Rabbit Anti-Rat IgG Antibody (H+L), Biotin Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-0293R-Biotin |
| Product Name | Rabbit Anti-Rat IgG Antibody (H+L), Biotin Conjugated |
| Applications |
WB
IHC-P
IHC-F
|
| Reactivity | Rat |
| Specifications | |
| Conjugation | Biotin |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C for 12 months. |
| Target | |
| Background | Immunoglobulin G (IgG), is one of the most abundant proteins in serum with normal levels between 8-17 mg/mL in adult blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants. |
| Application Dilution | |
| WB | 1:300-5000 |
| IHC-P | 1:200-400 |
| IHC-F | 1:100-500 |
Frequently asked questions
Which applications is Rabbit Anti-Rat IgG Antibody (H+L), Biotin Conjugated (bs-0293R-Biotin) validated for, and at what dilution?
bs-0293R-Biotin is validated for WB (1:300-5000); IHC-P (1:200-400); IHC-F (1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Rabbit Anti-Rat IgG Antibody (H+L), Biotin Conjugated (bs-0293R-Biotin) react with?
Tested reactivity: Rat.
How should bs-0293R-Biotin be stored?
Store at -20°C for 12 months. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.