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Fast Myosin Skeletal Heavy chain Polyclonal Antibody

Applications

  • ELISA
  • IHC-P
  • IF(ICC)

Predicted Reactivity

  • Human
  • Mouse
  • Rat
  • Dog
  • Pig
  • Chicken
Overview
Catalog # bs-5885R-100uL
Product Name Fast Myosin Skeletal Heavy chain Polyclonal Antibody
Applications
ELISA IHC-P IF(ICC)
Predicted Reactivity Human, Mouse, Rat, Dog, Pig, Chicken
Specifications
Conjugation Unconjugated
Host Rabbit
Source KLH conjugated synthetic peptide derived from human Myosin heavy chain 1
Immunogen Range 901-1000/1939
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Target
Subcellular location Cytoplasm
Synonyms adult 1; MGC133384; Myh 1; Myh1; MYH1_HUMAN; MyHC-IIx/d; MYHSA1; MYL1; Myosin 1; Myosin heavy chain 1; Myosin heavy chain 1 skeletal muscle adult; Myosin heavy chain; Myosin heavy chain IIx/d; Myosin heavy chain skeletal muscle adult 1; Myosin light chain 1 alkali skeletal fast; Myosin-1; Myosin1; skeletal muscle.
Background Myosin is a protein which is known to interact with actin in muscle and non-muscle cells. It contains two identical heavy chains and four light chains. Myosin molecules consist of two major regions: tails (rods) and heads. They aggregate into filaments through the tail region and interact with actin and with ATP through the head region. Multiple forms of myosin heavy chains exist for each muscle type-skeletal, cardiac, smooth and non-muscle isomyosin forms exist in different types of skeletal muscle, depending on the physiological function of the muscle. They are designated at type I (slow twitch) and type II (fast twitch).
Application Dilution
ELISA 1:500-1000
IHC-P 1:200-400
IF(ICC) 1:50-200

Frequently asked questions

Which applications is Fast Myosin Skeletal Heavy chain Polyclonal Antibody (bs-5885R) validated for, and at what dilution?

bs-5885R is validated for ELISA (1:500-1000); IHC-P (1:200-400); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

How should bs-5885R be stored?

Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise Fast Myosin Skeletal Heavy chain Polyclonal Antibody (bs-5885R)?

KLH conjugated synthetic peptide derived from human Myosin heavy chain 1 Immunogen range: 901-1000/1939.

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