DOPA Decarboxylase Antibody
Applications
Reactivity
| Overview | |
| Catalog # | bs-70066r-100uL |
| Product Name | DOPA Decarboxylase Antibody |
| Applications |
WB
|
| Specificity | Specific for endogenous levels of the ~55 kDa DDC protein. |
| Reactivity | Human, Rat, Bovine, Dog, Sheep, Rabbit, Guinea Pig |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | Synthetic peptide from N-terminus region of human DOPA decarboxylase (DDC), conjugated to keyhole limpet hemocyanin (KLH). |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | Lot Dependent |
| Purification | Antigen Affinity purification from Pooled whole antiserum |
| Storage Buffer | 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol. |
| Storage Condition | Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. |
| Target | |
| Gene ID | 1644 |
| Swiss Prot | P20711 |
| Synonyms | AADC antibody, Aromatic L Amino Acid Decarboxylase antibody, Aromatic-L-amino-acid decarboxylase antibody, DDC antibody, DDC_HUMAN antibody, DOPA decarboxylase (aromatic L-amino acid decarboxylase) antibody, DOPA decarboxylase antibody |
| Background | DOPA decarboxylase (aromatic L-amino acid decarboxylase, AADC; DDC) catalyzes the second reaction in the biosynthesis of catecholamines and serotonin (Waymire and Haycock, 2002; Berry et al., 1996; Haycock et al., 2003). It is also involved in the biosynthesis of trace amines. DDC antibodies can therefore be used as markers for dopaminergic, noradrenergic and serotonergic neurons in a variety of applications including depression, schizophrenia, Parkinson’s disease and drug abuse (Kish et al., 2001; Zhu et al., 2000; Zhu et al., 1999). |
| Application Dilution | |
| WB | 1:300-5000 |
Frequently asked questions
Which applications is DOPA Decarboxylase Antibody (bs-70066R) validated for, and at what dilution?
bs-70066R is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does DOPA Decarboxylase Antibody (bs-70066R) react with?
Tested reactivity: Human, Rat, Bovine, Dog, Sheep, Rabbit, Guinea Pig.
How should bs-70066R be stored?
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. Storage buffer: 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.
What immunogen was used to raise DOPA Decarboxylase Antibody (bs-70066R)?
Synthetic peptide from N-terminus region of human DOPA decarboxylase (DDC), conjugated to keyhole limpet hemocyanin (KLH).