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Catalog #1: bs-15534R-APC-Cy5.5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: APC-Cy5.5

Catalog #1: bs-15534R-APC-Cy7

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: APC-Cy7

Catalog #1: bs-15534R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human

Conjugate: Biotin

Catalog #1: bs-15534R-Cy3

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Cy3

Catalog #1: bs-15534R-Cy5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Cy5

Catalog #1: bs-15534R-Cy5.5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Cy5.5

Catalog #1: bs-15534R-Cy7

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Cy7

Catalog #1: bs-15534R-FITC

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: FITC

Catalog #1: bs-15534R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human

Conjugate: HRP

Catalog #1: bs-15534R-PE

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: PE

Catalog #1: bs-15534R-PE-Cy5

Applications: WB, FCM

Reactivity: Human

Conjugate: PE-Cy5

Catalog #1: bs-15534R-PE-Cy5.5

Applications: WB, FCM

Reactivity: Human

Conjugate: PE-Cy5.5

Catalog #1: bs-15534R-PE-Cy7

Applications: WB, FCM

Reactivity: Human

Conjugate: PE-Cy7

Catalog #1: bs-15534R-PerCP

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: PerCP

Catalog #1: bs-15534R-PerCP-Cy5.5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: PerCP-Cy5.5

Catalog #1: bs-15534R-RBITC

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: RBITC

25 ug total protein per lane of various lysates (see on figure) probed with Bcl-2 monoclonal antibody, unconjugated (bsm-10846M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
The Hela (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.).Primary Antibody (green):Mouse Anti-Bcl-2 antibody (bsm-10846M): 1 \u03bcg\/10^6 cells; Secondary Antibody (white blue): Goat anti-Mouse IgG-BF488(bs-60296G-BF488): 1 \u03bcg\/test. Blank control (black): PBS. Acquisition of 20,000 events was performed.
4% Paraformaldehyde-fixed Hela (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (Bcl-2) monoclonal Antibody, unconjugated (bsm-10846M) 1:100, 90 min at 37\u00b0C; followed by conjugated Goat Anti-Mouse IgG antibody (green, bs-60296G-BF488) at 37\u00b0C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.

Catalog #1: bsm-10846m

Applications: WB, ICC/IF

Reactivity: Human

Conjugate: Unconjugated

Lane 1: LOVO lysates; Lane 2: MCF-7 lysates probed with Bcl-2 Polyclonal Antibody, Unconjugated (bs-33411M) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded Rat lymph node; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Bcl-2 Monoclonal Antibody, Unconjugated (bsm-33411M) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human pancreatic cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Bcl-2 Monoclonal Antibody, Unconjugated (bsm-33411M) at 1:200 overnight at 4\u00b0C, DAB staining.
Tissue\/cell: Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (Bcl-2) monoclonal Antibody, Unconjugated (bs-33411M) 1:100, 90 minutes at 37\u00b0C; followed by a CY3 conjugated Goat Anti-Mouse IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

Catalog #1: bsm-33411M

Applications: WB, IHC-P, IHC-F, IF, ICC/IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1: LOVO lysates; Lane 2: MCF7 lysates probed with Bcl-2 Polyclonal Antibody, Unconjugated (bs-33047M) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
Lane 1: Jurkat cell lysates; Lane 2: Hela cell lysates; Lane 3: HL60 cell lysates probed with Bcl-2 Monoclonal Antibody, Unconjugated (bsm-33047M) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded Human colon cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with \r\nBcl2 Monoclonal Antibody, Unconjugated (bsm-33047M) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with \r\nBcl2 Monoclonal Antibody, Unconjugated (bsm-33047M) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bsm-33047M

Applications: WB, IHC-P, IHC-F, IF, ICC/IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-33412M

Applications: WB, Flow-Cyt

Reactivity: Human, Mouse, Rat, Bovine, Dog, Pig, Sheep, Rabbit, Horse, Guinea Pig

Conjugate: Unconjugated

Ctll-2 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20\u2103, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Bcl-2 monoclonal Antibody(bsm-33413M)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Ctll-2 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20\u2103, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Bcl-2 monoclonal Antibody(bsm-33413M)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

Catalog #1: bsm-33413M

Applications: WB, Flow-Cyt

Reactivity: Human, Mouse, Rat, Bovine, Dog, Pig, Sheep, Rabbit, Horse, Guinea Pig

Conjugate: Unconjugated

Catalog #1: bsm-10846m-bf350-100ul

Applications: WB

Reactivity: Human

Conjugate: AbBy Fluor-350

Catalog #1: bsm-33047M-BF350

Applications: WB, IF

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-350

Catalog #1: bsm-33411M-BF350

Applications: WB, IF

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-350

Catalog #1: bsm-33412M-BF350

Applications: WB

Reactivity: Human, Mouse, Rat, Bovine, Dog, Pig, Sheep, Rabbit, Horse, Guinea Pig

Conjugate: AbBy Fluor-350

Catalog #1: bsm-33413M-BF350

Applications: WB

Reactivity: Human, Mouse, Rat, Bovine, Dog, Pig, Sheep, Rabbit, Horse, Guinea Pig

Conjugate: AbBy Fluor-350

Catalog #1: bsm-10846m-bf405-100ul

Applications: WB

Reactivity: Human

Conjugate: AbBy Fluor-405

Catalog #1: bsm-33047M-BF405

Applications: WB, IF

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-405

Catalog #1: bsm-33411M-BF405

Applications: WB, IF

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-405

Catalog #1: bsm-33412M-BF405

Applications: WB

Reactivity: Human, Mouse, Rat, Bovine, Dog, Pig, Sheep, Rabbit, Horse, Guinea Pig

Conjugate: AbBy Fluor-405

Catalog #1: bsm-33413M-BF405

Applications: WB

Reactivity: Human, Mouse, Rat, Bovine, Dog, Pig, Sheep, Rabbit, Horse, Guinea Pig

Conjugate: AbBy Fluor-405

Catalog #1: bsm-10846m-bf488-100ul

Applications: WB

Reactivity: Human

Conjugate: AbBy Fluor-488

Catalog #1: bsm-33047M-BF488

Applications: WB, IF

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-488

Catalog #1: bsm-33411M-BF488

Applications: WB, IF

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-488

Catalog #1: bsm-33412M-BF488

Applications: WB

Reactivity: Human, Mouse, Rat, Bovine, Dog, Pig, Sheep, Rabbit, Horse, Guinea Pig

Conjugate: AbBy Fluor-488

Catalog #1: bsm-33413M-BF488

Applications: WB

Reactivity: Human, Mouse, Rat, Bovine, Dog, Pig, Sheep, Rabbit, Horse, Guinea Pig

Conjugate: AbBy Fluor-488

Catalog #1: bsm-10846m-bf555-100ul

Applications: WB

Reactivity: Human

Conjugate: AbBy Fluor-555

Catalog #1: bsm-33047M-BF555

Applications: WB, IF

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-555

Catalog #1: bsm-33411M-BF555

Applications: WB, IF

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-555

Catalog #1: bsm-33412M-BF555

Applications: WB

Reactivity: Human, Mouse, Rat, Bovine, Dog, Pig, Sheep, Rabbit, Horse, Guinea Pig

Conjugate: AbBy Fluor-555

Catalog #1: bsm-33413M-BF555

Applications: WB

Reactivity: Human, Mouse, Rat, Bovine, Dog, Pig, Sheep, Rabbit, Horse, Guinea Pig

Conjugate: AbBy Fluor-555

Catalog #1: bsm-10846m-bf594-100ul

Applications: WB

Reactivity: Human

Conjugate: AbBy Fluor-594

Catalog #1: bsm-33047M-BF594

Applications: WB, IF

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-594

Catalog #1: bsm-33411M-BF594

Applications: WB, IF

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-594

Catalog #1: bsm-33412M-BF594

Applications: WB

Reactivity: Human, Mouse, Rat, Bovine, Dog, Pig, Sheep, Rabbit, Horse, Guinea Pig

Conjugate: AbBy Fluor-594

Catalog #1: bsm-33413M-BF594

Applications: WB

Reactivity: Human, Mouse, Rat, Bovine, Dog, Pig, Sheep, Rabbit, Horse, Guinea Pig

Conjugate: AbBy Fluor-594

Catalog #1: bsm-10846m-bf647-100ul

Applications: WB

Reactivity: Human

Conjugate: AbBy Fluor-647

Catalog #1: bsm-33047M-BF647

Applications: WB, IF

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-647