API5 Polyclonal Antibody

$Lane A: Mouse Heart lysate Lane B: Mouse Pancreas lysates probed with Rabbit Anti-API5 Polyclonal Antibody, Unconjugated (bs-1252R) at 1:300 overnight at 4˚C. Followed by HRP conjugated Goat-Anti-Rabbit IgG(bse-0295G-HRP) at 1:5000 for 90 min at 37˚C. \\n$

Lane A: Mouse Heart lysate Lane B: Mouse Pancreas lysates probed with Rabbit Anti-API5 Polyclonal Antibody, Unconjugated (bs-1252R) at 1:300 overnight at 4˚C. Followed by HRP conjugated Goat-Anti-Rabbit IgG(bse-0295G-HRP) at 1:5000 for 90 min at 37˚C. \\n

$Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with API5 Polyclonal Antibody, Unconjugated (bs-1252R) at 1:200 overnight at 4\u00b0C, DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with API5 Polyclonal Antibody, Unconjugated (bs-1252R) at 1:200 overnight at 4\u00b0C, DAB staining.

$Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with API5 Polyclonal Antibody, Unconjugated (bs-1252R) at 1:200 overnight at 4\u00b0C, DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with API5 Polyclonal Antibody, Unconjugated (bs-1252R) at 1:200 overnight at 4\u00b0C, DAB staining.

Applications

WB
IHC-P
IHC-F
IF

Reactivity

Mouse

Predicted Reactivity

Human
Rat
Dog
Cow
Pig
Horse
Guinea Pig

Overview
Catalog #	bs-1252R
Product Name	API5 Polyclonal Antibody
Applications	WB, IHC-P, IHC-F, IF
Reactivity	Mouse
Predicted Reactivity	Human, Rat, Dog, Cow, Pig, Horse, Guinea Pig
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human API5
Clonality	Polyclonal
Clone #	#REF!
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	8539
Swiss Prot	Q9BZZ5
Subcellular location	Cytoplasm, Nucleus
Synonyms	AAC11; AAC-11; Apoptosis inhibitor 5; API-5; Antiapoptosis clone 11 protein; Cell migration-inducing gene 8 protein; Fibroblast growth factor 2-interacting factor; FIF; Protein XAGL; API5; MIG8
Background	Antiapoptotic factor that may have a role in protein assembly. Negatively regulates ACIN1. By binding to ACIN1, it suppresses ACIN1 cleavage from CASP3 and ACIN1-mediated DNA fragmentation. Also known to efficiently suppress E2F1-induced apoptosis. Its depletion enhances the cytotoxic action of the chemotherapeutic drugs.
Application Dilution
WB	1:300-5000
IHC-P	ELISA1:500-1000
IHC-F	FCM1:20-100
IF