Arginase I (ARG1) Antibody
Applications
Reactivity
| Overview | |
| Catalog # | bs-70021ch-100uL |
| Product Name | Arginase I (ARG1) Antibody |
| Applications |
WB
IHC
|
| Specificity | Specific for endogenous levels of the ~35-38 kDa arginase I protein. |
| Reactivity | Human, Mouse, Rat, Guinea Pig |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Chicken |
| Source | Full-length recombinant rat liver arginase I expressed in and purified from E. Coli. |
| Clonality | Polyclonal |
| Isotype | IgY |
| Concentration | Lot Dependent |
| Purification | Total IgY fraction |
| Storage Buffer | 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol. |
| Storage Condition | Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. |
| Target | |
| Gene ID | 29221 |
| Swiss Prot | P07824 |
| Synonyms | A I antibody, Al antibody, ARG 1 antibody, arg1 antibody, ARGI1_HUMAN antibody, Arginase 1 antibody, Arginase liver antibody, Arginase type I antibody, Arginase liver antibody, Arginase-1 antibody, Arginase1 antibody, Liver type arginase antibody, Liver-type arginase antibody, Type I arginase antibody |
| Background | Arginase I, a cytosolic enzyme, is highly expressed in the liver and is a component of the urea cycle (Jenkinson et al, 1996). There are two isoforms of arginase, both catalyze the conversion of arginine to ornithine and urea, but differ based on subcellular localization, tissue distribution, and enzymatic properties (Jenkinson et al, 1996). Arginase I has been identified in macrophages in response to various pathogen exposures causing an upregulation of inducible nitric oxide synthase, iNOS, yielding increased levels of nitric oxide (Morris et al, 1998) and inflammation (Poljakovic et al, 2007). |
| Application Dilution | |
| WB | 1:300-5000 |
| IHC | |
Frequently asked questions
Which applications is Arginase I (ARG1) Antibody (bs-70021CH) validated for, and at what dilution?
bs-70021CH is validated for WB (1:300-5000); IHC. These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Arginase I (ARG1) Antibody (bs-70021CH) react with?
Tested reactivity: Human, Mouse, Rat, Guinea Pig.
How should bs-70021CH be stored?
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. Storage buffer: 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.
What immunogen was used to raise Arginase I (ARG1) Antibody (bs-70021CH)?
Full-length recombinant rat liver arginase I expressed in and purified from E. Coli.