5HT2A Receptor Polyclonal Antibody
Applications
Reactivity
| Overview | |
| Catalog # | bs-20743r-100uL |
| Product Name | 5HT2A Receptor Polyclonal Antibody |
| Applications |
ELISA
IHC-F
IF
IHC-P
|
| Reactivity | Human |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human 5HT2A Receptor |
| Immunogen Range | 21-120/471 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | Lot Dependent |
| Purification | purified by Protein A. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. |
| Target | |
| Gene ID | 3356 |
| Swiss Prot | P28223 |
| Subcellular location | Cell membrane |
| Synonyms | 5-hydroxytryptamine receptor 2A; 5-hydroxytryptamine receptor; Serotonin receptor 2A; 5-HT-2A; 5-HT-2; HTR2A; Htr2a; 5 HT 2; 5HTR2A; 5 HT 2A; 5 HT2 receptor; 5 HT2A; 5 hydroxytryptamine receptor 2A; 5-HT-2; 5-HT-2A; 5-hydroxytryptamine 2A receptor; 5-hydroxytryptamine receptor 2A; 5HT2A_HUMAN; HTR 2; HTR 2A; HTR2; HTR2A |
| Background | G-protein coupled receptor for 5-hydroxytryptamine (serotonin) (PubMed:1330647, PubMed:18703043, PubMed:19057895). Also functions as a receptor for various drugs and psychoactive substances, including mescaline, psilocybin, 1-(2,5-dimethoxy-4-iodophenyl)-2-aminopropane (DOI) and lysergic acid diethylamide (LSD) (PubMed:28129538). Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors (PubMed:28129538). Beta-arrestin family members inhibit signaling via G proteins and mediate activation of alternative signaling pathways (PubMed:28129538). Signaling activates phospholipase C and a phosphatidylinositol-calcium second messenger system that modulates the activity of phosphatidylinositol 3-kinase and promotes the release of Ca2+ ions from intracellular stores (PubMed:18703043, PubMed:28129538). Affects neural activity, perception, cognition and mood (PubMed:18297054). Plays a role in the regulation of behavior, including responses to anxiogenic situations and psychoactive substances. Plays a role in intestinal smooth muscle contraction, and may play a role in arterial vasoconstriction. |
| Application Dilution | |
| ELISA | =1:5000-10000 |
| IHC-F | IHC-F=1:100-500 |
| IF | IF=1:100-500 |
| IHC-P | IHC-P=1:100-500 |
Frequently asked questions
Which applications is 5HT2A Receptor Polyclonal Antibody (bs-20743R) validated for, and at what dilution?
bs-20743R is validated for ELISA (ELISA=1:5000-10000); IHC-F (IHC-F=1:100-500); IF (IF=1:100-500); IHC-P (IHC-P=1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does 5HT2A Receptor Polyclonal Antibody (bs-20743R) react with?
Tested reactivity: Human.
How should bs-20743R be stored?
Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise 5HT2A Receptor Polyclonal Antibody (bs-20743R)?
KLH conjugated synthetic peptide derived from human 5HT2A Receptor Immunogen range: 21-120/471.