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MCT 8 Polyclonal Antibody

Mouse cells probed with Rabbit Anti-MOT8\/SLC16A2 Polyclonal Antibody (bs-11434R) at 1:100 for 60 minutes followed by incubation with Goat Anti-Rabbit IgG FITC conjugated secondary (bs-0295G-FITC) at 1:100 (green) for 40 minutes compared to control cells (blue).
Mouse cells probed with Rabbit Anti-MOT8\/SLC16A2 Polyclonal Antibody (bs-11434R) at 1:100 for 60 minutes followed by incubation with Goat Anti-Rabbit IgG FITC conjugated secondary (bs-0295G-FITC) at 1:100 (green) for 40 minutes compared to control cells (blue).
Rat H9C2 cells probed with Rabbit Anti-MOT8\/SLC16A2 Polyclonal Antibody, Unconjugated (bs-11434R) at 3ug for 30 minutes followed by incubation with a conjugated secondary (bs-0295G-FITC)(green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).
Rat H9C2 cells probed with Rabbit Anti-MOT8\/SLC16A2 Polyclonal Antibody, Unconjugated (bs-11434R) at 3ug for 30 minutes followed by incubation with a conjugated secondary (bs-0295G-FITC)(green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).
Mouse heart lysates probed with MOT8 Polyclonal Antibody, Unconjugated (bs-11434R) at 1:1000 dilution and 4\u02daC overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37\u02daC.
Mouse heart lysates probed with MOT8 Polyclonal Antibody, Unconjugated (bs-11434R) at 1:1000 dilution and 4\u02daC overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37\u02daC.
U2-OS cells(black) were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with MOT8\/SLC16A2 Polyclonal Antibody(bs-11434R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
U2-OS cells(black) were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with MOT8\/SLC16A2 Polyclonal Antibody(bs-11434R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Lane 1: Human U251 cell lysates; Lane 2: Human U87MG cell lysates; Lane 3: Human SH-SY5Y cell lysates probed with MCT 8 Polyclonal Antibody, Unconjugated (bs-11434R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37\u02daC.
Lane 1: Human U251 cell lysates; Lane 2: Human U87MG cell lysates; Lane 3: Human SH-SY5Y cell lysates probed with MCT 8 Polyclonal Antibody, Unconjugated (bs-11434R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37\u02daC.
Bioss unconjugated antibody vial

Applications

  • WB
  • ELISA

Reactivity

  • Human
  • Mouse
  • Rat

Predicted Reactivity

  • Rabbit
Overview
Catalog # bs-11434R-100uL
Product Name MCT 8 Polyclonal Antibody
Applications
WB ELISA
Reactivity Human, Mouse, Rat
Predicted Reactivity Rabbit
Specifications
Conjugation Unconjugated
Host Rabbit
Source KLH conjugated synthetic peptide derived from human MOT8/SLC16A2
Immunogen Range 101-200/539
Clonality Polyclonal
Isotype IgG
Concentration Lot Dependent
Purification purified by Protein A.
Storage Buffer 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Shipped at 4℃. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID 6567
Swiss Prot P36021
Subcellular location Cell membrane
Synonyms AHDS; MCT7; MCT8; XPCT; MCT 7; MCT 8; MRX22; DXS128; DXS128E; Monocarboxylate transporter 8; Monocarboxylate transporter 7; Solute carrier family 16 member 2; X-linked PEST-containing transporter; SLC16A2
Background Very active and specific thyroid hormone transporter. Stimulates cellular uptake of thyroxine (T4), triiodothyronine (T3), reverse triiodothyronine (rT3) and diidothyronine. Does not transport Leu, Phe, Trp or Tyr (By similarity).
Application Dilution
WB 1:300-5000
ELISA 1:5000-10000

Frequently asked questions

Which applications is MCT 8 Polyclonal Antibody (bs-11434R) validated for, and at what dilution?

bs-11434R is validated for WB (1:300-5000); ELISA (1:5000-10000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does MCT 8 Polyclonal Antibody (bs-11434R) react with?

Tested reactivity: Human, Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Rabbit.

How should bs-11434R be stored?

Shipped at 4℃. Store at -20°C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise MCT 8 Polyclonal Antibody (bs-11434R)?

KLH conjugated synthetic peptide derived from human MOT8/SLC16A2 Immunogen range: 101-200/539.

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