phospho-STAT3 (Tyr705) Polyclonal Antibody, Cy7 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-1658R-Cy7-100uL |
| Product Name | phospho-STAT3 (Tyr705) Polyclonal Antibody, Cy7 Conjugated |
| Applications |
WB
FCM
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human, Mouse, Rat, Chicken |
| Specifications | |
| Conjugation | Cy7 |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human STAT3 around the phosphorylation site of Tyr705 |
| Modification Site | Tyr705 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 6774 |
| Swiss Prot | P40763 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | APRF; HIES; ADMIO; Signal transducer and activator of transcription 3; Acute-phase response factor; STAT3 |
| Background | Signal transducer and transcription activator that mediates cellular responses to interleukins, KITLG/SCF and other growth factors. May mediate cellular responses to activated FGFR1, FGFR2, FGFR3 and FGFR4. Binds to the interleukin-6 (IL-6)-responsive elements identified in the promoters of various acute-phase protein genes. Activated by IL31 through IL31RA. Cytoplasmic STAT3 represses macroautophagy by inhibiting EIF2AK2/PKR activity. Plays an important role in host defense in methicillin-resistant S.aureus lung infection by regulating the expression of the antimicrobial lectin REG3G (By similarity). |
| Application Dilution | |
| WB | 1:300-5000 |
| FCM | 1:20-100 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is phospho-STAT3 (Tyr705) Polyclonal Antibody, Cy7 Conjugated (bs-1658R-Cy7) validated for, and at what dilution?
bs-1658R-Cy7 is validated for WB (1:300-5000); FCM (1:20-100); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does phospho-STAT3 (Tyr705) Polyclonal Antibody, Cy7 Conjugated (bs-1658R-Cy7) react with?
Tested reactivity: Human, Mouse, Rat, Chicken.
How should bs-1658R-Cy7 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise phospho-STAT3 (Tyr705) Polyclonal Antibody, Cy7 Conjugated (bs-1658R-Cy7)?
KLH conjugated synthetic phosphopeptide derived from human STAT3 around the phosphorylation site of Tyr705
What are the excitation and emission wavelengths of the Cy7 conjugate (bs-1658R-Cy7)?
Cy7 on bs-1658R-Cy7: excitation / emission 743nm/767nm.