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Catalog #1: bsm-52171r-bf594-100l

Applications: IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-594

Catalog #1: bsm-52171r-bf647-100l

Applications: IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-647

Catalog #1: bsm-52171r-bf680-100l

Applications: IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-680

Catalog #1: bsm-52171r-bf750-100l

Applications: IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-750

Catalog #1: bsm-52171r-apc-100l

Applications: IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: APC

Catalog #1: bsm-52171r-apc-Cy5.5-100ul

Applications: IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: APC-Cy5.5

Catalog #1: bsm-52171r-apc-cy7

Applications: IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: APC-Cy7

Catalog #1: bsm-52171r-biotin-100l

Applications: IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: Biotin

Catalog #1: bsm-52171r-cy3-100l

Applications: IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Cy3

Catalog #1: bsm-52171r-cy5-100l

Applications: IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Cy5

Catalog #1: bsm-52171r-Cy5.5-100ul

Applications: IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Cy5.5

Catalog #1: bsm-52171r-cy7-100l

Applications: IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Cy7

Catalog #1: bsm-52171r-fitc-100l

Applications: IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: FITC

Catalog #1: bsm-52171r-hrp-100l

Applications: IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: HRP

Catalog #1: bsm-52171r-pe-100l

Applications: IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: PE

Catalog #1: bsm-52171r-percp-100l

Applications: IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: PerCP

Catalog #1: bsm-52171r-percp-Cy5.5-100ul

Applications: IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: PerCP-Cy5.5

Catalog #1: bsm-52171r-percp-cy7

Applications: IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: PerCP-Cy7

Catalog #1: bsm-52171r-rbitc-100l

Applications: IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: RBITC

HL-60 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20\u2103, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with JAK2(Tyr221) Polyclonal Antibody(bs-3206R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (phospho-JAK2 (Tyr221)) polyclonal Antibody, Unconjugated (bs-3206R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

Catalog #1: bs-3206R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Zebrafish

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Human tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Phospho-JAK2(Y1007+Y1008) Monoclonal Antibody, Unconjugated (bsm-54504R) at 1:200 for 30 minutes at room temperature, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human lung cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Phospho-JAK2(Y1007+Y1008) Monoclonal Antibody, Unconjugated (bsm-54504R) at 1:200 for 30 minutes at room temperature, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Phospho-JAK2(Y1007+Y1008) Monoclonal Antibody, Unconjugated (bsm-54504R) at 1:200 for 30 minutes at room temperature, DAB staining.
Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37\u00b0C for 20 min; Antibody incubation with (Phospho-JAK2(Y1007+Y1008)) Monoclonal Antibody, Unconjugated (bsm-54504R) 1:50, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

Catalog #1: bsm-54504R

Applications: WB, IHC-P, IF(ICC), IP, IHC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-20168R

Applications: WB

Reactivity: Mouse

Conjugate: Unconjugated

Catalog #1: bs-20168R-BF350

Applications: WB

Reactivity: Mouse

Conjugate: AbBy Fluor-350

Catalog #1: bs-20168R-BF405

Applications: WB

Reactivity: Mouse

Conjugate: AbBy Fluor-405

Catalog #1: bs-20168R-BF488

Applications: WB

Reactivity: Mouse

Conjugate: AbBy Fluor-488

Catalog #1: bs-20168R-BF555

Applications: WB

Reactivity: Mouse

Conjugate: AbBy Fluor-555

Catalog #1: bs-20168R-BF594

Applications: WB

Reactivity: Mouse

Conjugate: AbBy Fluor-594

Catalog #1: bs-20168R-BF647

Applications: WB

Reactivity: Mouse

Conjugate: AbBy Fluor-647

Catalog #1: bs-20168R-BF680

Applications: WB

Reactivity: Mouse

Conjugate: AbBy Fluor-680

Catalog #1: bs-20168R-BF750

Applications: WB

Reactivity: Mouse

Conjugate: AbBy Fluor-750

Catalog #1: bs-20168R-APC

Applications: WB

Reactivity: Mouse

Conjugate: APC

Catalog #1: bs-20168R-APC-Cy5.5

Applications: WB

Reactivity: Mouse

Conjugate: APC-Cy5.5

Catalog #1: bs-20168R-APC-Cy7

Applications: WB

Reactivity: Mouse

Conjugate: APC-Cy7

Catalog #1: bs-20168R-Biotin

Applications: WB

Reactivity: Mouse

Conjugate: Biotin

Catalog #1: bs-20168R-Cy3

Applications: WB

Reactivity: Mouse

Conjugate: Cy3

Catalog #1: bs-20168R-Cy5

Applications: WB

Reactivity: Mouse

Conjugate: Cy5

Catalog #1: bs-20168R-Cy5.5

Applications: WB

Reactivity: Mouse

Conjugate: Cy5.5

Catalog #1: bs-20168R-Cy7

Applications: WB

Reactivity: Mouse

Conjugate: Cy7

Catalog #1: bs-20168R-FITC

Applications: WB

Reactivity: Mouse

Conjugate: FITC

Catalog #1: bs-20168R-HRP

Applications: WB

Reactivity: Mouse

Conjugate: HRP

Catalog #1: bs-20168R-PE

Applications: WB

Reactivity: Mouse

Conjugate: PE

Catalog #1: bs-20168R-PE-Cy5

Applications: WB

Reactivity: Mouse

Conjugate: PE-Cy5

Catalog #1: bs-20168R-PE-Cy5.5

Applications: WB

Reactivity: Mouse

Conjugate: PE-Cy5.5

Catalog #1: bs-20168R-PE-Cy7

Applications: WB

Reactivity: Mouse

Conjugate: PE-Cy7

Catalog #1: bs-20168R-PerCP

Applications: WB

Reactivity: Mouse

Conjugate: PerCP

Catalog #1: bs-20168R-PerCP-Cy5.5

Applications: WB

Reactivity: Mouse

Conjugate: PerCP-Cy5.5

Catalog #1: bs-20168R-RBITC

Applications: WB

Reactivity: Mouse

Conjugate: RBITC

Catalog #1: bs-3208r

Applications: ELISA

Reactivity:

Conjugate: Unconjugated