Phospho-Stat4 (Tyr693) Polyclonal Antibody, AbBy Fluor-555 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-3430R-BF555-100uL |
| Product Name | Phospho-Stat4 (Tyr693) Polyclonal Antibody, AbBy Fluor-555 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Mouse |
| Predicted Reactivity | Human, Rat, Dog, Cow, Pig, Horse, Chicken, Rabbit |
| Specifications | |
| Conjugation | AbBy Fluor-555 |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human Stat4 around the phosphorylation site of Tyr693 |
| Modification Site | Tyr693 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 6775 |
| Swiss Prot | Q14765 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | STAT4 Tyr693; STAT4 Y693; p-STAT4 Tyr693; signal transducers and activators of transduction 4; CIS4; HSPC060; SOCS4; SOCS6; SSI4; STAI4; STAT 4; STAT4; STATI4; Suppressor of cytokine signaling 6; STAT4_HUMAN. |
| Background | The protein encoded by this gene is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is essential for mediating responses to IL12 in lymphocytes, and regulating the differentiation of T helper cells. Mutations in this gene may be associated with systemic lupus erythematosus and rheumatoid arthritis. Alternate splicing results in multiple transcript variants that encode the same protein. [provided by RefSeq, Aug 2011]. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is Phospho-Stat4 (Tyr693) Polyclonal Antibody, AbBy Fluor-555 Conjugated (bs-3430R-BF555) validated for, and at what dilution?
bs-3430R-BF555 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Phospho-Stat4 (Tyr693) Polyclonal Antibody, AbBy Fluor-555 Conjugated (bs-3430R-BF555) react with?
Tested reactivity: Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Human, Rat, Dog, Cow, Pig, Horse, Chicken, Rabbit.
How should bs-3430R-BF555 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Phospho-Stat4 (Tyr693) Polyclonal Antibody, AbBy Fluor-555 Conjugated (bs-3430R-BF555)?
KLH conjugated synthetic phosphopeptide derived from human Stat4 around the phosphorylation site of Tyr693
What are the excitation and emission wavelengths of the AbBy Fluor-555 conjugate (bs-3430R-BF555)?
AbBy Fluor-555 on bs-3430R-BF555: excitation / emission 553nm/568nm.