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phospho-STAT1 (Tyr701) Polyclonal Antibody, AbBy Fluor-594 Conjugated

Applications

  • FCM

Reactivity

  • Human
  • Mouse

Predicted Reactivity

  • Rat
  • Dog
  • Cow
  • Sheep
  • Pig
  • Rabbit
Overview
Catalog # bs-1657R-BF594-100uL
Product Name phospho-STAT1 (Tyr701) Polyclonal Antibody, AbBy Fluor-594 Conjugated
Applications
FCM
Reactivity Human, Mouse
Predicted Reactivity Rat, Dog, Cow, Sheep, Pig, Rabbit
Specifications
Conjugation AbBy Fluor-594
Host Rabbit
Source KLH conjugated synthetic phosphopeptide derived from human STAT1 around the phosphorylation site of tyrosine 701
Modification Site Tyr701
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 6772
Subcellular location Cytoplasm, Nucleus
Synonyms STAT1Tyr701; signal transducers and activators of transcription 1; DKFZp686B04100; ISGF 3; Phosphorylated Stat1pTyr701; Signal transducer and activator of transcription 1 91kDa; Signal transducer and activator of transcription 1 alpha/beta; STAT 1; STAT 91; STAT91; Transcription factor ISGF 3 components p91 p84.
Background The protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein can be activated by various ligands including interferon-alpha, interferon-gamma, EGF, PDGF and IL6. This protein mediates the expression of a variety of genes, which is thought to be important for cell viability in response to different cell stimuli and pathogens. Two alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq].
Application Dilution
FCM 1:20-100

Frequently asked questions

Which applications is phospho-STAT1 (Tyr701) Polyclonal Antibody, AbBy Fluor-594 Conjugated (bs-1657R-BF594) validated for, and at what dilution?

bs-1657R-BF594 is validated for FCM (1:20-100). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does phospho-STAT1 (Tyr701) Polyclonal Antibody, AbBy Fluor-594 Conjugated (bs-1657R-BF594) react with?

Tested reactivity: Human, Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Rat, Dog, Cow, Sheep, Pig, Rabbit.

How should bs-1657R-BF594 be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise phospho-STAT1 (Tyr701) Polyclonal Antibody, AbBy Fluor-594 Conjugated (bs-1657R-BF594)?

KLH conjugated synthetic phosphopeptide derived from human STAT1 around the phosphorylation site of tyrosine 701

What are the excitation and emission wavelengths of the AbBy Fluor-594 conjugate (bs-1657R-BF594)?

AbBy Fluor-594 on bs-1657R-BF594: excitation / emission 590nm/617nm.

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US only. One sample per paid order, subject to review. Backorders may ship separately. Confirm Free at checkout. Check application and species suitability; same target does not guarantee equivalent performance. For research use only.

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