ABLIM3 Polyclonal Antibody
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-12442R-100uL |
| Product Name | ABLIM3 Polyclonal Antibody |
| Applications |
WB
ELISA
IHC-P
IHC-F
IF
ICC/IF
|
| Reactivity | Mouse |
| Predicted Reactivity | Human, Rat, Dog, Cow, Horse, Rabbit |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human ABLIM3 |
| Immunogen Range | 101-200/683 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. |
| Target | |
| Gene ID | 22885 |
| Subcellular location | Cytoplasm |
| Synonyms | abLIM-3; Ablim3; ABLM3_HUMAN; Actin binding LIM protein 3; Actin binding LIM protein family member 3; Actin-binding LIM protein 3; Actin-binding LIM protein family member 3; HMFN1661; KIAA0843. |
| Background | The ABLIM1 protein has an N-terminal domain that contains four double zinc finger motifs, which conform to the LIM motif consensus sequence. ABLIM1 binds to F-Actin through a dematin-like domain and is expressed in retina, brain and muscle tissue. There are four known isoforms of ABLIM1. The gene encoding ABLIM1 maps to a region of chromosome 10 associated with frequent loss of heterozygosity in human tumors, thus identifying ABLIM1 as a candidate tumor suppressor gene. ABLIM2 and ABLIM3 show highest expression in muscle and neuronal tissues, bind to F-Actin, and are localized on stress fibers. They also have been shown to enhance STARS (striated muscle activator of Rho signaling) dependent activation of serum-response factor (SRF), thereby modulating transcription. |
| Application Dilution | |
| WB | ELISA1:500-1000 |
| ELISA | IHC-P1:200-400 |
| IHC-P | IHC-F1:100-500 |
| IHC-F | IFIHC-P1:50-200 |
| IF | IHC-F1:50-200 |
| ICC/IF | IFICC1:50-200 |
Frequently asked questions
Which applications is ABLIM3 Polyclonal Antibody (bs-12442R) validated for, and at what dilution?
bs-12442R is validated for ELISA (1:500-1000); IHC-P (1:200-400); IHC-F (1:100-500); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200); ICC (1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does ABLIM3 Polyclonal Antibody (bs-12442R) react with?
Tested reactivity: Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Human, Rat, Dog, Cow, Horse, Rabbit.
How should bs-12442R be stored?
Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise ABLIM3 Polyclonal Antibody (bs-12442R)?
KLH conjugated synthetic peptide derived from human ABLIM3 Immunogen range: 101-200/683.