RAE1 Polyclonal Antibody, HRP Conjugated
Applications
Predicted Reactivity
| Overview | |
| Catalog # | bs-3791R-HRP-100uL |
| Product Name | RAE1 Polyclonal Antibody, HRP Conjugated |
| Applications |
ELISA
IHC-P
IHC-F
|
| Predicted Reactivity | Human, Mouse, Rat, Dog, Cow, Pig, Horse, Chicken, Rabbit |
| Specifications | |
| Conjugation | HRP |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human RAE1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 8480 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | RAE-1; MIG 14; MIG14; Mnrp 41; Mnrp41; mRNA associated protein mrnp 41; mRNA binding protein 41 kD; mRNA export protein; MRNP 41; MRNP41; RAE 1; RAE1 RNA export 1 S.pombe homolog; RAE1 homolog; Rae1 protein homolog; RAE1 RNA export 1 homolog; RNA export 1; RNA export 1 homolog; Homolog of yeast Rae1 mRNA associated protein of 41 kDa. RAE1L_HUMAN |
| Background | Mutations in Rae1 and Gle2(Saccharomyces cerevisae homolog) genes have been shown to result in accumulation of poly(A)-containing mRNA in the nucleus, suggesting that the encoded proteins are involved in RNA export. It contains four WD40 motifs, and has been shown to localize to distinct foci in the nucleoplasm, to the nuclear rim, and to meshwork-like structures throughout the cytoplasm. This gene is thought to be involved in nucleocytoplasmic transport, and in directly or indirectly attaching cytoplasmic mRNPs to the cytoskeleton. Depletion of Rae1 from extracts or cells severely inhibits mitotic spindle assembly. Alternatively spliced transcript variants encoding the same protein have been found for this gene. |
| Application Dilution | |
| ELISA | 1:500-1000 |
| IHC-P | 1:200-400 |
| IHC-F | 1:100-500 |
Frequently asked questions
Which applications is RAE1 Polyclonal Antibody, HRP Conjugated (bs-3791R-HRP) validated for, and at what dilution?
bs-3791R-HRP is validated for ELISA (1:500-1000); IHC-P (1:200-400); IHC-F (1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
How should bs-3791R-HRP be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise RAE1 Polyclonal Antibody, HRP Conjugated (bs-3791R-HRP)?
KLH conjugated synthetic peptide derived from human RAE1