Histone H3 (Acetyl K18) Polyclonal Antibody
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-3775R-100uL |
| Product Name | Histone H3 (Acetyl K18) Polyclonal Antibody |
| Applications |
WB
ELISA
FCM
|
| Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Cow, Pig, Rabbit, Fruit Fly |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | KLH conjugated synthetic acetylpeptide derived from human Histone H3 around acetylation site of Lys18 |
| Modification Site | K18 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| Target | |
| Gene ID | 3020 |
| Subcellular location | Nucleus |
| Synonyms | Acetyl-Histone H3Lys18; Histone H3Acetyl-Lys18; Histone H3Acetyl Lys18; Histone H3Acetyl-K18; H3 histone family member E pseudogene; H3F3; HIST3H3; Histone H3 3 pseudogene; H31_TETTH; Histone H3; H3S; Histone H3-I/H3-II; Major histone H3; H3F; Histone H3/a; Histone H3/b; Histone H3/c; Histone H3/d; Histone H3/f; Histone H3/h; Histone H3/i; Histone H3/j; Histone H3/k; Histone H3/l. |
| Background | Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6. [provided by RefSeq, Jul 2008]. |
| Application Dilution | |
| WB | 1:300-5000 |
| ELISA | 1:500-1000 |
| FCM | 1:20-100 |
Frequently asked questions
Which applications is Histone H3 (Acetyl K18) Polyclonal Antibody (bs-3775R) validated for, and at what dilution?
bs-3775R is validated for WB (1:300-5000); ELISA (1:500-1000); FCM (1:20-100). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Histone H3 (Acetyl K18) Polyclonal Antibody (bs-3775R) react with?
Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Rat, Cow, Pig, Rabbit, Fruit Fly.
How should bs-3775R be stored?
Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Histone H3 (Acetyl K18) Polyclonal Antibody (bs-3775R)?
KLH conjugated synthetic acetylpeptide derived from human Histone H3 around acetylation site of Lys18