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Catalog #1: bs-70500r

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-70090r

Applications: WB, IHC

Reactivity: Human, Mouse, Rat, Chicken

Conjugate: Unconjugated

Catalog #1: bs-13188r-percp-cy7-100ul

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: PerCP-Cy7

25 ug total protein per lane of various lysates (see on figure) probed with Phospho-FoxO3a (Ser253) monoclonal antibody, unconjugated (bsm-52156R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Phospho-FoxO3a (Ser253) Monoclonal Antibody, Unconjugated (bsm-52156R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Phospho-FoxO3a (Ser253) Monoclonal Antibody, Unconjugated (bsm-52156R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Phospho-FoxO3a (Ser253) Monoclonal Antibody, Unconjugated (bsm-52156R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

Catalog #1: bsm-52156r-100l

Applications: WB, IHC-F, IF, IHC-P

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-52156r-bf350-100ul

Applications: WB, IF

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-350

Catalog #1: bsm-52156r-bf405-100ul

Applications: WB, IF

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-405

Catalog #1: bsm-52156r-bf488-100ul

Applications: WB, IF

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-488

Catalog #1: bsm-52156r-bf555-100ul

Applications: WB, IF

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-555

Catalog #1: bsm-52156r-bf594-100ul

Applications: WB, IF

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-594

Catalog #1: bsm-52156r-bf647-100ul

Applications: WB, IF

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-647

Catalog #1: bsm-52156r-bf680-100ul

Applications: WB, IF

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-680

Catalog #1: bsm-52156r-bf750-100ul

Applications: WB, IF

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-750

Catalog #1: bsm-52156r-apc-100ul

Applications: WB, IF

Reactivity: Human, Mouse, Rat

Conjugate: APC

Catalog #1: bsm-52156r-apc-Cy5.5-100ul

Applications: WB, IF

Reactivity: Human, Mouse, Rat

Conjugate: APC-Cy5.5

Catalog #1: bsm-52156r-apc-cy7-100ul

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: APC-Cy7

Catalog #1: bsm-52156r-biotin-100ul

Applications: WB, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: Biotin

Catalog #1: bsm-52156r-cy3-100ul

Applications: WB, IF

Reactivity: Human, Mouse, Rat

Conjugate: Cy3

Catalog #1: bsm-52156r-cy5-100ul

Applications: WB, IF

Reactivity: Human, Mouse, Rat

Conjugate: Cy5

Catalog #1: bsm-52156r-Cy5.5-100ul

Applications: WB, IF

Reactivity: Human, Mouse, Rat

Conjugate: Cy5.5

Catalog #1: bsm-52156r-cy7-100ul

Applications: WB, IF

Reactivity: Human, Mouse, Rat

Conjugate: Cy7

Catalog #1: bsm-52156r-fitc-100ul

Applications: WB, IF

Reactivity: Human, Mouse, Rat

Conjugate: FITC

Catalog #1: bsm-52156r-hrp-100ul

Applications: WB, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: HRP

Catalog #1: bsm-52156r-pe-100ul

Applications: WB, IF

Reactivity: Human, Mouse, Rat

Conjugate: PE

Catalog #1: bsm-52156r-pe-cy5-100ul

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: PE-Cy5

Catalog #1: bsm-52156r-pe-Cy5.5-100ul

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: PE-Cy5.5

Catalog #1: bsm-52156r-pe-cy7-100ul

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: PE-Cy7

Catalog #1: bsm-52156r-percp-100ul

Applications: WB, IF

Reactivity: Human, Mouse, Rat

Conjugate: PerCP

Catalog #1: bsm-52156r-percp-Cy5.5-100ul

Applications: WB, IF

Reactivity: Human, Mouse, Rat

Conjugate: PerCP-Cy5.5

Catalog #1: bsm-52156r-percp-cy7-100ul

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: PerCP-Cy7

Catalog #1: bsm-52156r-rbitc-100ul

Applications: WB, IF

Reactivity: Human, Mouse, Rat

Conjugate: RBITC

Catalog #1: bs-70110r

Applications: WB

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-70106r

Applications: WB

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-70111r

Applications: WB, IHC

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-70112r

Applications: WB

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-70116r

Applications: WB

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-70506r

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-70507r

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-70508r

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-70121r

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-70122r

Applications: WB, IHC

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-70125r

Applications: WB

Reactivity: Rat

Conjugate: Unconjugated

Catalog #1: bs-70128r

Applications: WB

Reactivity: Mouse, Rat

Conjugate: Unconjugated

ChIP assays were performed using HeLa cells treated with colcemid, the H3K27me3S28p antibody (bs-53053R) and optimized PCR primer pairs for qPCR. ChIP was performed using sheared chromatin from 10,000 cells. A titration of the antibody consisting of 1, 5 and 10 \u03bcl per ChIP experiment was analysed. Additionally, ChIP was performed after incubation of the antibody with 5 nmol blocking peptide (cat. No. sp-091-050) for 1 hour at room temperature. IgG (5 \u03bcg\/IP) was used as negative IP control. QPCR was performed with primers for the promoters of the active genes c-fos and RPL30 and for the inactive gene MYOD. The figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).
To determine the titer, an ELISA was performed using a serial dilution of the H3K27me3S28p antibody (bs-53053R). The antigen used was a peptide containing the histone modifications of interest. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:8,300.
A Dot Blot analysis was performed to test the cross reactivity of the H3K27me3S28p antibody (bs-53053R) with peptides containing other modifications of histone H3 and H4 and unmodified sequences from histone H3. One hundred to 0.2 pmol of the peptides were spotted on a membrane. The antibody was used at a dilution of 1:20,000. The figure shows a high specificity of the antibody for the peptide containing the modifications of interest.
Hela cells were stained with the H3K27me3S28p antibody (bs-53053R) and with DAPI. Cells were fixed with formaldehyde, permeabilized with sodium citrate and Triton X100 and blocked with PBS containing 2.5% BSA. Left side: cells were immunofluorescently labelled with the H3K27me3S28p antibody (diluted 1:200 and incubated for 1 hour at room temperature) followed by goat anti-rabbit antibody conjugated to DyLight 488. Right side: the nuclei were stained with DAPI, which specifically labels DNA. Phosphorylation of H3 on serine 28 is increased during late G2 phase and reaches a maximum in metaphase cells. This may explain the different staining intensities of different cells.

Catalog #1: bs-53053R

Applications: WB, ELISA, IP, IF, dot-blot, ChIP-seq

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-70130r

Applications: WB

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-10325r-percp-cy7

Applications: WB, IF(IHC-P)

Reactivity: Human

Conjugate: PerCP-Cy7

Paraformaldehyde-fixed, paraffin embedded Human thyroid; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with HER3\/ErbB3 Phospho (pY1222) Monoclonal Antibody, Unconjugated (bsm-54686R) at 1:100 for 30 minutes at room temperature, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human skin; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with HER3\/ErbB3 Phospho (pY1222) Monoclonal Antibody, Unconjugated (bsm-54686R) at 1:100 for 30 minutes at room temperature, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human placenta; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with HER3\/ErbB3 Phospho (pY1222) Monoclonal Antibody, Unconjugated (bsm-54686R) at 1:100 for 30 minutes at room temperature, DAB staining.

Catalog #1: bsm-54686R

Applications: WB, IHC-P

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-70516r

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-70517r

Applications: WB

Reactivity: Human, Mouse, Rat, Chicken, Xenopus

Conjugate: Unconjugated