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Catalog #1: bs-10176R-Cy5.5

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Cy5.5

Catalog #1: bs-10176R-Cy7

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Cy7

Catalog #1: bs-10176R-FITC

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: FITC

Catalog #1: bs-10176R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human

Conjugate: HRP

Catalog #1: bs-10176R-PE

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: PE

Catalog #1: bs-10176R-PE-Cy5

Applications: WB

Reactivity: Human

Conjugate: PE-Cy5

Catalog #1: bs-10176R-PE-Cy5.5

Applications: WB

Reactivity: Human

Conjugate: PE-Cy5.5

Catalog #1: bs-10176R-PE-Cy7

Applications: WB

Reactivity: Human

Conjugate: PE-Cy7

Catalog #1: bs-10176R-PerCP

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: PerCP

Catalog #1: bs-10176R-PerCP-Cy5.5

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: PerCP-Cy5.5

Catalog #1: bs-10176R-RBITC

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: RBITC

The Hela (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.).Primary Antibody (green):Rabbit Anti-AKT1S1 antibody (bsm-61149R,1:100); Secondary Antibody (white blue): Goat anti-Rabbit IgG-BF488 (bs-60295G-BF488): 1 \u03bcg\/test. Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.
4% Paraformaldehyde-fixed Hela (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (AKT1S1) monoclonal Antibody, unconjugated (bsm-61149R) 1:100, 90 min at 37\u00b0C; followed by conjugated Goat Anti-Rabbit IgG antibody (green, bs-60295G-BF488) at 37\u00b0C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.

Catalog #1: bsm-61149r

Applications: WB, IHC-P, IHC-F, IP, IF, Flow-Cyt, ICC/IF

Reactivity: Human, Mouse

Conjugate: Unconjugated

25 ug total protein per lane of various lysates (see on figure) probed with AKT1S1 monoclonal antibody, unconjugated (bsm-61150R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
25 ug total protein per lane of various lysates (see on figure) probed with AKT1S1 monoclonal antibody, unconjugated (bsm-61150R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Catalog #1: bsm-61150r-TR

Applications: WB, IP

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-61149r-bf350-100ul

Applications: WB, IF, Flow-Cyt

Reactivity: Human, Mouse

Conjugate: AbBy Fluor-350

Catalog #1: bsm-61150r-bf350-100ul

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-350

Catalog #1: bsm-61149r-bf405-100ul

Applications: WB, IF, Flow-Cyt

Reactivity: Human, Mouse

Conjugate: AbBy Fluor-405

Catalog #1: bsm-61150r-bf405-100ul

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-405

Catalog #1: bsm-61149r-bf488-100ul

Applications: WB, IF, Flow-Cyt

Reactivity: Human, Mouse

Conjugate: AbBy Fluor-488

Catalog #1: bsm-61150r-bf488-100ul

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-488

Catalog #1: bsm-61149r-bf555-100ul

Applications: WB, IF, Flow-Cyt

Reactivity: Human, Mouse

Conjugate: AbBy Fluor-555

Catalog #1: bsm-61150r-bf555-100ul

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-555

Catalog #1: bsm-61149r-bf594-100ul

Applications: WB, IF, Flow-Cyt

Reactivity: Human, Mouse

Conjugate: AbBy Fluor-594

Catalog #1: bsm-61150r-bf594-100ul

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-594

Catalog #1: bsm-61149r-bf647-100ul

Applications: WB, IF

Reactivity: Human, Mouse

Conjugate: AbBy Fluor-647

Catalog #1: bsm-61150r-bf647-100ul

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-647

Catalog #1: bsm-61149r-bf680-100ul

Applications: WB, IF

Reactivity: Human, Mouse

Conjugate: AbBy Fluor-680

Catalog #1: bsm-61150r-bf680-100ul

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-680

Catalog #1: bsm-61149r-bf750-100ul

Applications: WB, IF, Flow-Cyt

Reactivity: Human, Mouse

Conjugate: AbBy Fluor-750

Catalog #1: bsm-61150r-bf750-100ul

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-750

Catalog #1: bsm-61149r-apc-100ul

Applications: WB, IF, Flow-Cyt

Reactivity: Human, Mouse

Conjugate: APC

Catalog #1: bsm-61150r-apc-100ul

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: APC

Catalog #1: bsm-61149r-apc-Cy5.5-100ul

Applications: WB, IF, Flow-Cyt

Reactivity: Human, Mouse

Conjugate: APC-Cy5.5

Catalog #1: bsm-61150r-apc-Cy5.5-100ul

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: APC-Cy5.5

Catalog #1: bsm-61149r-apc-cy7-100ul

Applications: WB, Flow-Cyt

Reactivity: Human, Mouse

Conjugate: APC-Cy7

Catalog #1: bsm-61150r-apc-cy7-100ul

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: APC-Cy7

Catalog #1: bsm-61149r-biotin-100ul

Applications: WB, IHC-P, IHC-F, IP

Reactivity: Human, Mouse

Conjugate: Biotin

Catalog #1: bsm-61150r-biotin-100ul

Applications: WB, IP

Reactivity: Human, Mouse, Rat

Conjugate: Biotin

Catalog #1: bsm-61149r-cy3-100ul

Applications: WB, IF, Flow-Cyt

Reactivity: Human, Mouse

Conjugate: Cy3

Catalog #1: bsm-61150r-cy3-100ul

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: Cy3

Catalog #1: bsm-61149r-cy5-100ul

Applications: WB, IF, Flow-Cyt

Reactivity: Human, Mouse

Conjugate: Cy5

Catalog #1: bsm-61150r-cy5-100ul

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: Cy5

Catalog #1: bsm-61149r-Cy5.5-100ul

Applications: WB, IF, Flow-Cyt

Reactivity: Human, Mouse

Conjugate: Cy5.5

Catalog #1: bsm-61150r-Cy5.5-100ul

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: Cy5.5

Catalog #1: bsm-61149r-cy7-100ul

Applications: WB, IF, Flow-Cyt

Reactivity: Human, Mouse

Conjugate: Cy7

Catalog #1: bsm-61150r-cy7-100ul

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: Cy7

Catalog #1: bsm-61149r-fitc-100ul

Applications: WB, IF, Flow-Cyt

Reactivity: Human, Mouse

Conjugate: FITC

Catalog #1: bsm-61150r-fitc-100ul

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: FITC

Catalog #1: bsm-61149r-hrp-100ul

Applications: WB, IHC-P, IHC-F

Reactivity: Human, Mouse

Conjugate: HRP