ARHGDIA Polyclonal Antibody

$Lane 1: Hela cell lysates; Lane 2: COS-7 cell lysates; Lane 3: PC-12 cell lysates; Lane 4: Mouse lung lysates; Lane 5: Mouse spleen lysates; Lane 6: Mouse kidney lysates probed with ARHGDIA Polyclonal Antibody, Unconjugated (bs-55013R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.\r\n$

Lane 1: Hela cell lysates; Lane 2: COS-7 cell lysates; Lane 3: PC-12 cell lysates; Lane 4: Mouse lung lysates; Lane 5: Mouse spleen lysates; Lane 6: Mouse kidney lysates probed with ARHGDIA Polyclonal Antibody, Unconjugated (bs-55013R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.\r\n

Lane 1: 293T cell lysates; Lane 2: ARHGDIA knockout (KO) 293T cell lysates probed with ARHGDIA Polyclonal Antibody, Unconjugated (bs-55013R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

$Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (ARHGDIA) polyclonal Antibody, Unconjugated (bs-55013R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.$

Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (ARHGDIA) polyclonal Antibody, Unconjugated (bs-55013R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

Applications

WB
IF(ICC)
IF

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bs-55013R
Product Name	ARHGDIA Polyclonal Antibody
Applications	WB, IF(ICC), IF
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Recombinant fusion protein containing a sequence corresponding to amino acids 1-204 of human ARHGDIA (NP_001172006.1).
Clonality	Polyclonal
Clone #	#REF!
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Store at -20C for 12 months.
Target
Gene ID	396
Swiss Prot	P52565
Subcellular location	Cytoplasm
Synonyms	ARHGDIA;GDIA1;HEL-S-47e;NPHS8;RHOGDI;RHOGDI-1
Background	This gene encodes a protein that plays a key role in the regulation of signaling through Rho GTPases. The encoded protein inhibits the disassociation of Rho family members from GDP (guanine diphosphate), thereby maintaining these factors in an inactive state. Activity of this protein is important in a variety of cellular processes, and expression of this gene may be altered in tumors. Mutations in this gene have been found in individuals with nephrotic syndrome, type 8. Alternate splicing results in multiple transcript variants.
Application Dilution
WB	1:300-5000
IF(ICC)	1:50-200
IF