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AK2 Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded Human gastric carcinoma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with AK2 Polyclonal Antibody, Unconjugated (bs-55004R) at 1:100 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human gastric carcinoma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with AK2 Polyclonal Antibody, Unconjugated (bs-55004R) at 1:100 overnight at 4\u00b0C, DAB staining.
Lane 1: 293T cell lysates; Lane 2: AK2 knockout (KO) 293T cell lysates probed with AK2 Polyclonal Antibody, Unconjugated (bs-55004R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: 293T cell lysates; Lane 2: AK2 knockout (KO) 293T cell lysates probed with AK2 Polyclonal Antibody, Unconjugated (bs-55004R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: HL-60 cell lysates; Lane 2: Mouse Liver lysates; Lane 3: Mouse Kidney lysates; Lane 4: Rat Liver lysates probed with AK2 Polyclonal Antibody, Unconjugated (bs-55004R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: HL-60 cell lysates; Lane 2: Mouse Liver lysates; Lane 3: Mouse Kidney lysates; Lane 4: Rat Liver lysates probed with AK2 Polyclonal Antibody, Unconjugated (bs-55004R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

  • WB
  • IHC-P
  • IHC

Reactivity

  • Human
  • Mouse
  • Rat
Overview
Catalog # bs-55004R
Product Name AK2 Polyclonal Antibody
Applications WB, IHC-P, IHC
Reactivity Human, Mouse, Rat
Specifications
Conjugation Unconjugated
Host Rabbit
Source Recombinant fusion protein containing a sequence corresponding to amino acids 1-232 of human AK2 (NP_001616.1).
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20°C for 12 months.
Target
Gene ID 204
Swiss Prot P54819
Subcellular location Cytoplasm, Cell membrane
Synonyms AK2;ADK2
Background Adenylate kinases are involved in regulating the adenine nucleotide composition within a cell by catalyzing the reversible transfer of phosphate groups among adenine nucleotides. Three isozymes of adenylate kinase, namely 1, 2, and 3, have been identified in vertebrates; this gene encodes isozyme 2. Expression of these isozymes is tissue-specific and developmentally regulated. Isozyme 2 is localized in the mitochondrial intermembrane space and may play a role in apoptosis. Mutations in this gene are the cause of reticular dysgenesis. Alternate splicing results in multiple transcript variants. Pseudogenes of this gene are found on chromosomes 1 and 2.
Application Dilution
WB 1:300-5000
IHC-P 1:200-400
IHC

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