Caspase 8 Polyclonal Antibody, PE-Cy5.5 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-0052R-PE-Cy5.5-100uL |
| Product Name | Caspase 8 Polyclonal Antibody, PE-Cy5.5 Conjugated |
| Applications |
WB
FCM
|
| Reactivity | Human, Rat |
| Predicted Reactivity | Mouse, Dog, Cow, Pig, Horse |
| Specifications | |
| Conjugation | PE-Cy5.5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from rat CASP8 subunit p10 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 12370 |
| Swiss Prot | Q9JHX4 |
| Subcellular location | Cytoplasm |
| Synonyms | MACH; Mch5; FLICE; CASP-8; Caspase-8; Casp8 |
| Background | Most upstream protease of the activation cascade of caspases responsible for the TNFRSF6/FAS mediated and TNFRSF1A induced cell death. Binding to the adapter molecule FADD recruits it to either receptor. The resulting aggregate called death-inducing signaling complex (DISC) performs CASP8 proteolytic activation. The active dimeric enzyme is then liberated from the DISC and free to activate downstream apoptotic proteases. Proteolytic fragments of the N-terminal propeptide (termed CAP3, CAP5 and CAP6) are likely retained in the DISC. Cleaves and activates CASP3, CASP4, CASP6, CASP7, CASP9 and CASP1. May participate in the GZMB apoptotic pathways. Cleaves ADPRT. Hydrolyzes the small-molecule substrate, Ac-Asp-Glu-Val-Asp-|-AMC. Likely target for the cowpox virus CRMA death inhibitory protein. |
| Application Dilution | |
| WB | 1:300-5000 |
| FCM | 1:20-100 |
Frequently asked questions
Which applications is Caspase 8 Polyclonal Antibody, PE-Cy5.5 Conjugated (BS-0052R-PE-CY5-5) validated for, and at what dilution?
BS-0052R-PE-CY5-5 is validated for WB (1:300-5000); FCM (1:20-100). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Caspase 8 Polyclonal Antibody, PE-Cy5.5 Conjugated (BS-0052R-PE-CY5-5) react with?
Tested reactivity: Human, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Dog, Cow, Pig, Horse.
How should BS-0052R-PE-CY5-5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Caspase 8 Polyclonal Antibody, PE-Cy5.5 Conjugated (BS-0052R-PE-CY5-5)?
KLH conjugated synthetic peptide derived from rat CASP8 subunit p10
What are the excitation and emission wavelengths of the PE-Cy5.5 conjugate (BS-0052R-PE-CY5-5)?
PE-Cy5.5 on BS-0052R-PE-CY5-5: excitation / emission 488nm/694nm.