14-3-3 Alpha + Beta + Gamma + Delta + Epsilon Antibody
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-0237r-100uL |
| Product Name | 14-3-3 Alpha + Beta + Gamma + Delta + Epsilon Antibody |
| Applications |
WB
FCM
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Sheep, Fruit Fly, yeast |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human 14-3-3 |
| Immunogen Range | 174-245/245 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. |
| Target | |
| Gene ID | 7529 |
| Swiss Prot | P31946 |
| Subcellular location | Cytoplasm |
| Synonyms | HS1; GW128; YWHAA; KCIP-1; HEL-S-1; 14-3-3 protein beta/alpha; Protein 154; Protein kinase C inhibitor protein 1; YWHAB |
| Background | Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Negative regulator of osteogenesis. Blocks the nuclear translocation of the phosphorylated form (by AKT1) of SRPK2 and antagonizes its stimulatory effect on cyclin D1 expression resulting in blockage of neuronal apoptosis elicited by SRPK2. |
| Application Dilution | |
| WB | 1:300-5000 |
| FCM | 1:20-100 |
| IHC-P | 1:200-400 |
| IHC-F | 1:100-500 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is 14-3-3 Alpha + Beta + Gamma + Delta + Epsilon Antibody (bs-0237R) validated for, and at what dilution?
bs-0237R is validated for WB (1:300-5000); FCM (1:20-100); IHC-P (1:200-400); IHC-F (1:100-500); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does 14-3-3 Alpha + Beta + Gamma + Delta + Epsilon Antibody (bs-0237R) react with?
Tested reactivity: Human, Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Sheep, Fruit Fly, yeast.
How should bs-0237R be stored?
Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise 14-3-3 Alpha + Beta + Gamma + Delta + Epsilon Antibody (bs-0237R)?
KLH conjugated synthetic peptide derived from human 14-3-3 Immunogen range: 174-245/245.