Smad2/3 Polyclonal Antibody, Cy3 Conjugated

Applications

WB
FCM
IF(IHC-P)
IF(IHC-F)
IF(ICC)

Reactivity

Human
Mouse
Rat
Pig
Others

Predicted Reactivity

Cow
Chicken

Overview
Catalog #	bs-3484R-Cy3
Product Name	Smad2/3 Polyclonal Antibody, Cy3 Conjugated
Applications	WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)
Reactivity	Human, Mouse, Rat, Pig, Others
Predicted Reactivity	Cow, Chicken
Specifications
Conjugation	Cy3
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human Smad2
Immunogen Range	31-80/425
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage Condition	Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID	4087
Swiss Prot	Q15796
Subcellular location	Cytoplasm, Nucleus
Synonyms	Mothers against decapentaplegic homolog 2; MAD homolog 2; Mothers against DPP homolog 2; JV18-1; Mad-related protein 2; hMAD-2; SMAD family member 2; SMAD 2; Smad2; hSMAD2; SMAD2; MADH2; MADR2; SMAD2_HUMAN.
Background	Receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD2/SMAD4 complex, activates transcription. May act as a tumor suppressor in colorectal carcinoma. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator.
Application Dilution
WB	1:300-5000
FCM	1:20-100
IF(IHC-P)	1:50-200
IF(IHC-F)	1:50-200
IF(ICC)	1:50-200

Search our catalog of hundreds of thousands of products