F(ab')₂ Fragment Goat Anti-Rabbit IgG (H+L)
Applications
Reactivity
| Overview | |
| Catalog # | bs-60295g |
| Product Name | F(ab')₂ Fragment Goat Anti-Rabbit IgG (H+L) |
| Applications |
WB
IHC
IF
|
| Reactivity | Rabbit |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | Lot Dependent |
| Purification | Purified by Protein G. |
| Storage Buffer | 0.01M PBS(pH7.4) |
| Storage Condition | Shipped at 4°. Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Target | |
| Synonyms | F(ab)2 Fragment Goat Anti-Rabbit IgG(H+L); F(ab')₂ Fragment Goat Anti-Rabbit IgG (H+L); F(ab')₂ Fragment Goat Anti-Rabbit IgG H&L; |
| Background | Immunoglobulin G (IgG), is one of the most abundant proteins in serum with normal levels between 8-17 mg/mL in adult blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants. |
| Application Dilution | |
| WB | 1:300-5000 |
| IHC | 1:50-200 |
| IF | 1:50-200 |
Frequently asked questions
Which applications is F(ab')₂ Fragment Goat Anti-Rabbit IgG (H+L) (bs-60295G) validated for, and at what dilution?
bs-60295G is validated for WB (1:300-5000); IHC (1:50-200); IF (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does F(ab')₂ Fragment Goat Anti-Rabbit IgG (H+L) (bs-60295G) react with?
Tested reactivity: Rabbit.
How should bs-60295G be stored?
Shipped at 4°. Store at -20°C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M PBS(pH7.4)