277M

Applications: WB, IHC

Reactivity: Human, Rat, Pig, Hamster

Conjugate: Unconjugated

Catalog #1: bsm-70692M

Applications: WB, IP

Reactivity: Human, Mouse, Rat, Chicken, Xenopus

Conjugate: Unconjugated

Lane 1: CCRF-CEM cell lysate (20ug); Lane 2: HeLa cell lysate (20ug) probed with bsm-51009M TBB1 (6A4) Monoclonal Antibody at 1:1000 dilution and 4\u2103 overnight incubation, followed by secondary antibody incubation for 60min at room temperature.
Paraformaldehyde-fixed, paraffin embedded Human Brain tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 15 minutes; Blocking buffer (3% BSA) at room temperature for 30min; Antibody incubation with TBB1 (6A4) Monoclonal Antibody, Unconjugated (bs-51009R) at 1:10 for 1 hour at 37\u00b0C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.
HepG2 cells stained with TBB5 (7D3) Monoclonal Antibody (bsm-51387M) at 1:25 dilution, followed by Alexa Fluor 488-conjugated secondary antibody (green), nuclear staining (blue).

Catalog #1: bsm-51009M

Applications: WB, IHC-P

Reactivity: Human, Mouse

Conjugate: Unconjugated

Whole cell extracts (40 \u03bcg) from HeLa cells from an untransfected control (lane 1) and transfected with TBP siRNA (lane 2), and were analysed by Western blot using TBP Monoclonal Antibody (bsm-53003M) diluted 1:500 in TBSTween containing 5% skimmed milk, followed by secondary antibody.
ChIP assays were performed using U2OS cells, TBP (2C9) Monoclonal Antibody (bsm-53003M) and optimized primer sets for qPCR. Sheared chromatin from 1x10e6 cells and 4 \u03bcg of antibody were used per ChIP experiment. QPCR was performed with primers for the promoter of the c-fos and GAPDH genes, a region 0.5 and 1 kb upstream of the GAPDH promoter, and exon 2 of the myoglobin gene as a negative control. Figure shows the recovery (the relative amount of immunoprecipitated DNA compared to input DNA) and the occupancy (ratio +\/- control target). These results demonstrate the occupancy of both promoters by TBP.

Catalog #1: bsm-53003M

Applications: WB, ChIP-seq

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-51003R

Applications: WB

Reactivity: Human, Mouse

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded mouse brain tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 15 minutes; Blocking buffer (3% BSA) at room temperature for 30min; Antibody incubation with TBP (8C4) Monoclonal Antibody (bsm-51220M) at 1:25 for 1 hour at 37\u00b0C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded human testis tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 15 minutes; Blocking buffer (3% BSA) at room temperature for 30min; Antibody incubation with TBP (8C4) Monoclonal Antibody (bsm-51220M) at 1:25 for 1 hour at 37\u00b0C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.
Lane 1: HeLa; Lane 2: HepG2; Lane 3: HIN\/3T3 cell lysate probed with TBP (8C4) Monoclonal Antibody (bsm-51220M) at 1:1000 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at 37˚C.

Catalog #1: bsm-51220M

Applications: WB, IHC-P, IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded human testis tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 15 minutes; Blocking buffer (3% BSA) at room temperature for 30min; Antibody incubation with TERF2IP (4C8) Monoclonal Antibody (bsm-51368M) at 1:25 for 1 hour at 37\u00b0C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.
Lane 1: HepG2 cell lysates; Lane 2: A431 cell lysates; probed with TERF2IP (4C8) Polyclonal Antibody, unconjugated (bsm-51368M) at 1:2000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

Catalog #1: bsm-51368M

Applications: WB, FCM, IHC-P

Reactivity: Human

Conjugate: Unconjugated

Whole cell extracts from HL60 and NB4 cells (40 \u03bcg) were analysed by Western blot using TET2 (6A1) Monoclonal Antibody (bsm-53017M), diluted 1:2,000 in PBS containing 10% milk. The position of the protein of interest (expected MW 224 kDa) is indicated on the right; the marker (in kDa) is shown on the left.
IP was performed on 250 \u03bcg HL60 RIPA cell lysate using TET2 (6A1) Monoclonal Antibody (bsm-53017M) (lane 3) or an IgG negative control (lane 2). The input sample (25 \u03bcg RIPA lysate) was used as a positive control (lane 1). The samples were analysed by Western blot using TET2 (6A1) Monoclonal Antibody (bsm-53017M), diluted 1:2,000 in PBS containing 10% milk.

Catalog #1: bsm-53017M

Applications: WB, IP

Reactivity: Human, Mouse

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded (rat spleen); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (TGF beta 1 ) Monoclonal Antibody, Unconjugated (bsm-33345M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (TGF beta 1 ) Monoclonal Antibody, Unconjugated (bsm-33345M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat intestine); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (TGF beta 1 ) Monoclonal Antibody, Unconjugated (bsm-33345M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse intestine); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (TGF beta 1 ) Monoclonal Antibody, Unconjugated (bsm-33345M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.

Catalog #1: bsm-33345m-100ul

Applications: IHC-P, IHC-F, IF(IHC-P)

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-33345m-bf350-100ul

Applications: IF(IHC-P)

Reactivity: Mouse, Rat

Conjugate: AbBy Fluor-350

Catalog #1: bsm-33345m-bf405-100ul

Applications: IF(IHC-P)

Reactivity: Mouse, Rat

Conjugate: AbBy Fluor-405

Catalog #1: bsm-33345m-bf488-100ul

Applications: IF(IHC-P)

Reactivity: Mouse, Rat

Conjugate: AbBy Fluor-488

Catalog #1: bsm-33345m-bf555-100ul

Applications: IF(IHC-P)

Reactivity: Mouse, Rat

Conjugate: AbBy Fluor-555

Catalog #1: bsm-33345m-bf594-100ul

Applications: IF(IHC-P)

Reactivity: Mouse, Rat

Conjugate: AbBy Fluor-594

Catalog #1: bsm-33345m-bf647-100ul

Applications: IF(IHC-P)

Reactivity: Mouse, Rat

Conjugate: AbBy Fluor-647

Catalog #1: bsm-33345m-bf680-100ul

Applications: IF(IHC-P)

Reactivity: Mouse, Rat

Conjugate: AbBy Fluor-680

Catalog #1: bsm-33345m-bf750-100ul

Applications: IF(IHC-P)

Reactivity: Mouse, Rat

Conjugate: AbBy Fluor-750

Catalog #1: bsm-33345m-apc-100ul

Applications: IF(IHC-P)

Reactivity: Mouse, Rat

Conjugate: APC

Catalog #1: bsm-33345m-apc-Cy5.5-100ul

Applications: IF(IHC-P)

Reactivity: Mouse, Rat

Conjugate: APC-Cy5.5

Catalog #1: bsm-33345m-apc-cy7-100ul

Applications: IF(IHC-P)

Reactivity: Mouse, Rat

Conjugate: APC-Cy7

Catalog #1: bsm-33345m-biotin-100ul

Applications: IHC-P, IHC-F

Reactivity: Mouse, Rat

Conjugate: Biotin

Catalog #1: bsm-33345m-cy3-100ul

Applications: IF(IHC-P)

Reactivity: Mouse, Rat

Conjugate: Cy3

Catalog #1: bsm-33345m-cy5-100ul

Applications: IF(IHC-P)

Reactivity: Mouse, Rat

Conjugate: Cy5

Catalog #1: bsm-33345m-Cy5.5-100ul

Applications: IF(IHC-P)

Reactivity: Mouse, Rat

Conjugate: Cy5.5

Catalog #1: bsm-33345m-cy7-100ul

Applications: IF(IHC-P)

Reactivity: Mouse, Rat

Conjugate: Cy7

Catalog #1: bsm-33345m-fitc-100ul

Applications: IF(IHC-P)

Reactivity: Mouse, Rat

Conjugate: FITC

Catalog #1: bsm-33345m-hrp-100ul

Applications: IHC-P, IHC-F

Reactivity: Mouse, Rat

Conjugate: HRP

Catalog #1: bsm-33345m-pe-100ul

Applications: IF(IHC-P)

Reactivity: Mouse, Rat

Conjugate: PE

Catalog #1: bsm-33345m-percp-100ul

Applications: IF(IHC-P)

Reactivity: Mouse, Rat

Conjugate: PerCP

Catalog #1: bsm-33345m-percp-Cy5.5-100ul

Applications: IF(IHC-P)

Reactivity: Mouse, Rat

Conjugate: PerCP-Cy5.5

Catalog #1: bsm-33345m-percp-cy7-100ul

Applications: IF(IHC-P)

Reactivity: Mouse, Rat

Conjugate: PerCP-Cy7

Catalog #1: bsm-33345m-rbitc-100ul

Applications: IF(IHC-P)

Reactivity: Mouse, Rat

Conjugate: RBITC

Lane 1: 293; Lane 2: K562 whole cell lysate at 20 \u00b5g per lane, probed with bsm-51058M TGFB2 (220ct16.4.3.1) Monoclonal Antibody at 1:1000 dilution and 4\u2103 overnight incubation, followed by secondary antibody incubation for 60min at room temperature.
Paraformaldehyde-fixed, paraffin embedded human colon carcinoma tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 15 minutes; Blocking buffer (3% BSA) at room temperature for 30min; Antibody incubation with TGFB2 (220ct16.4.3.1) Monoclonal Antibody (bsm-51058M) at 1:50 for 1 hour at 37\u00b0C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded human breast carcinoma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 15 minutes; Blocking buffer (3% BSA) at room temperature for 30min; Antibody incubation with TGFB2 (220ct16.4.3.1) Monoclonal Antibody (bsm-51058M) at 1:50 for 1 hour at 37\u00b0C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.

Catalog #1: bsm-51058M

Applications: WB, IHC-P, IF

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51056M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51041M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-70288M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-70285M

Applications: WB

Reactivity: Human, Rat

Conjugate: Unconjugated

Catalog #1: bsm-70287M

Applications: WB

Reactivity: Human, Rat

Conjugate: Unconjugated

Lane 1: SW480 Cell lysates; probed with TIMP2 (4C7) Monoclonal Antibody, unconjugated (bsm-51320M) at 1:500 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized A549 cells labeled with TIMP2 (4C7) Monoclonal Antibody (bsm-51320M) at 1\/25 dilution followed by secondary antibody (green), cytoplasmic actin staining (red), and nuclear counter stain with DAPI (blue).
K562 cells probed with using TIMP2 (4C7) Monoclonal Antibody bsm-51320M at 1:25 for 30 minutes followed by A488 secondary antibody (green) at 1:200, compared to an isotype control of mouse IgG1 (blue).
Paraformaldehyde-fixed, paraffin embedded Human kidney tissue; Blocked with 3% BSA for 30min at RT; Antibody incubation with TIMP2 (4C7) Monoclonal Antibody, Unconjugated (bsm-51320M ) at 1:25 for 1hr at 37\u00b0C, followed by a conjugated secondary antibody and DAB staining.

Catalog #1: bsm-51320M

Applications: WB, FCM, IHC-P, IF(ICC), IF

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51112M

Applications: WB, ELISA

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-60911m-100ul

Applications: WB

Reactivity: Mouse

Conjugate: Unconjugated

Catalog #1: bsm-33207m-100ul

Applications: WB

Reactivity: Rat

Conjugate: Unconjugated

Catalog #1: bsm-33207M-BF350

Applications: WB

Reactivity: Rat

Conjugate: AbBy Fluor-350

Catalog #1: bsm-33207M-BF405

Applications: WB

Reactivity: Rat

Conjugate: AbBy Fluor-405

Catalog #1: bsm-33207M-BF488

Applications: WB

Reactivity: Rat

Conjugate: AbBy Fluor-488

Catalog #1: bsm-33207M-BF555

Applications: WB

Reactivity: Rat

Conjugate: AbBy Fluor-555

Catalog #1: bsm-33207M-BF594

Applications: WB

Reactivity: Rat

Conjugate: AbBy Fluor-594