Tri-Methyl-Histone H3 (Lys4) Polyclonal Antibody

$Blocking buffer: 5% NFDM\/TBST\r\nPrimary ab dilution: 1:2000\r\nPrimary ab incubation condition: 2 hours at room temperature\r\nSecondary ab: Goat Anti-Rabbit IgG H&L (HRP)\r\nLysate: HeLa, C2C12, COS-7\r\nProtein loading quantity: 20 \u03bcg\r\nExposure time: 30 s\r\nPredicted MW: 17 kDa\r\nObserved MW: 17 kDa$

Blocking buffer: 5% NFDM\/TBST\r\nPrimary ab dilution: 1:2000\r\nPrimary ab incubation condition: 2 hours at room temperature\r\nSecondary ab: Goat Anti-Rabbit IgG H&L (HRP)\r\nLysate: HeLa, C2C12, COS-7\r\nProtein loading quantity: 20 \u03bcg\r\nExposure time: 30 s\r\nPredicted MW: 17 kDa\r\nObserved MW: 17 kDa

$Tissue: Mouse colon\r\nSection type: Formalin fixed & Paraffin -embedded section\r\nRetrieval method: High temperature and high pressure\r\nRetrieval buffer: Tris\/EDTA buffer, pH 9.0 Primary ab dilution: 1:500\r\nPrimary ab incubation condition: 1 hour at room temperature\r\nSecondary ab: SP Kit(Rabbit) (sp-0023)\r\nCounter stain: Hematoxylin (Blue)\r\nComment: Color brown is the positive signal for bs-60099R\r\n$

Tissue: Mouse colon\r\nSection type: Formalin fixed & Paraffin -embedded section\r\nRetrieval method: High temperature and high pressure\r\nRetrieval buffer: Tris\/EDTA buffer, pH 9.0 Primary ab dilution: 1:500\r\nPrimary ab incubation condition: 1 hour at room temperature\r\nSecondary ab: SP Kit(Rabbit) (sp-0023)\r\nCounter stain: Hematoxylin (Blue)\r\nComment: Color brown is the positive signal for bs-60099R\r\n

$Chromatin immunoprecipitation was performed using cross-linked chromatin from 5\u00d710\u2076 HeLa cells per IP, with 2 \u03bcg of bs-60099R antibody or 2 \u03bcg of normal rabbit IgG(negative control bs-0295P) , and 20 \u03bcL of Protein A\/G MagBeads per IP. The immunoprecipitated DNA was quantified by real-time PCR using primers specific for the human GAPDH-CDS and RPL30 exon 4 regions. The data are presented as enrichment of each sample relative to the total amount of input at each amplicon.$

Chromatin immunoprecipitation was performed using cross-linked chromatin from 5\u00d710\u2076 HeLa cells per IP, with 2 \u03bcg of bs-60099R antibody or 2 \u03bcg of normal rabbit IgG(negative control bs-0295P) , and 20 \u03bcL of Protein A\/G MagBeads per IP. The immunoprecipitated DNA was quantified by real-time PCR using primers specific for the human GAPDH-CDS and RPL30 exon 4 regions. The data are presented as enrichment of each sample relative to the total amount of input at each amplicon.

Applications

WB
IHC-F
IF
ChIP
IHC-P

Reactivity

Human
Mouse
Rat
Monkey

Overview
Catalog #	bs-60099r
Product Name	Tri-Methyl-Histone H3 (Lys4) Polyclonal Antibody
Applications	WB, IHC-F, IF, ChIP, IHC-P
Reactivity	Human, Mouse, Rat, Monkey
Specifications
Conjugation	Unconjugated
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Concentration	Lot Dependent
Purification	Protein A coupled with the antigen peptide containing tri-methylated H3 (Lys4).
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Swiss Prot	P68431
Application Dilution
WB	1:500-2000
IHC-F	1:50-200
IF	1:50-200
ChIP	2μg/IP
IHC-P	1:50-200