HIF 2 alpha Polyclonal Antibody, APC Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-1447R-APC-100uL |
| Product Name | HIF 2 alpha Polyclonal Antibody, APC Conjugated |
| Applications |
WB
FCM
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | APC |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from mouse HIF2 Alpha |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 13819 |
| Swiss Prot | P97481 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | HLF; HRF; MOP2; HIF2A; bHLHe73; HIF-2alpha; Endothelial PAS domain-containing protein 1; EPAS-1; HIF-1-alpha-like factor; mHLF; HIF-related factor; Hypoxia-inducible factor 2-alpha; HIF-2-alpha; HIF2-alpha; Epas1 |
| Background | Transcription factor involved in the induction of oxygen regulated genes. Binds to core DNA sequence 5'-[AG]CGTG-3' within the hypoxia response element (HRE) of target gene promoters. Regulates the vascular endothelial growth factor (VEGF) expression and seems to be implicated in the development of blood vessels and the tubular system of lung. May also play a role in the formation of the endothelium that gives rise to the blood brain barrier. Potent activator of the Tie-2 tyrosine kinase expression. Activation requires recruitment of transcriptional coactivators such as CREBPB and probably EP3. Interaction with redox regulatory protein APEX seems to activate CTAD (By similarity). |
| Application Dilution | |
| WB | 1:300-5000 |
| FCM | 1:20-100 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is HIF 2 alpha Polyclonal Antibody, APC Conjugated (bs-1447R-APC) validated for, and at what dilution?
bs-1447R-APC is validated for WB (1:300-5000); FCM (1:20-100); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does HIF 2 alpha Polyclonal Antibody, APC Conjugated (bs-1447R-APC) react with?
Tested reactivity: Human, Mouse, Rat.
How should bs-1447R-APC be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise HIF 2 alpha Polyclonal Antibody, APC Conjugated (bs-1447R-APC)?
KLH conjugated synthetic peptide derived from mouse HIF2 Alpha
What are the excitation and emission wavelengths of the APC conjugate (bs-1447R-APC)?
APC on bs-1447R-APC: excitation / emission 650nm/660nm.