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RPS20 Polyclonal Antibody, Cy7 Conjugated

Applications

  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Rat
  • Yeast

Predicted Reactivity

  • Human
  • Mouse
  • Dog
  • Cow
  • Sheep
  • Pig
  • Horse
Overview
Catalog # bs-18322R-Cy7-100uL
Product Name RPS20 Polyclonal Antibody, Cy7 Conjugated
Applications
IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity Rat, Yeast
Predicted Reactivity Human, Mouse, Dog, Cow, Sheep, Pig, Horse
Specifications
Conjugation Cy7
Host Rabbit
Source KLH conjugated synthetic peptide derived from human RPS20
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 6224
Swiss Prot P60866
Subcellular location Cytoplasm
Synonyms S20; 40S ribosomal protein S20; RPS20
Background Mammalian ribosomal proteins are encoded by multigene families that consist of processed pseudogenes and one functional intron-containing gene within their coding regions. Ribosomal Protein S20, also known as RPS20, is a 119 amino acid cytoplasmic protein that is a component of the 40S ribosomal subunit. Co-transcribed with the small nucleolar RNA gene U54, Ribosomal Protein S20 is a primary binding protein (it binds independently to its target protein) that interacts with both the 5 and 3 minor domains of 16S ribosomal RNA (rRNA). Through its interactions with 16S rRNA, Ribosomal Protein S20 is thought to play a key role in nucleating the assembly of the 30S ribosomal subunit. Like most ribosomal protein-coding genes, the gene encoding Ribosomal Protein S20 is dispersed throughout the genome and exists as multiple processed pseudogenes.
Application Dilution
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is RPS20 Polyclonal Antibody, Cy7 Conjugated (bs-18322R-Cy7) validated for, and at what dilution?

bs-18322R-Cy7 is validated for IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does RPS20 Polyclonal Antibody, Cy7 Conjugated (bs-18322R-Cy7) react with?

Tested reactivity: Rat, Yeast. Predicted reactivity (expected from sequence homology but not yet tested): Human, Mouse, Dog, Cow, Sheep, Pig, Horse.

How should bs-18322R-Cy7 be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise RPS20 Polyclonal Antibody, Cy7 Conjugated (bs-18322R-Cy7)?

KLH conjugated synthetic peptide derived from human RPS20

What are the excitation and emission wavelengths of the Cy7 conjugate (bs-18322R-Cy7)?

Cy7 on bs-18322R-Cy7: excitation / emission 743nm/767nm.

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