alpha Actinin 4 Polyclonal Antibody

Lane 1: rat kidney lysates Lane 2: rat brain lysates probed with Anti alpha Actinin 4 Polyclonal Antibody, Unconjugated (bs-1741R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted band 105kD. Observed band size: 105kD.

$Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (alpha Actinin 4) polyclonal Antibody, Unconjugated (bs-1741R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.$

Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (alpha Actinin 4) polyclonal Antibody, Unconjugated (bs-1741R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

$Lane 1: Mouse Uterus tissue lysates; Lane 2: Rat Uterus tissue lysates probed with alpha Actinin 4 Polyclonal Antibody, Unconjugated (bs-1741R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.$

Lane 1: Mouse Uterus tissue lysates; Lane 2: Rat Uterus tissue lysates probed with alpha Actinin 4 Polyclonal Antibody, Unconjugated (bs-1741R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

WB
IHC-P
IHC-F
IF
Flow-Cyt

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bs-1741R
Product Name	alpha Actinin 4 Polyclonal Antibody
Applications	WB, IHC-P, IHC-F, IF, Flow-Cyt
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human Actinin alpha 4
Clonality	Polyclonal
Clone #	#REF!
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Store at -20C for 12 months.
Target
Gene ID	81
Synonyms	Actinin alpha 4; alpha-Actinin 4; alpha Actinin-4; actinin 4; actinin4; actinin-4; ACTN 4; ACTN4; DKFZp686K23158; F actin cross linking protein; FSGS 1; FSGS; FSGS1; Non muscle alpha actinin 4.
Background	Loading Control Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a nonmuscle, alpha actinin isoform which is concentrated in the cytoplasm, and thought to be involved in metastatic processes. Mutations in this gene have been associated with focal and segmental glomerulosclerosis. [provided by RefSeq, Jul 2008].
Application Dilution
WB	1:500-2000
IHC-P	FCM1:100
IHC-F	IHC-P1:100-500
IF	ICC1:100
Flow-Cyt