Microtubule Associated Protein 2 (MAP2) Antibody
Applications
Reactivity
| Overview | |
| Catalog # | bs-70141g-100uL |
| Product Name | Microtubule Associated Protein 2 (MAP2) Antibody |
| Applications |
WB
IHC
|
| Specificity | Specific for endogenous levels of the ~280 kDa MAP2 protein. |
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Goat |
| Source | Recombinant human MAP2 protein expressed in and purified from E. Coli. |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | Lot Dependent |
| Purification | Purified by Protein G. |
| Storage Buffer | PBS + 50% glycerol and 5 mM NaN3 |
| Storage Condition | Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. |
| Target | |
| Gene ID | 4133 |
| Swiss Prot | P11137 |
| Synonyms | DKFZp686I2148 antibody, MAP 2 antibody, MAP dendrite specific antibody, MAP-2 antibody, MAP2 antibody, MAP2A antibody, MAP2B antibody, MAP2C antibody, Microtubule associated protein 2 antibody, Microtubule-associated protein 2 antibody, MTAP2_HUMAN antibody |
| Background | Microtubules are 25nm diameter protein rods found in most kinds of eukaryotic cells. They are polymerized from a dimeric subunit made of one a subunit and one b tubulin subunit. Microtubules are associated with a family of proteins called microtubule associated proteins (MAPs), which includes the protein t (tau) and a group of proteins referred to as MAP1, MAP2, MAP3, MAP4 and MAP5 (Kindler & Gardner 1994). MAP2 is made up of two ~280 kDa apparent molecular weight bands referred to as MAP2a and MAP2b. A third lower molecular weight form, usually called MAP2c, corresponds to a pair of protein bands running at ~70 kDa on SDS-PAGE gels. All these MAP2 forms are derived from a single gene by alternate transcription, and all share a C-terminal sequence which includes either three or four microtubule binding peptide sequences, which are very similar to those found in the related microtubule binding protein t (tau). MAP2 isoforms are expressed only in neuronal cells and specifically in the perikarya and dendrites of these cells. MAP2 has been recently shown to be the specific receptor for the neurosteroid pregnenolone (Fontaine-Lenore V. et al., 2006). |
| Application Dilution | |
| WB | 1:300-5000 |
| IHC | |
Frequently asked questions
Which applications is Microtubule Associated Protein 2 (MAP2) Antibody (bs-70141G) validated for, and at what dilution?
bs-70141G is validated for WB (1:300-5000); IHC. These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Microtubule Associated Protein 2 (MAP2) Antibody (bs-70141G) react with?
Tested reactivity: Human, Mouse, Rat.
How should bs-70141G be stored?
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. Storage buffer: PBS + 50% glycerol and 5 mM NaN3
What immunogen was used to raise Microtubule Associated Protein 2 (MAP2) Antibody (bs-70141G)?
Recombinant human MAP2 protein expressed in and purified from E. Coli.