ATOX1 Polyclonal Antibody

$Western blot analysis of extracts from normal (control) and ATOX1 knockout (KO) 293T cells, using ATOX1 antibody (bs-34235R) at 1:1000 dilution.\r\n Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.\r\n Lysates\/proteins: 25ug per lane.\r\n Blocking buffer: 3% nonfat dry milk in TBST.$

Western blot analysis of extracts from normal (control) and ATOX1 knockout (KO) 293T cells, using ATOX1 antibody (bs-34235R) at 1:1000 dilution.\r\n Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.\r\n Lysates\/proteins: 25ug per lane.\r\n Blocking buffer: 3% nonfat dry milk in TBST.

Applications

WB
ICC/IF

Reactivity

Human

Overview
Catalog #	bs-34235r
Product Name	ATOX1 Polyclonal Antibody
Applications	WB, ICC/IF
Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human ATOX1
Clonality	Polyclonal
Clone #	#REF!
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	475
Swiss Prot	O00244
Synonyms	Antioxidant 1 Copper Chaperone; HAH1; Copper Transport Protein ATOX1; Metal Transport Protein ATX1; ATX1; ATOX1_HUMAN.
Background	This gene encodes a copper chaperone that plays a role in copper homeostasis by binding and transporting cytosolic copper to ATPase proteins in the trans-Golgi network for later incorporation to the ceruloplasmin. This protein also functions as an antioxidant against superoxide and hydrogen peroxide, and therefore, may play a significant role in cancer carcinogenesis. Because of its cytogenetic location, this gene represents a candidate gene for 5q-syndrome. [provided by RefSeq, Jul 2008]
Application Dilution
WB	=1:500-2000
ICC/IF	ICC/IF=1:50-100