AKT (Thr342) Antibody

Applications

Reactivity

Drosophila

Overview
Catalog #	bs-70012r
Product Name	AKT (Thr342) Antibody
Applications	WB
Specificity	Specific for endogenous levels of the ~68 kDa AKT protein phosphorylated at Thr342. Immunolabeling is completely eliminated with _-phosphatase treatment. It has been reported that this antibody may also recognize some level of phosphorylated S6K as there is 67% homology with the sequence used as antigen.
Reactivity	Drosophila
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr342 of Drosophila AKT, conjugated to keyhole limpet hemocyanin (KLH)
Modification Site	Thr342
Clonality	Polyclonal
Clone #	#REF!
Isotype	IgG
Concentration	Lot Dependent
Purification	Antigen Affinity purification from Pooled whole antiserum
Storage Buffer	10 mM HEPES (pH 7.5), 150 mM NaCl, 100 g per ml BSA and 50% glycerol.
Storage Condition	Storage at -20C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20C.
Target
Gene ID	41957
Swiss Prot	Q8INB9
Synonyms	AKT1 antibody, AKT1_HUMAN antibody, AKT2 antibody, AKT3 antibody, PKB alpha antibody, PKB antibody, PKB beta antibody, PKBalpha antibody, PRKBA antibody, PRKBB antibody, PRKBG antibody, Protein kinase B antibody, Protein kinase B beta antibody, Protein kinase B gamma antibody, Proto-oncogene c-Akt antibody, RAC alpha antibody, RAC antibody, RAC gamma antibody, RAC PK alpha antibody, RAC PK beta antibody, RAC PK gamma antibody, RAC-alpha serine/threonine-protein kinase antibody, RAC-PK-alpha antibody, STK2 antibody
Background	The serine/threonine kinase Akt also known as protein kinase B (PKB) or Rac, plays a crucial role in controlling many diverse and important cellular functions such as cell survival and glycogen metabolism (Hajduch et al., 2001 and Nicholson & Anderson, 2002). Three isoforms (_, _, and _) have been identified that can be activated rapidly in response to insulin and growth factors in a phosphoinositide 3-kinase (PI3K)-dependent fashion (Hajduch et al., 2003). Phosphorylation of Akt_ occurs at two specific regulatory sites in Drosophila, one localized in the kinase domain, Thr342, and the other in the C-terminal regulatory domain, Ser505: these two activation sites are homologous to mammalian Ser473 and Thr308 respectively (Powell et al., 2004).
Application Dilution
WB	1:300-5000