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Rabbit Anti-Mouse IgM Antibody (H+L), AbBy Fluor® 680 Conjugated

Applications

  • FCM
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Mouse
Overview
Catalog # bs-0368R-BF680
Product Name Rabbit Anti-Mouse IgM Antibody (H+L), AbBy Fluor® 680 Conjugated
Applications FCM, IF(IHC-P), IF(IHC-F), IF(ICC)
Reactivity Mouse
Specifications
Conjugation AbBy Fluor® 680
Host Rabbit
Clonality Polyclonal
Isotype IgM
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage Condition Store at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles
Target
Background Immunoglobulin IgM normally constitutes about 10% of serum immunoglobulins. IgM antibody is prominent in early immune responses to most antigens and is largely confined to plasma due to it's large size. Monomeric IgM is expressed as a membrane bound antibody on the surface of B cells and as a pentamer when secreted by plasma cells.
Application Dilution
FCM 1:20-100
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200