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Catalog #1: bs-0912R-Cy5

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Others

Conjugate: Cy5

Catalog #1: bs-2143R-Cy5

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Others

Conjugate: Cy5

Catalog #1: bs-0912R-Cy5.5

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Others

Conjugate: Cy5.5

Catalog #1: bs-2143R-Cy5.5

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Others

Conjugate: Cy5.5

Catalog #1: bs-0912R-Cy7

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Others

Conjugate: Cy7

Catalog #1: bs-2143R-Cy7

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Others

Conjugate: Cy7

Catalog #1: bs-0912R-FITC

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Others

Conjugate: FITC

Catalog #1: bs-2143R-FITC

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Others

Conjugate: FITC

Catalog #1: bs-0912R-HRP

Applications: ELISA, IHC-P, IHC-F

Reactivity: Others

Conjugate: HRP

Catalog #1: bs-2143R-HRP

Applications: ELISA, IHC-P, IHC-F

Reactivity: Others

Conjugate: HRP

Catalog #1: bs-0912R-PerCP

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Others

Conjugate: PerCP

Catalog #1: bs-2143R-PerCP

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Others

Conjugate: PerCP

Catalog #1: bs-0912R-PerCP-Cy5.5

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Others

Conjugate: PerCP-Cy5.5

Catalog #1: bs-2143R-PerCP-Cy5.5

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Others

Conjugate: PerCP-Cy5.5

Catalog #1: bs-0912R-RBITC

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Others

Conjugate: RBITC

Catalog #1: bs-2143R-RBITC

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Others

Conjugate: RBITC

Paraformaldehyde-fixed, paraffin embedded Human brain glioma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Bax Monoclonal Antibody, Unconjugated (bsm-33279M) at 1:300 overnight at 4\u00b0C, followed by Goat Anti-Mouse IgG (H+L) Cy3 conjugated secondary antibody. DAPI staining of the nucleus was done and then detected.
Lane 1: Cerebrum lysates probed with Bax Monoclonal Antibody, Unconjugated (bsm-33279M) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Tissue\/cell: Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (Bax) monoclonal Antibody, Unconjugated (bs-33279M) 1:100, 90 minutes at 37\u00b0C; followed by a CY3 conjugated Goat Anti-Mouse IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
Paraformaldehyde-fixed, paraffin embedded (human gastric carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (Bax ) Monoclonal Antibody, Unconjugated (bsm-33279M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.

Catalog #1: bsm-33279M

Applications: WB, IHC-P, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Unconjugated

Tissue\/cell: Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (Bax) monoclonal Antibody, Unconjugated (bsm-33283M) 1:100, 90 minutes at 37\u00b0C; followed by a CY3 conjugated Goat Anti-Mouse IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
Lane 1: Human 293T cell lysates; Lane 2: Human HT-1080 cell lysates; Lane 3: Human Raji cell lysates; Lane 4: Human SH-SY5Y cell lysates probed with Bax Monoclonal Antibody, Unconjugated (bsm-33283M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bsm-33283m-TR

Applications: WB, ICC/IF

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-33279M-BF350

Applications: WB, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse

Conjugate: AbBy Fluor-350

Catalog #1: bsm-33279M-BF405

Applications: WB, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse

Conjugate: AbBy Fluor-405

Catalog #1: bsm-33279M-BF488

Applications: WB, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse

Conjugate: AbBy Fluor-488

Catalog #1: bsm-33279M-BF555

Applications: WB, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse

Conjugate: AbBy Fluor-555

Catalog #1: bsm-33279M-BF594

Applications: WB, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse

Conjugate: AbBy Fluor-594

Catalog #1: bsm-33279M-BF647

Applications: WB, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse

Conjugate: AbBy Fluor-647

Catalog #1: bsm-33279M-BF680

Applications: WB, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse

Conjugate: AbBy Fluor-680

Catalog #1: bsm-33279M-BF750

Applications: WB, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse

Conjugate: AbBy Fluor-750

Catalog #1: bsm-33279M-APC

Applications: WB, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse

Conjugate: APC

Catalog #1: bsm-33279M-APC-Cy5.5

Applications: WB, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse

Conjugate: APC-Cy5.5

Catalog #1: bsm-33279M-APC-Cy7

Applications: WB, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse

Conjugate: APC-Cy7

Catalog #1: bsm-33279M-Biotin

Applications: WB, IHC-P

Reactivity: Human, Mouse

Conjugate: Biotin

Catalog #1: bsm-33279M-Cy3

Applications: WB, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Cy3

Catalog #1: bsm-33279M-Cy5

Applications: WB, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Cy5

Catalog #1: bsm-33279M-Cy5.5

Applications: WB, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Cy5.5

Catalog #1: bsm-33279M-Cy7

Applications: WB, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Cy7

Catalog #1: bsm-33279M-FITC

Applications: WB, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse

Conjugate: FITC

Catalog #1: bsm-33279M-HRP

Applications: WB, IHC-P

Reactivity: Human, Mouse

Conjugate: HRP

Catalog #1: bsm-33279M-PE

Applications: WB, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse

Conjugate: PE

Catalog #1: bsm-33279M-PE-Cy5

Applications: WB

Reactivity: Human, Mouse

Conjugate: PE-Cy5

Catalog #1: bsm-33279M-PE-Cy5.5

Applications: WB

Reactivity: Human, Mouse

Conjugate: PE-Cy5.5

Catalog #1: bsm-33279M-PE-Cy7

Applications: WB

Reactivity: Human, Mouse

Conjugate: PE-Cy7

Catalog #1: bsm-33279M-PerCP

Applications: WB, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse

Conjugate: PerCP

Catalog #1: bsm-33279M-PerCP-Cy5.5

Applications: WB, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse

Conjugate: PerCP-Cy5.5

Catalog #1: bsm-33279M-RBITC

Applications: WB, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse

Conjugate: RBITC

Formalin-fixed and paraffin embedded rat brain labeled with Anti- Bax Polyclonal Antibody, Unconjugated (bs-0127M) at 1:200, followed by conjugation to the secondary antibody and DAB staining.
Tissue\/cell: Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (Bax) polyclonal Antibody, Unconjugated (bs-0127M) 1:100, 90 minutes at 37\u00b0C; followed by a CY3 conjugated Goat Anti-Mouse IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
Tissue\/cell: Sh-sy5y cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (Bax) polyclonal Antibody, Unconjugated (bs-0127M) 1:100, 90 minutes at 37\u00b0C; followed by a CY3 conjugated Goat Anti-Mouse IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
25 ug total protein per lane of various lysates (see on figure) probed with Bax monoclonal antibody, unconjugated (bs-0127M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Catalog #1: bs-0127m

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded: rat brain tissue labeled with Anti-Bax Polyclonal Antibody, Unconjugated (bs-0127R) at 1:800 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded rabbit brain tissue labeled with Anti-Bax Polyclonal Antibody, Unconjugated (bs-0127R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
L1 rat brain, L2 mouse liver lysates probed (bs-0127R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted band and observed band size: 21kDa.
Paraformaldehyde-fixed, paraffin embedded rat lung; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with Anti \u2013BAX Polyclonal Antibody, Unconjugated (bs-0127R) at 1:200 overnight at 4\u00b0C, followed by a conjugated secondary for 90 minutes and DAB staining

Catalog #1: bs-0127R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), IP

Reactivity: Human, Mouse, Rat, Rabbit

Conjugate: Unconjugated

Catalog #1: bs-0127R-BF350

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Rabbit

Conjugate: AbBy Fluor-350

Catalog #1: bs-0127R-BF405

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Rabbit

Conjugate: AbBy Fluor-405

Catalog #1: bs-0127R-BF488

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Rabbit

Conjugate: AbBy Fluor-488